Identification of HLA Class I Misreads/Dropouts Using Serological Typing, in Comparison with DNA-based Typing
Serology and DNA techniques are employed for Human Leukocyte Antigen (HLA) typing in different transplant centers. Results may not always correlate well and may need retyping with different technique. All the patients (with aplastic anemia, thalassemia, and immunodeficiency) and their donors, requir...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2016-10-01
|
| Series: | Iranian Journal of Allergy, Asthma and Immunology |
| Subjects: | |
| Online Access: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/784 |
| _version_ | 1811277686359719936 |
|---|---|
| author | Hamid Nawaz Tipu Muhammad Mukarram Bashir Muhammad Noman |
| author_facet | Hamid Nawaz Tipu Muhammad Mukarram Bashir Muhammad Noman |
| author_sort | Hamid Nawaz Tipu |
| collection | DOAJ |
| description | Serology and DNA techniques are employed for Human Leukocyte Antigen (HLA) typing in different transplant centers. Results may not always correlate well and may need retyping with different technique. All the patients (with aplastic anemia, thalassemia, and immunodeficiency) and their donors, requiring HLA typing for bone marrow transplant were enrolled in the study. Serological HLA typing was done by complement-dependent lymphocytotoxicity while DNA-based typing was done with sequence specific primers (SSP). Serology identified 167 HLA A and 165 HLA B antigens while SSP in same samples identified 181 HLA A and 184 HLA B alleles. A11 and B51 were the commonest antigens/alleles by both methods. There were a total of 21 misreads and 32 dropouts on serology, for both HLA A and B loci with HLA A32, B52 and B61 being the most ambiguous antigens. Inherent limitations of serological techniques warrant careful interpretation or use of DNA-based methods for resolution of ambiguous typing. |
| first_indexed | 2024-04-13T00:21:13Z |
| format | Article |
| id | doaj.art-6572a5afd2ad47c8935d29eda89b73ee |
| institution | Directory Open Access Journal |
| issn | 1735-1502 1735-5249 |
| language | English |
| last_indexed | 2024-04-13T00:21:13Z |
| publishDate | 2016-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Allergy, Asthma and Immunology |
| spelling | doaj.art-6572a5afd2ad47c8935d29eda89b73ee2022-12-22T03:10:46ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492016-10-01155643Identification of HLA Class I Misreads/Dropouts Using Serological Typing, in Comparison with DNA-based TypingHamid Nawaz Tipu0Muhammad Mukarram Bashir1Muhammad Noman2Department of Immunology, Armed Forces Institute of Pathology, Rawalpindi, PakistanDepartment of Immunology, Armed Forces Institute of Pathology, Rawalpindi, PakistanDepartment of Immunology, Armed Forces Institute of Pathology, Rawalpindi, PakistanSerology and DNA techniques are employed for Human Leukocyte Antigen (HLA) typing in different transplant centers. Results may not always correlate well and may need retyping with different technique. All the patients (with aplastic anemia, thalassemia, and immunodeficiency) and their donors, requiring HLA typing for bone marrow transplant were enrolled in the study. Serological HLA typing was done by complement-dependent lymphocytotoxicity while DNA-based typing was done with sequence specific primers (SSP). Serology identified 167 HLA A and 165 HLA B antigens while SSP in same samples identified 181 HLA A and 184 HLA B alleles. A11 and B51 were the commonest antigens/alleles by both methods. There were a total of 21 misreads and 32 dropouts on serology, for both HLA A and B loci with HLA A32, B52 and B61 being the most ambiguous antigens. Inherent limitations of serological techniques warrant careful interpretation or use of DNA-based methods for resolution of ambiguous typing.https://ijaai.tums.ac.ir/index.php/ijaai/article/view/784Ambiguous antigensComplement dependent lymphocytotoxicity (CDC)DNA based HLA typingHuman leukocyte antigen typingSequence specific primers (SSP) |
| spellingShingle | Hamid Nawaz Tipu Muhammad Mukarram Bashir Muhammad Noman Identification of HLA Class I Misreads/Dropouts Using Serological Typing, in Comparison with DNA-based Typing Iranian Journal of Allergy, Asthma and Immunology Ambiguous antigens Complement dependent lymphocytotoxicity (CDC) DNA based HLA typing Human leukocyte antigen typing Sequence specific primers (SSP) |
| title | Identification of HLA Class I Misreads/Dropouts Using Serological Typing, in Comparison with DNA-based Typing |
| title_full | Identification of HLA Class I Misreads/Dropouts Using Serological Typing, in Comparison with DNA-based Typing |
| title_fullStr | Identification of HLA Class I Misreads/Dropouts Using Serological Typing, in Comparison with DNA-based Typing |
| title_full_unstemmed | Identification of HLA Class I Misreads/Dropouts Using Serological Typing, in Comparison with DNA-based Typing |
| title_short | Identification of HLA Class I Misreads/Dropouts Using Serological Typing, in Comparison with DNA-based Typing |
| title_sort | identification of hla class i misreads dropouts using serological typing in comparison with dna based typing |
| topic | Ambiguous antigens Complement dependent lymphocytotoxicity (CDC) DNA based HLA typing Human leukocyte antigen typing Sequence specific primers (SSP) |
| url | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/784 |
| work_keys_str_mv | AT hamidnawaztipu identificationofhlaclassimisreadsdropoutsusingserologicaltypingincomparisonwithdnabasedtyping AT muhammadmukarrambashir identificationofhlaclassimisreadsdropoutsusingserologicaltypingincomparisonwithdnabasedtyping AT muhammadnoman identificationofhlaclassimisreadsdropoutsusingserologicaltypingincomparisonwithdnabasedtyping |