Inferring genetic structure when there is little: population genetics versus genomics of the threatened bat Miniopterus schreibersii across Europe
Abstract Despite their paramount importance in molecular ecology and conservation, genetic diversity and structure remain challenging to quantify with traditional genotyping methods. Next-generation sequencing holds great promises, but this has not been properly tested in highly mobile species. In t...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-27988-4 |
| _version_ | 1811175877927501824 |
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| author | Christophe Dufresnes Ludovic Dutoit Alan Brelsford Fardo Goldstein-Witsenburg Laura Clément Adria López-Baucells Jorge Palmeirim Igor Pavlinić Dino Scaravelli Martin Ševčík Philippe Christe Jérôme Goudet |
| author_facet | Christophe Dufresnes Ludovic Dutoit Alan Brelsford Fardo Goldstein-Witsenburg Laura Clément Adria López-Baucells Jorge Palmeirim Igor Pavlinić Dino Scaravelli Martin Ševčík Philippe Christe Jérôme Goudet |
| author_sort | Christophe Dufresnes |
| collection | DOAJ |
| description | Abstract Despite their paramount importance in molecular ecology and conservation, genetic diversity and structure remain challenging to quantify with traditional genotyping methods. Next-generation sequencing holds great promises, but this has not been properly tested in highly mobile species. In this article, we compared microsatellite and RAD-sequencing (RAD-seq) analyses to investigate population structure in the declining bent-winged bat (Miniopterus schreibersii) across Europe. Both markers retrieved general patterns of weak range-wide differentiation, little sex-biased dispersal, and strong isolation by distance that associated with significant genetic structure between the three Mediterranean Peninsulas, which could have acted as glacial refugia. Microsatellites proved uninformative in individual-based analyses, but the resolution offered by genomic SNPs illuminated on regional substructures within several countries, with colonies sharing migrators of distinct ancestry without admixture. This finding is consistent with a marked philopatry and spatial partitioning between mating and rearing grounds in the species, which was suspected from marked-recaptured data. Our study advocates that genomic data are necessary to properly unveil the genetic footprints left by biogeographic processes and social organization in long-distant flyers, which are otherwise rapidly blurred by their high levels of gene flow. |
| first_indexed | 2024-04-10T19:43:05Z |
| format | Article |
| id | doaj.art-6574a42520b24ded8b7b6501dbaaf023 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T19:43:05Z |
| publishDate | 2023-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6574a42520b24ded8b7b6501dbaaf0232023-01-29T12:11:40ZengNature PortfolioScientific Reports2045-23222023-01-0113111410.1038/s41598-023-27988-4Inferring genetic structure when there is little: population genetics versus genomics of the threatened bat Miniopterus schreibersii across EuropeChristophe Dufresnes0Ludovic Dutoit1Alan Brelsford2Fardo Goldstein-Witsenburg3Laura Clément4Adria López-Baucells5Jorge Palmeirim6Igor Pavlinić7Dino Scaravelli8Martin Ševčík9Philippe Christe10Jérôme Goudet11Laboratory for Amphibian Systematic and Evolutionary Research, College of Biology and the Environment, Nanjing Forestry UniversityDepartment of Ecology and Evolution, University of LausanneDepartment of Evolution, Ecology, and Organismal Biology, University of California RiversideDepartment of Ecology and Evolution, University of LausanneDepartment of Ecology and Evolution, University of LausanneBat Research Area, Granollers Museum of Natural SciencesDepartment of Animal Biology, Centre for Ecology, Evolution and Environmental Change – cE3c, University of LisbonDepartment of Zoology, Croatian Natural History MuseumDepartment of Biological, Geological, and Environmental Sciences, University of BolognaDepartment of Zoology, Faculty of Science, Charles University in PragueDepartment of Ecology and Evolution, University of LausanneDepartment of Ecology and Evolution, University of LausanneAbstract Despite their paramount importance in molecular ecology and conservation, genetic diversity and structure remain challenging to quantify with traditional genotyping methods. Next-generation sequencing holds great promises, but this has not been properly tested in highly mobile species. In this article, we compared microsatellite and RAD-sequencing (RAD-seq) analyses to investigate population structure in the declining bent-winged bat (Miniopterus schreibersii) across Europe. Both markers retrieved general patterns of weak range-wide differentiation, little sex-biased dispersal, and strong isolation by distance that associated with significant genetic structure between the three Mediterranean Peninsulas, which could have acted as glacial refugia. Microsatellites proved uninformative in individual-based analyses, but the resolution offered by genomic SNPs illuminated on regional substructures within several countries, with colonies sharing migrators of distinct ancestry without admixture. This finding is consistent with a marked philopatry and spatial partitioning between mating and rearing grounds in the species, which was suspected from marked-recaptured data. Our study advocates that genomic data are necessary to properly unveil the genetic footprints left by biogeographic processes and social organization in long-distant flyers, which are otherwise rapidly blurred by their high levels of gene flow.https://doi.org/10.1038/s41598-023-27988-4 |
| spellingShingle | Christophe Dufresnes Ludovic Dutoit Alan Brelsford Fardo Goldstein-Witsenburg Laura Clément Adria López-Baucells Jorge Palmeirim Igor Pavlinić Dino Scaravelli Martin Ševčík Philippe Christe Jérôme Goudet Inferring genetic structure when there is little: population genetics versus genomics of the threatened bat Miniopterus schreibersii across Europe Scientific Reports |
| title | Inferring genetic structure when there is little: population genetics versus genomics of the threatened bat Miniopterus schreibersii across Europe |
| title_full | Inferring genetic structure when there is little: population genetics versus genomics of the threatened bat Miniopterus schreibersii across Europe |
| title_fullStr | Inferring genetic structure when there is little: population genetics versus genomics of the threatened bat Miniopterus schreibersii across Europe |
| title_full_unstemmed | Inferring genetic structure when there is little: population genetics versus genomics of the threatened bat Miniopterus schreibersii across Europe |
| title_short | Inferring genetic structure when there is little: population genetics versus genomics of the threatened bat Miniopterus schreibersii across Europe |
| title_sort | inferring genetic structure when there is little population genetics versus genomics of the threatened bat miniopterus schreibersii across europe |
| url | https://doi.org/10.1038/s41598-023-27988-4 |
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