Low Specificity but Dissimilar Mycorrhizal Communities Associating with Roots May Contribute to the Spatial Pattern of Four Co-Occurring <i>Habenaria</i> (Orchidaceae) Species
Fungi with orchid roots have been increasingly proven to play important roles in orchid growth, spatial distribution, and coexistence of natural communities. Here, we used 454 amplicon pyrosequencing with two different primer combinations to investigate the spatial variations in the community of OMF...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/1/665 |
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| author | Wenliu Zhang Jiangyun Gao Shicheng Shao Taiqiang Li |
| author_facet | Wenliu Zhang Jiangyun Gao Shicheng Shao Taiqiang Li |
| author_sort | Wenliu Zhang |
| collection | DOAJ |
| description | Fungi with orchid roots have been increasingly proven to play important roles in orchid growth, spatial distribution, and coexistence of natural communities. Here, we used 454 amplicon pyrosequencing with two different primer combinations to investigate the spatial variations in the community of OMF and endophytic fungi associates within the roots of four co-occurring <i>Habenaria</i> species. The results showed that all investigated <i>Habenaria</i> species were generalists and the different fungi communities may contribute to the spatial separation of the four <i>Habenaria</i> species. Firstly, the fungal OTUs identified in the roots of the four species overlapped but their presence differed amongst species and numerous distinct OMF families were unique to each species. Second, NMDS clustering showed samples clustered together based on associated species and PERMANOVA analyses indicated that fungi communities in the roots differed significantly between the <i>Habenaria</i> species, both for all endophytic fungi communities and for OMF communities. Third, the network structure of epiphytic fungi was highly specialized and modular but demonstrated lowly connected and anti-nested properties. However, it calls for more soil nutrition and soil fungal communities’ studies to elucidate the contribution of habitat-specific adaptations in general and mycorrhizal divergence. |
| first_indexed | 2024-03-11T09:58:53Z |
| format | Article |
| id | doaj.art-6569e70cf91e492488cdfd10ddb2f30a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:58:53Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6569e70cf91e492488cdfd10ddb2f30a2023-11-16T15:36:37ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0124166510.3390/ijms24010665Low Specificity but Dissimilar Mycorrhizal Communities Associating with Roots May Contribute to the Spatial Pattern of Four Co-Occurring <i>Habenaria</i> (Orchidaceae) SpeciesWenliu Zhang0Jiangyun Gao1Shicheng Shao2Taiqiang Li3Key Laboratory of Grassland Ecosystem of Ministry of Education, College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, ChinaInstitute of Biodiversity, School of Ecology and Environmental Science, Yunnan University, Kunming 650500, ChinaGardening and Horticulture Department, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, ChinaInstitute of Biodiversity, School of Ecology and Environmental Science, Yunnan University, Kunming 650500, ChinaFungi with orchid roots have been increasingly proven to play important roles in orchid growth, spatial distribution, and coexistence of natural communities. Here, we used 454 amplicon pyrosequencing with two different primer combinations to investigate the spatial variations in the community of OMF and endophytic fungi associates within the roots of four co-occurring <i>Habenaria</i> species. The results showed that all investigated <i>Habenaria</i> species were generalists and the different fungi communities may contribute to the spatial separation of the four <i>Habenaria</i> species. Firstly, the fungal OTUs identified in the roots of the four species overlapped but their presence differed amongst species and numerous distinct OMF families were unique to each species. Second, NMDS clustering showed samples clustered together based on associated species and PERMANOVA analyses indicated that fungi communities in the roots differed significantly between the <i>Habenaria</i> species, both for all endophytic fungi communities and for OMF communities. Third, the network structure of epiphytic fungi was highly specialized and modular but demonstrated lowly connected and anti-nested properties. However, it calls for more soil nutrition and soil fungal communities’ studies to elucidate the contribution of habitat-specific adaptations in general and mycorrhizal divergence.https://www.mdpi.com/1422-0067/24/1/665<i>Habenaria</i>orchid mycorrhizal fungi (OMF)endophytic fungifungi communitiesnetwork structureco-occurring |
| spellingShingle | Wenliu Zhang Jiangyun Gao Shicheng Shao Taiqiang Li Low Specificity but Dissimilar Mycorrhizal Communities Associating with Roots May Contribute to the Spatial Pattern of Four Co-Occurring <i>Habenaria</i> (Orchidaceae) Species International Journal of Molecular Sciences <i>Habenaria</i> orchid mycorrhizal fungi (OMF) endophytic fungi fungi communities network structure co-occurring |
| title | Low Specificity but Dissimilar Mycorrhizal Communities Associating with Roots May Contribute to the Spatial Pattern of Four Co-Occurring <i>Habenaria</i> (Orchidaceae) Species |
| title_full | Low Specificity but Dissimilar Mycorrhizal Communities Associating with Roots May Contribute to the Spatial Pattern of Four Co-Occurring <i>Habenaria</i> (Orchidaceae) Species |
| title_fullStr | Low Specificity but Dissimilar Mycorrhizal Communities Associating with Roots May Contribute to the Spatial Pattern of Four Co-Occurring <i>Habenaria</i> (Orchidaceae) Species |
| title_full_unstemmed | Low Specificity but Dissimilar Mycorrhizal Communities Associating with Roots May Contribute to the Spatial Pattern of Four Co-Occurring <i>Habenaria</i> (Orchidaceae) Species |
| title_short | Low Specificity but Dissimilar Mycorrhizal Communities Associating with Roots May Contribute to the Spatial Pattern of Four Co-Occurring <i>Habenaria</i> (Orchidaceae) Species |
| title_sort | low specificity but dissimilar mycorrhizal communities associating with roots may contribute to the spatial pattern of four co occurring i habenaria i orchidaceae species |
| topic | <i>Habenaria</i> orchid mycorrhizal fungi (OMF) endophytic fungi fungi communities network structure co-occurring |
| url | https://www.mdpi.com/1422-0067/24/1/665 |
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