A Mixture of D-Amino Acids Enhances the Biocidal Efficacy of CMIT/MIT Against Corrosive Vibrio harveyi Biofilm
Biocides are widely used for the mitigation of microbial contamination, especially in the field of the aviation fuel industry. However, the long-term use of biocide has raised the concerns regarding the environmental contamination and microbial drug resistance. In this study, the effect of a mixture...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.557435/full |
| _version_ | 1811226072345214976 |
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| author | Xiaomeng Liu Xiaomeng Liu Xiaomeng Liu Zhong Li Zhong Li Yongqiang Fan Yongqiang Fan Yassir Lekbach Yassir Lekbach Yongbo Song Dake Xu Dake Xu Zhichao Zhang Lei Ding Fuhui Wang Fuhui Wang |
| author_facet | Xiaomeng Liu Xiaomeng Liu Xiaomeng Liu Zhong Li Zhong Li Yongqiang Fan Yongqiang Fan Yassir Lekbach Yassir Lekbach Yongbo Song Dake Xu Dake Xu Zhichao Zhang Lei Ding Fuhui Wang Fuhui Wang |
| author_sort | Xiaomeng Liu |
| collection | DOAJ |
| description | Biocides are widely used for the mitigation of microbial contamination, especially in the field of the aviation fuel industry. However, the long-term use of biocide has raised the concerns regarding the environmental contamination and microbial drug resistance. In this study, the effect of a mixture of D-amino acids (D-tyrosine and D-methionine) on the enhancement of the bactericidal effect of 5-Chloro-2-Methyl-4-isothiazolin-3-one/2-Methyl-2H-isothiazole-3-one (CMIT/MIT) against corrosive Vibrio harveyi biofilm was evaluated. The results revealed that D-Tyr and D-Met alone can enhance the biocidal efficacy of CMIT/MIT, while the treatment of 5 ppm CMIT/MIT, 1 ppm D-Tyr and 100 ppm D-Met showed the best efficacy comparable to that of 25 ppm CMIT/MIT alone. The triple combination treatment successfully prevented the establishment of the corrosive V. harveyi biofilm and effectively removed the mature V. harveyi biofilm. These conclusions were confirmed by the results of sessile cell counts, images obtained by scanning electron microscope and confocal laser scanning microscope, and the ATP test kit. |
| first_indexed | 2024-04-12T09:18:13Z |
| format | Article |
| id | doaj.art-656440195a6345d28293ebda93bdec0c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T09:18:13Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-656440195a6345d28293ebda93bdec0c2022-12-22T03:38:46ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-09-011110.3389/fmicb.2020.557435557435A Mixture of D-Amino Acids Enhances the Biocidal Efficacy of CMIT/MIT Against Corrosive Vibrio harveyi BiofilmXiaomeng Liu0Xiaomeng Liu1Xiaomeng Liu2Zhong Li3Zhong Li4Yongqiang Fan5Yongqiang Fan6Yassir Lekbach7Yassir Lekbach8Yongbo Song9Dake Xu10Dake Xu11Zhichao Zhang12Lei Ding13Fuhui Wang14Fuhui Wang15School of Life Sciences and Biopharmaceuticals, Shenyang Pharmaceutical University, Shenyang, ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, ChinaShenyang National Laboratory for Materials Science, Northeastern University, Shenyang, ChinaShenyang National Laboratory for Materials Science, Northeastern University, Shenyang, ChinaKey Laboratory for Anisotropy and Texture of Materials (Ministry of Education), School of Materials Science and Engineering, Northeastern University, Shenyang, ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, ChinaShenyang National Laboratory for Materials Science, Northeastern University, Shenyang, ChinaShenyang National Laboratory for Materials Science, Northeastern University, Shenyang, ChinaKey Laboratory for Anisotropy and Texture of Materials (Ministry of Education), School of Materials Science and Engineering, Northeastern University, Shenyang, ChinaSchool of Life Sciences and Biopharmaceuticals, Shenyang Pharmaceutical University, Shenyang, ChinaShenyang National Laboratory for Materials Science, Northeastern University, Shenyang, ChinaKey Laboratory for Anisotropy and Texture of Materials (Ministry of Education), School of Materials Science and Engineering, Northeastern University, Shenyang, ChinaShenyang Aircraft Design and Research Institute, Shenyang, ChinaShenyang Aircraft Design and Research Institute, Shenyang, ChinaShenyang National Laboratory for Materials Science, Northeastern University, Shenyang, ChinaKey Laboratory for Anisotropy and Texture of Materials (Ministry of Education), School of Materials Science and Engineering, Northeastern University, Shenyang, ChinaBiocides are widely used for the mitigation of microbial contamination, especially in the field of the aviation fuel industry. However, the long-term use of biocide has raised the concerns regarding the environmental contamination and microbial drug resistance. In this study, the effect of a mixture of D-amino acids (D-tyrosine and D-methionine) on the enhancement of the bactericidal effect of 5-Chloro-2-Methyl-4-isothiazolin-3-one/2-Methyl-2H-isothiazole-3-one (CMIT/MIT) against corrosive Vibrio harveyi biofilm was evaluated. The results revealed that D-Tyr and D-Met alone can enhance the biocidal efficacy of CMIT/MIT, while the treatment of 5 ppm CMIT/MIT, 1 ppm D-Tyr and 100 ppm D-Met showed the best efficacy comparable to that of 25 ppm CMIT/MIT alone. The triple combination treatment successfully prevented the establishment of the corrosive V. harveyi biofilm and effectively removed the mature V. harveyi biofilm. These conclusions were confirmed by the results of sessile cell counts, images obtained by scanning electron microscope and confocal laser scanning microscope, and the ATP test kit.https://www.frontiersin.org/article/10.3389/fmicb.2020.557435/fullD-amino acidD-tyrosineD-methionineVibrio harveyibiocide |
| spellingShingle | Xiaomeng Liu Xiaomeng Liu Xiaomeng Liu Zhong Li Zhong Li Yongqiang Fan Yongqiang Fan Yassir Lekbach Yassir Lekbach Yongbo Song Dake Xu Dake Xu Zhichao Zhang Lei Ding Fuhui Wang Fuhui Wang A Mixture of D-Amino Acids Enhances the Biocidal Efficacy of CMIT/MIT Against Corrosive Vibrio harveyi Biofilm Frontiers in Microbiology D-amino acid D-tyrosine D-methionine Vibrio harveyi biocide |
| title | A Mixture of D-Amino Acids Enhances the Biocidal Efficacy of CMIT/MIT Against Corrosive Vibrio harveyi Biofilm |
| title_full | A Mixture of D-Amino Acids Enhances the Biocidal Efficacy of CMIT/MIT Against Corrosive Vibrio harveyi Biofilm |
| title_fullStr | A Mixture of D-Amino Acids Enhances the Biocidal Efficacy of CMIT/MIT Against Corrosive Vibrio harveyi Biofilm |
| title_full_unstemmed | A Mixture of D-Amino Acids Enhances the Biocidal Efficacy of CMIT/MIT Against Corrosive Vibrio harveyi Biofilm |
| title_short | A Mixture of D-Amino Acids Enhances the Biocidal Efficacy of CMIT/MIT Against Corrosive Vibrio harveyi Biofilm |
| title_sort | mixture of d amino acids enhances the biocidal efficacy of cmit mit against corrosive vibrio harveyi biofilm |
| topic | D-amino acid D-tyrosine D-methionine Vibrio harveyi biocide |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.557435/full |
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