Pretreatment of NaCl enhances the drought resistance of cotton by regulating the biosynthesis of carotenoids and abscisic acid
Drought stress is one of the abiotic stresses that limits crop production and greatly affects crop yield. Enhancement of plant stress resistance by NaCl pretreatment has been reported, but the mechanism by which NaCl pretreatment activates cotton stress resistance remains unclear. In this study, upl...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Environmental Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fenvs.2022.998141/full |
| _version_ | 1811216156829155328 |
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| author | Kesong Ni Maohua Dai Xuke Lu Yuexin Zhang Yapeng Fan Nan Xu Xixian Feng Hui Huang Jing Wang Cun Rui Hong Zhang Yuqian Lei Xiaoyu Liu Tiantian Jiang Mingge Han Liangqing Sun Xiugui Chen Delong Wang Junjuan Wang Shuai Wang Chao Chen Lixue Guo Lanjie Zhao Wuwei Ye |
| author_facet | Kesong Ni Maohua Dai Xuke Lu Yuexin Zhang Yapeng Fan Nan Xu Xixian Feng Hui Huang Jing Wang Cun Rui Hong Zhang Yuqian Lei Xiaoyu Liu Tiantian Jiang Mingge Han Liangqing Sun Xiugui Chen Delong Wang Junjuan Wang Shuai Wang Chao Chen Lixue Guo Lanjie Zhao Wuwei Ye |
| author_sort | Kesong Ni |
| collection | DOAJ |
| description | Drought stress is one of the abiotic stresses that limits crop production and greatly affects crop yield. Enhancement of plant stress resistance by NaCl pretreatment has been reported, but the mechanism by which NaCl pretreatment activates cotton stress resistance remains unclear. In this study, upland cotton (Gossypium hirsutum cv H177) was used as the material to conducted the treatments with three replications: 0 Mm NaCl + 0% PEG6000 (Polyethylene glycol), 0 mM NaCl + 15% PEG6000, 50 mM NaCl + 15% PEG6000 to explore the molecular mechanism by which NaCl improves the drought tolerance of cotton. The results showed that pretreatment with 50 mM NaCl could alleviate the adverse effects of PEG on cotton seeds while promoting the elongation of root length. RNA-seq showed that NaCl specifically induced the expression of carotenoid-related genes. By silencing the upstream gene GHLUT2 of lutein synthesis, it was found that the chlorophyll of silenced plants decreased, and leaf wilting was more sensitive to drought. We found that NaCl enhanced the drought resistance of cotton by regulating genes related to the carotenoid and abscisic acid downstream synthesis pathways. This study provides a new reference for the study of drought resistance in cotton and a theoretical basis for the molecular breeding of cotton. |
| first_indexed | 2024-04-12T06:33:44Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2296-665X |
| language | English |
| last_indexed | 2024-04-12T06:33:44Z |
| publishDate | 2022-11-01 |
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| series | Frontiers in Environmental Science |
| spelling | doaj.art-657034820f3e4bc698512cf7544a46782022-12-22T03:43:56ZengFrontiers Media S.A.Frontiers in Environmental Science2296-665X2022-11-011010.3389/fenvs.2022.998141998141Pretreatment of NaCl enhances the drought resistance of cotton by regulating the biosynthesis of carotenoids and abscisic acidKesong Ni0Maohua Dai1Xuke Lu2Yuexin Zhang3Yapeng Fan4Nan Xu5Xixian Feng6Hui Huang7Jing Wang8Cun Rui9Hong Zhang10Yuqian Lei11Xiaoyu Liu12Tiantian Jiang13Mingge Han14Liangqing Sun15Xiugui Chen16Delong Wang17Junjuan Wang18Shuai Wang19Chao Chen20Lixue Guo21Lanjie Zhao22Wuwei Ye23State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaHebei Academy of Agricultural and Forestry Sciences/Hebei Key Laboratory of Crops Drought Resistance, Dryland Farming Institute, Hengshui, Hebei, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaState Key Laboratory of Cotton Biology, School of Agricultural Sciences, Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Zhengzhou University, Anyang, Henan, ChinaDrought stress is one of the abiotic stresses that limits crop production and greatly affects crop yield. Enhancement of plant stress resistance by NaCl pretreatment has been reported, but the mechanism by which NaCl pretreatment activates cotton stress resistance remains unclear. In this study, upland cotton (Gossypium hirsutum cv H177) was used as the material to conducted the treatments with three replications: 0 Mm NaCl + 0% PEG6000 (Polyethylene glycol), 0 mM NaCl + 15% PEG6000, 50 mM NaCl + 15% PEG6000 to explore the molecular mechanism by which NaCl improves the drought tolerance of cotton. The results showed that pretreatment with 50 mM NaCl could alleviate the adverse effects of PEG on cotton seeds while promoting the elongation of root length. RNA-seq showed that NaCl specifically induced the expression of carotenoid-related genes. By silencing the upstream gene GHLUT2 of lutein synthesis, it was found that the chlorophyll of silenced plants decreased, and leaf wilting was more sensitive to drought. We found that NaCl enhanced the drought resistance of cotton by regulating genes related to the carotenoid and abscisic acid downstream synthesis pathways. This study provides a new reference for the study of drought resistance in cotton and a theoretical basis for the molecular breeding of cotton.https://www.frontiersin.org/articles/10.3389/fenvs.2022.998141/fullNaCl pretreatmentcarotenoid metabolismcottondrought resistanceabscisic acid |
| spellingShingle | Kesong Ni Maohua Dai Xuke Lu Yuexin Zhang Yapeng Fan Nan Xu Xixian Feng Hui Huang Jing Wang Cun Rui Hong Zhang Yuqian Lei Xiaoyu Liu Tiantian Jiang Mingge Han Liangqing Sun Xiugui Chen Delong Wang Junjuan Wang Shuai Wang Chao Chen Lixue Guo Lanjie Zhao Wuwei Ye Pretreatment of NaCl enhances the drought resistance of cotton by regulating the biosynthesis of carotenoids and abscisic acid Frontiers in Environmental Science NaCl pretreatment carotenoid metabolism cotton drought resistance abscisic acid |
| title | Pretreatment of NaCl enhances the drought resistance of cotton by regulating the biosynthesis of carotenoids and abscisic acid |
| title_full | Pretreatment of NaCl enhances the drought resistance of cotton by regulating the biosynthesis of carotenoids and abscisic acid |
| title_fullStr | Pretreatment of NaCl enhances the drought resistance of cotton by regulating the biosynthesis of carotenoids and abscisic acid |
| title_full_unstemmed | Pretreatment of NaCl enhances the drought resistance of cotton by regulating the biosynthesis of carotenoids and abscisic acid |
| title_short | Pretreatment of NaCl enhances the drought resistance of cotton by regulating the biosynthesis of carotenoids and abscisic acid |
| title_sort | pretreatment of nacl enhances the drought resistance of cotton by regulating the biosynthesis of carotenoids and abscisic acid |
| topic | NaCl pretreatment carotenoid metabolism cotton drought resistance abscisic acid |
| url | https://www.frontiersin.org/articles/10.3389/fenvs.2022.998141/full |
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