Comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix-alginate scaffold for better follicle development in vitro
Abstract Background Nowadays, the number of cancer survivors is significantly increasing as a result of efficient chemo/radio therapeutic treatments. Female cancer survivors may suffer from decreased fertility. In this regard, different fertility preservation techniques were developed. Artificial ov...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-01-01
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| Series: | BMC Biotechnology |
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| Online Access: | https://doi.org/10.1186/s12896-020-00658-3 |
| _version_ | 1818665011379175424 |
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| author | Hossein Nikniaz Zahra Zandieh Mohammad Nouri Neda Daei-farshbaf Reza Aflatoonian Mazaher Gholipourmalekabadi Seyed Behnamedin Jameie |
| author_facet | Hossein Nikniaz Zahra Zandieh Mohammad Nouri Neda Daei-farshbaf Reza Aflatoonian Mazaher Gholipourmalekabadi Seyed Behnamedin Jameie |
| author_sort | Hossein Nikniaz |
| collection | DOAJ |
| description | Abstract Background Nowadays, the number of cancer survivors is significantly increasing as a result of efficient chemo/radio therapeutic treatments. Female cancer survivors may suffer from decreased fertility. In this regard, different fertility preservation techniques were developed. Artificial ovary is one of these methods suggested by several scientific groups. Decellularized ovarian cortex has been introduced as a scaffold in the field of human fertility preservation. This study was carried out to compare decellularization of the ovarian scaffold by various protocols and evaluate the follicle survival in extracellular matrix (ECM)-alginate scaffold. Results The micrographs of H&E and DAPI staining confirmed successful decellularization of the ovarian cortex in all experimental groups, but residual DNA content in SDS-Triton group was significantly higher than other groups (P < 0.05). SEM images demonstrated that complex fiber network and porosity structure were maintained in all groups. Furthermore, elastin and collagen fibers were observed in all groups after decellularization process. MTT test revealed higher cytobiocompatibility of the SDS-Triton-Ammonium and SDS-Triton decellularized scaffolds compared with SDS groups. Compared to the transferred follicles into the sodium alginate (81%), 85.9% of the transferred follicles into the decellularized scaffold were viable after 7 days of cultivation (P = 0.04). Conclusion Although all the decellularization procedures was effective in removal of cells from ovarian cortex, SDS-Triton-Ammonium group showed less residual DNA content with higher cytobiocompatibility for follicles when compared with other groups. In addition, the scaffold made from ovarian tissues decellularized using SDS-Triton-Ammonium and sodium alginate is suggested as a potential 3D substrate for in vitro culture of follicles for fertility preservation. |
| first_indexed | 2024-12-17T05:41:51Z |
| format | Article |
| id | doaj.art-656673054de7418581d2dbcda8f3cf31 |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-12-17T05:41:51Z |
| publishDate | 2021-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-656673054de7418581d2dbcda8f3cf312022-12-21T22:01:25ZengBMCBMC Biotechnology1472-67502021-01-012111810.1186/s12896-020-00658-3Comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix-alginate scaffold for better follicle development in vitroHossein Nikniaz0Zahra Zandieh1Mohammad Nouri2Neda Daei-farshbaf3Reza Aflatoonian4Mazaher Gholipourmalekabadi5Seyed Behnamedin Jameie6Department of Anatomical sciences, School of Medicine, Iran University of Medical SciencesDepartment of Anatomical sciences, School of Medicine, Iran University of Medical SciencesDepartment of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical SciencesDepartment of Anatomical sciences, School of Medicine, Iran University of Medical SciencesDepartment of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECRCellular and Molecular Research Center, Iran University of Medical SciencesNeuroscience Research Center (NRC), Iran University of Medical SciencesAbstract Background Nowadays, the number of cancer survivors is significantly increasing as a result of efficient chemo/radio therapeutic treatments. Female cancer survivors may suffer from decreased fertility. In this regard, different fertility preservation techniques were developed. Artificial ovary is one of these methods suggested by several scientific groups. Decellularized ovarian cortex has been introduced as a scaffold in the field of human fertility preservation. This study was carried out to compare decellularization of the ovarian scaffold by various protocols and evaluate the follicle survival in extracellular matrix (ECM)-alginate scaffold. Results The micrographs of H&E and DAPI staining confirmed successful decellularization of the ovarian cortex in all experimental groups, but residual DNA content in SDS-Triton group was significantly higher than other groups (P < 0.05). SEM images demonstrated that complex fiber network and porosity structure were maintained in all groups. Furthermore, elastin and collagen fibers were observed in all groups after decellularization process. MTT test revealed higher cytobiocompatibility of the SDS-Triton-Ammonium and SDS-Triton decellularized scaffolds compared with SDS groups. Compared to the transferred follicles into the sodium alginate (81%), 85.9% of the transferred follicles into the decellularized scaffold were viable after 7 days of cultivation (P = 0.04). Conclusion Although all the decellularization procedures was effective in removal of cells from ovarian cortex, SDS-Triton-Ammonium group showed less residual DNA content with higher cytobiocompatibility for follicles when compared with other groups. In addition, the scaffold made from ovarian tissues decellularized using SDS-Triton-Ammonium and sodium alginate is suggested as a potential 3D substrate for in vitro culture of follicles for fertility preservation.https://doi.org/10.1186/s12896-020-00658-3DecellularizationScaffoldOvaryFertility preservation |
| spellingShingle | Hossein Nikniaz Zahra Zandieh Mohammad Nouri Neda Daei-farshbaf Reza Aflatoonian Mazaher Gholipourmalekabadi Seyed Behnamedin Jameie Comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix-alginate scaffold for better follicle development in vitro BMC Biotechnology Decellularization Scaffold Ovary Fertility preservation |
| title | Comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix-alginate scaffold for better follicle development in vitro |
| title_full | Comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix-alginate scaffold for better follicle development in vitro |
| title_fullStr | Comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix-alginate scaffold for better follicle development in vitro |
| title_full_unstemmed | Comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix-alginate scaffold for better follicle development in vitro |
| title_short | Comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix-alginate scaffold for better follicle development in vitro |
| title_sort | comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix alginate scaffold for better follicle development in vitro |
| topic | Decellularization Scaffold Ovary Fertility preservation |
| url | https://doi.org/10.1186/s12896-020-00658-3 |
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