Tylophora hirsuta L. leaf extract attenuates alloxan-induced diabetes in mice by suppressing oxidative stress and α-amylase
Objective: To evaluate the antidiabetic potential of leaf extracts of Tylophora hirsuta (T. hirsuta). Methods: The methanolic and ethyl acetate extracts of T. hirsuta leaves were analyzed by high pressure liquid chromatography. In vitro antioxidant activity was determined by ferric ion reduction, 1,...
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Format: | Article |
Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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Series: | Asian Pacific Journal of Tropical Biomedicine |
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Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=9;spage=394;epage=404;aulast=Akhtar |
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author | Muhammad Furqan Akhtar Arsalan Shagufta Ammara Saleem Mirza Muhammad Faran Ashraf Baig Ali Sharif Azhar Rasul Mohamed M Abdel-Daim |
author_facet | Muhammad Furqan Akhtar Arsalan Shagufta Ammara Saleem Mirza Muhammad Faran Ashraf Baig Ali Sharif Azhar Rasul Mohamed M Abdel-Daim |
author_sort | Muhammad Furqan Akhtar |
collection | DOAJ |
description | Objective: To evaluate the antidiabetic potential of leaf extracts of Tylophora hirsuta (T. hirsuta).
Methods: The methanolic and ethyl acetate extracts of T. hirsuta leaves were analyzed by high pressure liquid chromatography. In vitro antioxidant activity was determined by ferric ion reduction, 1, 1-diphenyl-2-picrylhydrazyl, and hydrogen peroxide scavenging methods. In vitro alpha amylase (α-amylase) inhibitory activity of the plant extracts was assessed. In vivo antidiabetic potential was determined in alloxan-induced diabetic mice to assess glycated hemoglobin (HbA1c), oral glucose tolerance, serum amylase, lipid profile, fasting blood glucose, and body weight. Histopathological lesions of the pancreas, liver and kidney were observed. Oxidative stress biomarkers such as superoxide dismutase, catalase and peroxidase were also determined.
Results: Quercetin, chlorogenic acid, p-coumaric acid, and m-coumaric acid were found in the plant extracts. The methanolic plant extract exhibited higher in vitro antioxidant activities than the ethyl acetate extract. Moreover, methanolic plant extract exhibited (83.90±1.56)% α-amylase inhibitory activity at 3.2 mg/ mL concentration. Animal study showed that the methanolic extract of T. hirsuta improved the levels of fasting blood glucose, HbA1c, serum α-amylase, lipid profile, liver function biomarkers, and kidney functions of diabetic mice. Moreover, the methanolic extract ameliorated diabetes-related oxidative stress by increasing superoxide dismutase and catalase activities and decreasing peroxidase and malondialdehyde levels. Histopathological examination showed that the plant extract had improved the integrity of pancreatic islets of Langerhans and reduced the pathological lesions in the liver and kidney of diabetic mice.
