Phytochemical and Biological Investigation of Two Diplotaxis Species Growing in Tunisia: D. virgata & D. erucoides
A phytochemical investigation of Diplotaxis virgata D.C. and D. erucoides (L.) D.C. (Brassicaceae) offered to the isolation of two new flavonoids isorhamnetin-3-O-α-l-glucopyranoside (1) and rhamnetin-3,3ʹ-di-O-β-d-glucopyranoside (2), respectively. Their structures have been elucidated from the ext...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-10-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/20/10/18128 |
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| author | Nizar Ben Salah Hervé Casabianca Hichem Ben Jannet Sophie Chenavas Corinne Sanglar Aurélie Fildier Nabiha Bouzouita |
| author_facet | Nizar Ben Salah Hervé Casabianca Hichem Ben Jannet Sophie Chenavas Corinne Sanglar Aurélie Fildier Nabiha Bouzouita |
| author_sort | Nizar Ben Salah |
| collection | DOAJ |
| description | A phytochemical investigation of Diplotaxis virgata D.C. and D. erucoides (L.) D.C. (Brassicaceae) offered to the isolation of two new flavonoids isorhamnetin-3-O-α-l-glucopyranoside (1) and rhamnetin-3,3ʹ-di-O-β-d-glucopyranoside (2), respectively. Their structures have been elucidated from the extended spectroscopic methods, including 1D- and 2D-NMR, UV and mass spectrometry analysis and by comparison with literature data. The fatty acid composition of the hexane extracts of the two species was also investigated by using GC-MS. The antioxidant activity of ethanol, ethyl acetate, n-butanol extracts and the isolated compounds from the two species was evaluated using DPPH and ABTS+ scavenging assays. All the tested samples showed an efficient radical scavenging ability, with IC50 values ranging from 16–40 µg/mL for the DPPH and from 17–44 µg/mL for the ABTS+ assays. In addition, the antibacterial activity of the prepared extracts and compounds 1 and 2, determined by well diffusion agar method against two Gram positive and five Gram negative bacteria, was evaluated and the results showed significant effects against all strains used. |
| first_indexed | 2024-12-23T20:39:06Z |
| format | Article |
| id | doaj.art-656eba62aae04e058dcba8e5d63fc34b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-23T20:39:06Z |
| publishDate | 2015-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-656eba62aae04e058dcba8e5d63fc34b2022-12-21T17:31:58ZengMDPI AGMolecules1420-30492015-10-012010181281814310.3390/molecules201018128molecules201018128Phytochemical and Biological Investigation of Two Diplotaxis Species Growing in Tunisia: D. virgata & D. erucoidesNizar Ben Salah0Hervé Casabianca1Hichem Ben Jannet2Sophie Chenavas3Corinne Sanglar4Aurélie Fildier5Nabiha Bouzouita6Faculté des Sciences de Monastir, Université de Monastir, Avenue de l'Environnement, 5019 Monastir, TunisiaService Central d'analyse, Institut des Sciences Analytiques, Université de Lyon, UMR 5280 CNRS, UniversitéLaboratoire de Chimie Hétérocyclique, Produits Naturels et Réactivité, Equipe, Chimie Médicinale et Produits Naturels, Faculté des Sciences de Monastir, Université de Monastir, Avenue of the Environment, 5019 Monastir, TunisiaService Central d'analyse, Institut des Sciences Analytiques, Université de Lyon, UMR 5280 CNRS, UniversitéService Central d'analyse, Institut des Sciences Analytiques, Université de Lyon, UMR 5280 CNRS, UniversitéService Central d'analyse, Institut des Sciences Analytiques, Université de Lyon, UMR 5280 CNRS, UniversitéLaboratoire de Chimie Organique Structurale, Synthèse et Etude Physicochimique–Faculté des Sciences de Tunis, 2092 El Manar, TunisiaA phytochemical investigation of Diplotaxis virgata D.C. and D. erucoides (L.) D.C. (Brassicaceae) offered to the isolation of two new flavonoids isorhamnetin-3-O-α-l-glucopyranoside (1) and rhamnetin-3,3ʹ-di-O-β-d-glucopyranoside (2), respectively. Their structures have been elucidated from the extended spectroscopic methods, including 1D- and 2D-NMR, UV and mass spectrometry analysis and by comparison with literature data. The fatty acid composition of the hexane extracts of the two species was also investigated by using GC-MS. The antioxidant activity of ethanol, ethyl acetate, n-butanol extracts and the isolated compounds from the two species was evaluated using DPPH and ABTS+ scavenging assays. All the tested samples showed an efficient radical scavenging ability, with IC50 values ranging from 16–40 µg/mL for the DPPH and from 17–44 µg/mL for the ABTS+ assays. In addition, the antibacterial activity of the prepared extracts and compounds 1 and 2, determined by well diffusion agar method against two Gram positive and five Gram negative bacteria, was evaluated and the results showed significant effects against all strains used.http://www.mdpi.com/1420-3049/20/10/18128D. erucoides D. virgataflavonoidsfatty acidsNMRantioxidant activityantimicrobial activity |
| spellingShingle | Nizar Ben Salah Hervé Casabianca Hichem Ben Jannet Sophie Chenavas Corinne Sanglar Aurélie Fildier Nabiha Bouzouita Phytochemical and Biological Investigation of Two Diplotaxis Species Growing in Tunisia: D. virgata & D. erucoides Molecules D. erucoides D. virgata flavonoids fatty acids NMR antioxidant activity antimicrobial activity |
| title | Phytochemical and Biological Investigation of Two Diplotaxis Species Growing in Tunisia: D. virgata & D. erucoides |
| title_full | Phytochemical and Biological Investigation of Two Diplotaxis Species Growing in Tunisia: D. virgata & D. erucoides |
| title_fullStr | Phytochemical and Biological Investigation of Two Diplotaxis Species Growing in Tunisia: D. virgata & D. erucoides |
| title_full_unstemmed | Phytochemical and Biological Investigation of Two Diplotaxis Species Growing in Tunisia: D. virgata & D. erucoides |
| title_short | Phytochemical and Biological Investigation of Two Diplotaxis Species Growing in Tunisia: D. virgata & D. erucoides |
| title_sort | phytochemical and biological investigation of two diplotaxis species growing in tunisia d virgata amp d erucoides |
| topic | D. erucoides D. virgata flavonoids fatty acids NMR antioxidant activity antimicrobial activity |
| url | http://www.mdpi.com/1420-3049/20/10/18128 |
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