Periodontitis induced by orthodontic wire ligature drives oral microflora dysbiosis and aggravates alveolar bone loss in an improved murine model
AimTo assess the contribution of polymicrobial disruption of host homeostasis to periodontitis progression in orthodontic wire ligation murine model.MethodsOrthodontic wire rings were inserted between the first and second molars of mice for 18 days for the orthodontic wire ligation mouse model, and...
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.875091/full |
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| author | Rongshuang Ai Dingyi Li Luyao Shi Xiaonan Zhang Zhiqiang Ding Yiting Zhu Yiting Zhu Yujuan He |
| author_facet | Rongshuang Ai Dingyi Li Luyao Shi Xiaonan Zhang Zhiqiang Ding Yiting Zhu Yiting Zhu Yujuan He |
| author_sort | Rongshuang Ai |
| collection | DOAJ |
| description | AimTo assess the contribution of polymicrobial disruption of host homeostasis to periodontitis progression in orthodontic wire ligation murine model.MethodsOrthodontic wire rings were inserted between the first and second molars of mice for 18 days for the orthodontic wire ligation mouse model, and Pg injection model and Pg-LPS injection model were used as controls. Alveolar bone loss and periodontal inflammation were analyzed by micro-CT, histological staining and qRT-PCR. Further, pyrosequencing of 16S rRNA gene amplicon was used to analyze the development of oral microorganism dysbiosis in the mice.ResultsMicro-CT, TRAP staining and qRT-PCR showed that orthodontic wire ligation model led to more severe alveolar bone loss than Pg and Pg-LPS models.H&E staining and qRT-PCR demonstrated that stronger inflammatory response was induced by the orthodontic wire treatment compared to the other models. In addition, pyrosequencing of 16S rRNA gene amplicons revealed that the composition of oral microbiota presented a transition as the disease progressed and significant differences emerged in oral microbiota communities between orthodontic ligature mice and healthy controls. Furthermore, antibiotic treatment decreased both inflammation and alveolar bone loss in response to microbial community dysbiosis. However, no significant difference in bacterial community composition was observed in Pg and Pg-LPS models.ConclusionsOrthodontic wire ligation drove oral microbial community transitions that mimicked polymicrobial communities characterized by polymicrobial synergy and dysbiosis. Our improved model is suitable for further study of pathogenesis of periodontitis and exploration of corresponding treatment strategies. |
| first_indexed | 2024-04-11T11:46:35Z |
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| id | doaj.art-6573759e6e2f4477b9ec82aceaaec5ea |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T11:46:35Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6573759e6e2f4477b9ec82aceaaec5ea2022-12-22T04:25:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.875091875091Periodontitis induced by orthodontic wire ligature drives oral microflora dysbiosis and aggravates alveolar bone loss in an improved murine modelRongshuang Ai0Dingyi Li1Luyao Shi2Xiaonan Zhang3Zhiqiang Ding4Yiting Zhu5Yiting Zhu6Yujuan He7Key Laboratory of Diagnostic Medicine (Ministry of Education), Department of Laboratory Medicine, Chongqing Medical University, Chongqing, ChinaKey Laboratory of Diagnostic Medicine (Ministry of Education), Department of Laboratory Medicine, Chongqing Medical University, Chongqing, ChinaKey Laboratory of Diagnostic Medicine (Ministry of Education), Department of Laboratory Medicine, Chongqing Medical University, Chongqing, ChinaCollege of Stomatology, Chongqing Medical University, Chongqing, ChinaSchool of Computer Science, Chongqing Institute of Engineering, Chongqing, ChinaKey Laboratory of Diagnostic Medicine (Ministry of Education), Department of Laboratory Medicine, Chongqing Medical University, Chongqing, ChinaDepartment of Laboratory Medicine, Chongqing University Three Gorges Hospital, Chongqing, ChinaKey Laboratory of Diagnostic Medicine (Ministry of Education), Department of Laboratory Medicine, Chongqing Medical University, Chongqing, ChinaAimTo assess the contribution of polymicrobial disruption of host homeostasis to periodontitis progression in orthodontic wire ligation murine model.MethodsOrthodontic wire rings were inserted between the first and second molars of mice for 18 days for the orthodontic wire ligation mouse model, and Pg injection model and Pg-LPS injection model were used as controls. Alveolar bone loss and periodontal inflammation were analyzed by micro-CT, histological staining and qRT-PCR. Further, pyrosequencing of 16S rRNA gene amplicon was used to analyze the development of oral microorganism dysbiosis in the mice.ResultsMicro-CT, TRAP staining and qRT-PCR showed that orthodontic wire ligation model led to more severe alveolar bone loss than Pg and Pg-LPS models.H&E staining and qRT-PCR demonstrated that stronger inflammatory response was induced by the orthodontic wire treatment compared to the other models. In addition, pyrosequencing of 16S rRNA gene amplicons revealed that the composition of oral microbiota presented a transition as the disease progressed and significant differences emerged in oral microbiota communities between orthodontic ligature mice and healthy controls. Furthermore, antibiotic treatment decreased both inflammation and alveolar bone loss in response to microbial community dysbiosis. However, no significant difference in bacterial community composition was observed in Pg and Pg-LPS models.ConclusionsOrthodontic wire ligation drove oral microbial community transitions that mimicked polymicrobial communities characterized by polymicrobial synergy and dysbiosis. Our improved model is suitable for further study of pathogenesis of periodontitis and exploration of corresponding treatment strategies.https://www.frontiersin.org/articles/10.3389/fmicb.2022.875091/fullorthodontic wireligationinflammationalveolar bone loss16S rRNAmicrobiota dysbiosis |
| spellingShingle | Rongshuang Ai Dingyi Li Luyao Shi Xiaonan Zhang Zhiqiang Ding Yiting Zhu Yiting Zhu Yujuan He Periodontitis induced by orthodontic wire ligature drives oral microflora dysbiosis and aggravates alveolar bone loss in an improved murine model Frontiers in Microbiology orthodontic wire ligation inflammation alveolar bone loss 16S rRNA microbiota dysbiosis |
| title | Periodontitis induced by orthodontic wire ligature drives oral microflora dysbiosis and aggravates alveolar bone loss in an improved murine model |
| title_full | Periodontitis induced by orthodontic wire ligature drives oral microflora dysbiosis and aggravates alveolar bone loss in an improved murine model |
| title_fullStr | Periodontitis induced by orthodontic wire ligature drives oral microflora dysbiosis and aggravates alveolar bone loss in an improved murine model |
| title_full_unstemmed | Periodontitis induced by orthodontic wire ligature drives oral microflora dysbiosis and aggravates alveolar bone loss in an improved murine model |
| title_short | Periodontitis induced by orthodontic wire ligature drives oral microflora dysbiosis and aggravates alveolar bone loss in an improved murine model |
| title_sort | periodontitis induced by orthodontic wire ligature drives oral microflora dysbiosis and aggravates alveolar bone loss in an improved murine model |
| topic | orthodontic wire ligation inflammation alveolar bone loss 16S rRNA microbiota dysbiosis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.875091/full |
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