Conclusions: The methanolic extract of T. hirsuta exhibits pronounced antidiabetic activity in mice through reduction of oxidative stress. The plant extract has several natural antioxidants such as phenolic acids. T. hirsuta extract could serve as a nutraceutical for managing diabetes mellitus. |
first_indexed | 2024-04-11T10:57:24Z |
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issn | 2221-1691 2588-9222 |
language | English |
last_indexed | 2024-04-11T10:57:24Z |
publishDate | 2021-01-01 |
publisher | Wolters Kluwer Medknow Publications |
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series | Asian Pacific Journal of Tropical Biomedicine |
spelling | doaj.art-6575c3a6870748ceb3e3cb68bdb48cb72022-12-22T04:28:43ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222021-01-0111939440410.4103/2221-1691.321128Tylophora hirsuta L. leaf extract attenuates alloxan-induced diabetes in mice by suppressing oxidative stress and α-amylaseMuhammad Furqan AkhtarArsalan ShaguftaAmmara SaleemMirza Muhammad Faran Ashraf BaigAli SharifAzhar RasulMohamed M Abdel-DaimObjective: To evaluate the antidiabetic potential of leaf extracts of Tylophora hirsuta (T. hirsuta). Methods: The methanolic and ethyl acetate extracts of T. hirsuta leaves were analyzed by high pressure liquid chromatography. In vitro antioxidant activity was determined by ferric ion reduction, 1, 1-diphenyl-2-picrylhydrazyl, and hydrogen peroxide scavenging methods. In vitro alpha amylase (α-amylase) inhibitory activity of the plant extracts was assessed. In vivo antidiabetic potential was determined in alloxan-induced diabetic mice to assess glycated hemoglobin (HbA1c), oral glucose tolerance, serum amylase, lipid profile, fasting blood glucose, and body weight. Histopathological lesions of the pancreas, liver and kidney were observed. Oxidative stress biomarkers such as superoxide dismutase, catalase and peroxidase were also determined. Results: Quercetin, chlorogenic acid, p-coumaric acid, and m-coumaric acid were found in the plant extracts. The methanolic plant extract exhibited higher in vitro antioxidant activities than the ethyl acetate extract. Moreover, methanolic plant extract exhibited (83.90±1.56)% α-amylase inhibitory activity at 3.2 mg/ mL concentration. Animal study showed that the methanolic extract of T. hirsuta improved the levels of fasting blood glucose, HbA1c, serum α-amylase, lipid profile, liver function biomarkers, and kidney functions of diabetic mice. Moreover, the methanolic extract ameliorated diabetes-related oxidative stress by increasing superoxide dismutase and catalase activities and decreasing peroxidase and malondialdehyde levels. Histopathological examination showed that the plant extract had improved the integrity of pancreatic islets of Langerhans and reduced the pathological lesions in the liver and kidney of diabetic mice. Conclusions: The methanolic extract of T. hirsuta exhibits pronounced antidiabetic activity in mice through reduction of oxidative stress. The plant extract has several natural antioxidants such as phenolic acids. T. hirsuta extract could serve as a nutraceutical for managing diabetes mellitus.http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=9;spage=394;epage=404;aulast=Akhtartylophora hirsuta; diabetes mellitus; oxidative stress; alpha-amylase; phenolic compounds; pancreas |
spellingShingle | Muhammad Furqan Akhtar Arsalan Shagufta Ammara Saleem Mirza Muhammad Faran Ashraf Baig Ali Sharif Azhar Rasul Mohamed M Abdel-Daim Tylophora hirsuta L. leaf extract attenuates alloxan-induced diabetes in mice by suppressing oxidative stress and α-amylase Asian Pacific Journal of Tropical Biomedicine tylophora hirsuta; diabetes mellitus; oxidative stress; alpha-amylase; phenolic compounds; pancreas |
title | Tylophora hirsuta L. leaf extract attenuates alloxan-induced diabetes in mice by suppressing oxidative stress and α-amylase |
title_full | Tylophora hirsuta L. leaf extract attenuates alloxan-induced diabetes in mice by suppressing oxidative stress and α-amylase |
title_fullStr | Tylophora hirsuta L. leaf extract attenuates alloxan-induced diabetes in mice by suppressing oxidative stress and α-amylase |
title_full_unstemmed | Tylophora hirsuta L. leaf extract attenuates alloxan-induced diabetes in mice by suppressing oxidative stress and α-amylase |
title_short | Tylophora hirsuta L. leaf extract attenuates alloxan-induced diabetes in mice by suppressing oxidative stress and α-amylase |
title_sort | tylophora hirsuta l leaf extract attenuates alloxan induced diabetes in mice by suppressing oxidative stress and α amylase |
topic | tylophora hirsuta; diabetes mellitus; oxidative stress; alpha-amylase; phenolic compounds; pancreas |
url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=9;spage=394;epage=404;aulast=Akhtar |
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