The analysis of virulence factors and antibiotic resistance between Helicobacter pylori strains isolated from gastric antrum and body
Abstract Background Individuals can be infected with multiple strains of Helicobacter pylori. However, the differences among co-infecting strains have not been well analyzed yet. This study aimed to investigate whether the virulence factors and antibiotic resistance patterns of H. pylori differ betw...
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| Format: | Article |
| Language: | English |
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BMC
2019-08-01
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| Series: | BMC Gastroenterology |
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| Online Access: | http://link.springer.com/article/10.1186/s12876-019-1062-5 |
| _version_ | 1818060013979041792 |
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| author | Ji Won Seo Jae Yong Park Tae-Seop Shin Jae Gyu Kim |
| author_facet | Ji Won Seo Jae Yong Park Tae-Seop Shin Jae Gyu Kim |
| author_sort | Ji Won Seo |
| collection | DOAJ |
| description | Abstract Background Individuals can be infected with multiple strains of Helicobacter pylori. However, the differences among co-infecting strains have not been well analyzed yet. This study aimed to investigate whether the virulence factors and antibiotic resistance patterns of H. pylori differ between strains isolated from different locations of the stomach in the same patient. Methods H. pylori isolates were obtained from the antrum and body of the stomach. Genetic differences were examined by random amplified polymorphic DNA (RAPD) fingerprinting. Antibiotic resistance was assessed using the agar dilution method. Virulence factors were identified by polymerase chain reaction and DNA sequencing. Results Among 80 patients, co-infection by two H. pylori strains was detected in 10 patients. Among the 10 pairs of H. pylori strains, differences in antibiotic resistance patterns were detected in 7 pairs (clarithromycin, 1 patient; quinolone, 3 patients; metronidazole, 4 patients) and differences in virulence factors were detected in 5 pairs. The cagA virulence gene was detected in all 10 patients, and 2 patients had H. pylori strains with different EPIYA motifs. Differences in vacA genotypes were detected in 4 patients. Conclusions Co-infection by two H. pylori strains was confirmed by RAPD fingerprinting. Frequently, two H. pylori strains obtained from a single host differed in their virulence factors and antibiotic resistance patterns. Co-infection by multiple H. pylori strains could undermine the success of eradication therapy and should be considered when interpreting the results of antimicrobial susceptibility tests. |
| first_indexed | 2024-12-10T13:25:40Z |
| format | Article |
| id | doaj.art-657589710bd44e17a3c0f04385974955 |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-12-10T13:25:40Z |
| publishDate | 2019-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-657589710bd44e17a3c0f043859749552022-12-22T01:47:11ZengBMCBMC Gastroenterology1471-230X2019-08-011911810.1186/s12876-019-1062-5The analysis of virulence factors and antibiotic resistance between Helicobacter pylori strains isolated from gastric antrum and bodyJi Won Seo0Jae Yong Park1Tae-Seop Shin2Jae Gyu Kim3Department of Biomedical Science, Chung-Ang University College of MedicineDepartment of Internal Medicine, Chung-Ang University HospitalResearch Institute, Chung-Ang UniversityDepartment of Internal Medicine, Chung-Ang University College of MedicineAbstract Background Individuals can be infected with multiple strains of Helicobacter pylori. However, the differences among co-infecting strains have not been well analyzed yet. This study aimed to investigate whether the virulence factors and antibiotic resistance patterns of H. pylori differ between strains isolated from different locations of the stomach in the same patient. Methods H. pylori isolates were obtained from the antrum and body of the stomach. Genetic differences were examined by random amplified polymorphic DNA (RAPD) fingerprinting. Antibiotic resistance was assessed using the agar dilution method. Virulence factors were identified by polymerase chain reaction and DNA sequencing. Results Among 80 patients, co-infection by two H. pylori strains was detected in 10 patients. Among the 10 pairs of H. pylori strains, differences in antibiotic resistance patterns were detected in 7 pairs (clarithromycin, 1 patient; quinolone, 3 patients; metronidazole, 4 patients) and differences in virulence factors were detected in 5 pairs. The cagA virulence gene was detected in all 10 patients, and 2 patients had H. pylori strains with different EPIYA motifs. Differences in vacA genotypes were detected in 4 patients. Conclusions Co-infection by two H. pylori strains was confirmed by RAPD fingerprinting. Frequently, two H. pylori strains obtained from a single host differed in their virulence factors and antibiotic resistance patterns. Co-infection by multiple H. pylori strains could undermine the success of eradication therapy and should be considered when interpreting the results of antimicrobial susceptibility tests.http://link.springer.com/article/10.1186/s12876-019-1062-5Random amplified polymorphic DNAHelicobacter pyloriVirulence factorAntibiotic resistance |
| spellingShingle | Ji Won Seo Jae Yong Park Tae-Seop Shin Jae Gyu Kim The analysis of virulence factors and antibiotic resistance between Helicobacter pylori strains isolated from gastric antrum and body BMC Gastroenterology Random amplified polymorphic DNA Helicobacter pylori Virulence factor Antibiotic resistance |
| title | The analysis of virulence factors and antibiotic resistance between Helicobacter pylori strains isolated from gastric antrum and body |
| title_full | The analysis of virulence factors and antibiotic resistance between Helicobacter pylori strains isolated from gastric antrum and body |
| title_fullStr | The analysis of virulence factors and antibiotic resistance between Helicobacter pylori strains isolated from gastric antrum and body |
| title_full_unstemmed | The analysis of virulence factors and antibiotic resistance between Helicobacter pylori strains isolated from gastric antrum and body |
| title_short | The analysis of virulence factors and antibiotic resistance between Helicobacter pylori strains isolated from gastric antrum and body |
| title_sort | analysis of virulence factors and antibiotic resistance between helicobacter pylori strains isolated from gastric antrum and body |
| topic | Random amplified polymorphic DNA Helicobacter pylori Virulence factor Antibiotic resistance |
| url | http://link.springer.com/article/10.1186/s12876-019-1062-5 |
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