Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing
Background: Among various bio-informative molecules transferred by exosomes between cells, micro RNAs (miRNAs), which remain remarkably stable even after freeze-and-thaw cycles, are excellent candidates for potential biomarkers for coronary artery disease (CAD). Methods: Blood samples were collected...
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MDPI AG
2021-12-01
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| Online Access: | https://www.mdpi.com/2073-4409/11/1/98 |
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| author | Sheng-Nan Chang Jien-Jiun Chen Jo-Hsuan Wu Yao-Te Chung Jin-Wun Chen Chu-Hsuan Chiu Chia-Ju Liu Meng-Tsun Liu Yi-Cheng Chang Chin Li Jou-Wei Lin Juey-Jen Hwang Wen-Pin Lien |
| author_facet | Sheng-Nan Chang Jien-Jiun Chen Jo-Hsuan Wu Yao-Te Chung Jin-Wun Chen Chu-Hsuan Chiu Chia-Ju Liu Meng-Tsun Liu Yi-Cheng Chang Chin Li Jou-Wei Lin Juey-Jen Hwang Wen-Pin Lien |
| author_sort | Sheng-Nan Chang |
| collection | DOAJ |
| description | Background: Among various bio-informative molecules transferred by exosomes between cells, micro RNAs (miRNAs), which remain remarkably stable even after freeze-and-thaw cycles, are excellent candidates for potential biomarkers for coronary artery disease (CAD). Methods: Blood samples were collected from the coronary arteries of 214 patients diagnosed with three-vessel CAD and 140 without CAD. After precipitation extraction, the amounts of exosomes were found to decrease with increased age and three-vessel CAD. Next-generation sequencing was performed to further explore the possible relationship between exosomal miRNAs and CAD. Results: Eight exosomal miRNAs showed altered expression associated with CAD. The up-regulated miRNAs in CAD were miRNA-382-3p, miRNA-432-5p, miRNA-200a-3p, and miRNA-3613-3p. The down-regulated miRNAs were miRNA-125a-5p, miRNA-185-5p, miRNA-151a-3p, and miRNA-328-3p. Conclusion: We successfully demonstrated particular exosomal miRNAs that may serve as future biomarkers for CAD. |
| first_indexed | 2024-03-10T03:46:21Z |
| format | Article |
| id | doaj.art-65704abdeec64dde99d12d912bd9a6e3 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T03:46:21Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-65704abdeec64dde99d12d912bd9a6e32023-11-23T11:20:09ZengMDPI AGCells2073-44092021-12-011119810.3390/cells11010098Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation SequencingSheng-Nan Chang0Jien-Jiun Chen1Jo-Hsuan Wu2Yao-Te Chung3Jin-Wun Chen4Chu-Hsuan Chiu5Chia-Ju Liu6Meng-Tsun Liu7Yi-Cheng Chang8Chin Li9Jou-Wei Lin10Juey-Jen Hwang11Wen-Pin Lien12Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, TaiwanDivision of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, TaiwanShiley Eye Institute and Viterbi Family Department of Ophthalmology, University of California, San Diego, CA 92093, USADivision of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, TaiwanDivision of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, TaiwanGraduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, TaiwanDepartment of Nuclear Medicine, National Taiwan University Hospital, Taipei 100, TaiwanDivision of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, TaiwanGraduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, TaiwanDepartment of Biomedical Sciences, National Chung-Cheng University, Chia-Yi 62102, TaiwanDivision of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, TaiwanDivision of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, TaiwanDepartment of Internal Medicine, National Taiwan University Hospital, Taipei 100, TaiwanBackground: Among various bio-informative molecules transferred by exosomes between cells, micro RNAs (miRNAs), which remain remarkably stable even after freeze-and-thaw cycles, are excellent candidates for potential biomarkers for coronary artery disease (CAD). Methods: Blood samples were collected from the coronary arteries of 214 patients diagnosed with three-vessel CAD and 140 without CAD. After precipitation extraction, the amounts of exosomes were found to decrease with increased age and three-vessel CAD. Next-generation sequencing was performed to further explore the possible relationship between exosomal miRNAs and CAD. Results: Eight exosomal miRNAs showed altered expression associated with CAD. The up-regulated miRNAs in CAD were miRNA-382-3p, miRNA-432-5p, miRNA-200a-3p, and miRNA-3613-3p. The down-regulated miRNAs were miRNA-125a-5p, miRNA-185-5p, miRNA-151a-3p, and miRNA-328-3p. Conclusion: We successfully demonstrated particular exosomal miRNAs that may serve as future biomarkers for CAD.https://www.mdpi.com/2073-4409/11/1/98coronary artery diseaseexosomesmicro RNAsnext-generation sequencing |
| spellingShingle | Sheng-Nan Chang Jien-Jiun Chen Jo-Hsuan Wu Yao-Te Chung Jin-Wun Chen Chu-Hsuan Chiu Chia-Ju Liu Meng-Tsun Liu Yi-Cheng Chang Chin Li Jou-Wei Lin Juey-Jen Hwang Wen-Pin Lien Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing Cells coronary artery disease exosomes micro RNAs next-generation sequencing |
| title | Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing |
| title_full | Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing |
| title_fullStr | Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing |
| title_full_unstemmed | Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing |
| title_short | Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing |
| title_sort | association between exosomal mirnas and coronary artery disease by next generation sequencing |
| topic | coronary artery disease exosomes micro RNAs next-generation sequencing |
| url | https://www.mdpi.com/2073-4409/11/1/98 |
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