Uncovering neglected subtypes and zoonotic transmission of Hepatitis E virus (HEV) in Brazil
Abstract Hepatitis E virus (HEV) circulation in humans and swine has been extensively studied in South America over the last two decades. Nevertheless, only 2.1% of reported HEV strains are available as complete genome sequences. Therefore, many clinical, epidemiological, and evolutionary aspects of...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-05-01
|
| Series: | Virology Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12985-023-02047-6 |
| _version_ | 1797832224342540288 |
|---|---|
| author | Debora Regina Lopes dos Santos Ricardo Durães-Carvalho Noemi Rovaris Gardinali Lais Ceschini Machado Vanessa Salete de Paula Gabriel da Luz Wallau Jaqueline Mendes de Oliveira Lindomar José Pena Marcelo Alves Pinto Laura Helena Vega Gonzalez Gil Edmilson Ferreira de Oliveira-Filho |
| author_facet | Debora Regina Lopes dos Santos Ricardo Durães-Carvalho Noemi Rovaris Gardinali Lais Ceschini Machado Vanessa Salete de Paula Gabriel da Luz Wallau Jaqueline Mendes de Oliveira Lindomar José Pena Marcelo Alves Pinto Laura Helena Vega Gonzalez Gil Edmilson Ferreira de Oliveira-Filho |
| author_sort | Debora Regina Lopes dos Santos |
| collection | DOAJ |
| description | Abstract Hepatitis E virus (HEV) circulation in humans and swine has been extensively studied in South America over the last two decades. Nevertheless, only 2.1% of reported HEV strains are available as complete genome sequences. Therefore, many clinical, epidemiological, and evolutionary aspects of circulating HEV in the continent still need to be clarified. Here, we conducted a retrospective evolutionary analysis of one human case and six swine HEV strains previously reported in northeastern, southern, and southeastern Brazil. We obtained two complete and four nearly complete genomic sequences. Evolutionary analysis comparing the whole genomic and capsid gene sequences revealed high genetic variability. This included the circulation of at least one unrecognized unique South American subtype. Our results corroborate that sequencing the whole capsid gene could be used as an alternative for HEV subtype assignment in the absence of complete genomic sequences. Moreover, our results substantiate the evidence for zoonotic transmission by comparing a larger genomic fragment recovered from the sample of the autochthonous human hepatitis E case. Further studies should continuously investigate HEV genetic diversity and zoonotic transmission of HEV in South America. |
| first_indexed | 2024-04-09T14:04:22Z |
| format | Article |
| id | doaj.art-656e236eacc849f6a0c180139f8cecad |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-09T14:04:22Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-656e236eacc849f6a0c180139f8cecad2023-05-07T11:05:24ZengBMCVirology Journal1743-422X2023-05-012011710.1186/s12985-023-02047-6Uncovering neglected subtypes and zoonotic transmission of Hepatitis E virus (HEV) in BrazilDebora Regina Lopes dos Santos0Ricardo Durães-Carvalho1Noemi Rovaris Gardinali2Lais Ceschini Machado3Vanessa Salete de Paula4Gabriel da Luz Wallau5Jaqueline Mendes de Oliveira6Lindomar José Pena7Marcelo Alves Pinto8Laura Helena Vega Gonzalez Gil9Edmilson Ferreira de Oliveira-Filho10Department of Virology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation (IAM- FIOCRUZ)São Paulo School of Medicine, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP)Laboratório de Desenvolvimento Tecnológico em Virologia (LADTV) , Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (Fiocruz)Department of Entomology and Bioinformatic Core, Oswaldo Cruz Foundation-FiocruzLaboratory of Molecular Virology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation (IOC- FIOCRUZ)Department of Entomology and Bioinformatic Core, Oswaldo Cruz Foundation-FiocruzLaboratório de Desenvolvimento Tecnológico em Virologia (LADTV) , Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (Fiocruz)Department of Virology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation (IAM- FIOCRUZ)Laboratório de Desenvolvimento Tecnológico em Virologia (LADTV) , Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (Fiocruz)Department of Virology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation (IAM- FIOCRUZ)Department of Virology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation (IAM- FIOCRUZ)Abstract Hepatitis E virus (HEV) circulation in humans and swine has been extensively studied in South America over the last two decades. Nevertheless, only 2.1% of reported HEV strains are available as complete genome sequences. Therefore, many clinical, epidemiological, and evolutionary aspects of circulating HEV in the continent still need to be clarified. Here, we conducted a retrospective evolutionary analysis of one human case and six swine HEV strains previously reported in northeastern, southern, and southeastern Brazil. We obtained two complete and four nearly complete genomic sequences. Evolutionary analysis comparing the whole genomic and capsid gene sequences revealed high genetic variability. This included the circulation of at least one unrecognized unique South American subtype. Our results corroborate that sequencing the whole capsid gene could be used as an alternative for HEV subtype assignment in the absence of complete genomic sequences. Moreover, our results substantiate the evidence for zoonotic transmission by comparing a larger genomic fragment recovered from the sample of the autochthonous human hepatitis E case. Further studies should continuously investigate HEV genetic diversity and zoonotic transmission of HEV in South America.https://doi.org/10.1186/s12985-023-02047-6Hepatitis EHEVZoonotic transmissionGenetic divergenceSubtypes assignment |
| spellingShingle | Debora Regina Lopes dos Santos Ricardo Durães-Carvalho Noemi Rovaris Gardinali Lais Ceschini Machado Vanessa Salete de Paula Gabriel da Luz Wallau Jaqueline Mendes de Oliveira Lindomar José Pena Marcelo Alves Pinto Laura Helena Vega Gonzalez Gil Edmilson Ferreira de Oliveira-Filho Uncovering neglected subtypes and zoonotic transmission of Hepatitis E virus (HEV) in Brazil Virology Journal Hepatitis E HEV Zoonotic transmission Genetic divergence Subtypes assignment |
| title | Uncovering neglected subtypes and zoonotic transmission of Hepatitis E virus (HEV) in Brazil |
| title_full | Uncovering neglected subtypes and zoonotic transmission of Hepatitis E virus (HEV) in Brazil |
| title_fullStr | Uncovering neglected subtypes and zoonotic transmission of Hepatitis E virus (HEV) in Brazil |
| title_full_unstemmed | Uncovering neglected subtypes and zoonotic transmission of Hepatitis E virus (HEV) in Brazil |
| title_short | Uncovering neglected subtypes and zoonotic transmission of Hepatitis E virus (HEV) in Brazil |
| title_sort | uncovering neglected subtypes and zoonotic transmission of hepatitis e virus hev in brazil |
| topic | Hepatitis E HEV Zoonotic transmission Genetic divergence Subtypes assignment |
| url | https://doi.org/10.1186/s12985-023-02047-6 |
| work_keys_str_mv | AT deborareginalopesdossantos uncoveringneglectedsubtypesandzoonotictransmissionofhepatitisevirushevinbrazil AT ricardoduraescarvalho uncoveringneglectedsubtypesandzoonotictransmissionofhepatitisevirushevinbrazil AT noemirovarisgardinali uncoveringneglectedsubtypesandzoonotictransmissionofhepatitisevirushevinbrazil AT laisceschinimachado uncoveringneglectedsubtypesandzoonotictransmissionofhepatitisevirushevinbrazil AT vanessasaletedepaula uncoveringneglectedsubtypesandzoonotictransmissionofhepatitisevirushevinbrazil AT gabrieldaluzwallau uncoveringneglectedsubtypesandzoonotictransmissionofhepatitisevirushevinbrazil AT jaquelinemendesdeoliveira uncoveringneglectedsubtypesandzoonotictransmissionofhepatitisevirushevinbrazil AT lindomarjosepena uncoveringneglectedsubtypesandzoonotictransmissionofhepatitisevirushevinbrazil AT marceloalvespinto uncoveringneglectedsubtypesandzoonotictransmissionofhepatitisevirushevinbrazil AT laurahelenavegagonzalezgil uncoveringneglectedsubtypesandzoonotictransmissionofhepatitisevirushevinbrazil AT edmilsonferreiradeoliveirafilho uncoveringneglectedsubtypesandzoonotictransmissionofhepatitisevirushevinbrazil |