Genome-wide identification of the SPL gene family in Tartary Buckwheat (Fagopyrum tataricum) and expression analysis during fruit development stages
Abstract Background SPL (SQUAMOSA promoter binding protein-like) is a class of plant-specific transcription factors that play important roles in many growth and developmental processes, including shoot and inflorescence branching, embryonic development, signal transduction, leaf initiation, phase tr...
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| Format: | Article |
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BMC
2019-07-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-019-1916-6 |
| _version_ | 1818612825704103936 |
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| author | Moyang Liu Wenjun Sun Zhaotang Ma Li Huang Qi Wu Zizhong Tang Tongliang Bu Chenglei Li Hui Chen |
| author_facet | Moyang Liu Wenjun Sun Zhaotang Ma Li Huang Qi Wu Zizhong Tang Tongliang Bu Chenglei Li Hui Chen |
| author_sort | Moyang Liu |
| collection | DOAJ |
| description | Abstract Background SPL (SQUAMOSA promoter binding protein-like) is a class of plant-specific transcription factors that play important roles in many growth and developmental processes, including shoot and inflorescence branching, embryonic development, signal transduction, leaf initiation, phase transition, and flower and fruit development. The SPL gene family has been identified and characterized in many species but has not been well studied in tartary buckwheat, which is an important edible and medicinal crop. Results In this study, 24 Fagopyrum tataricum SPL (FtSPL) genes were identified and renamed according to the chromosomal distribution of the FtSPL genes. According to the amino acid sequence of the SBP domain and gene structure, the SPL genes were divided into eight groups (group I to group VII) by phylogenetic tree analysis. A total of 10 motifs were detected in the tartary buckwheat SPL genes. The expression patterns of 23 SPL genes in different tissues and fruits at different developmental stages (green fruit stage, discoloration stage and initial maturity stage) were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Conclusions The tartary buckwheat genome contained 24 SPL genes, and most of the genes were expressed in different tissues. qRT-PCR showed that FtSPLs played important roles in the growth and development of tartary buckwheat, and genes that might regulate flower and fruit development were preliminarily identified. This work provides a comprehensive understanding of the SBP-box gene family in tartary buckwheat and lays a significant foundation for further studies on the functional characteristics of FtSPL genes and improvement of tartary buckwheat crops. |
| first_indexed | 2024-12-16T15:52:23Z |
| format | Article |
| id | doaj.art-65763ee1bbbe402889d337ca2e8bb999 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-16T15:52:23Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-65763ee1bbbe402889d337ca2e8bb9992022-12-21T22:25:40ZengBMCBMC Plant Biology1471-22292019-07-0119111410.1186/s12870-019-1916-6Genome-wide identification of the SPL gene family in Tartary Buckwheat (Fagopyrum tataricum) and expression analysis during fruit development stagesMoyang Liu0Wenjun Sun1Zhaotang Ma2Li Huang3Qi Wu4Zizhong Tang5Tongliang Bu6Chenglei Li7Hui Chen8College of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityCollege of Life Science, Sichuan Agricultural UniversityAbstract Background SPL (SQUAMOSA promoter binding protein-like) is a class of plant-specific transcription factors that play important roles in many growth and developmental processes, including shoot and inflorescence branching, embryonic development, signal transduction, leaf initiation, phase transition, and flower and fruit development. The SPL gene family has been identified and characterized in many species but has not been well studied in tartary buckwheat, which is an important edible and medicinal crop. Results In this study, 24 Fagopyrum tataricum SPL (FtSPL) genes were identified and renamed according to the chromosomal distribution of the FtSPL genes. According to the amino acid sequence of the SBP domain and gene structure, the SPL genes were divided into eight groups (group I to group VII) by phylogenetic tree analysis. A total of 10 motifs were detected in the tartary buckwheat SPL genes. The expression patterns of 23 SPL genes in different tissues and fruits at different developmental stages (green fruit stage, discoloration stage and initial maturity stage) were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Conclusions The tartary buckwheat genome contained 24 SPL genes, and most of the genes were expressed in different tissues. qRT-PCR showed that FtSPLs played important roles in the growth and development of tartary buckwheat, and genes that might regulate flower and fruit development were preliminarily identified. This work provides a comprehensive understanding of the SBP-box gene family in tartary buckwheat and lays a significant foundation for further studies on the functional characteristics of FtSPL genes and improvement of tartary buckwheat crops.http://link.springer.com/article/10.1186/s12870-019-1916-6Tartary buckwheatFtSPLGenomeFruit developmentExpression patterns |
| spellingShingle | Moyang Liu Wenjun Sun Zhaotang Ma Li Huang Qi Wu Zizhong Tang Tongliang Bu Chenglei Li Hui Chen Genome-wide identification of the SPL gene family in Tartary Buckwheat (Fagopyrum tataricum) and expression analysis during fruit development stages BMC Plant Biology Tartary buckwheat FtSPL Genome Fruit development Expression patterns |
| title | Genome-wide identification of the SPL gene family in Tartary Buckwheat (Fagopyrum tataricum) and expression analysis during fruit development stages |
| title_full | Genome-wide identification of the SPL gene family in Tartary Buckwheat (Fagopyrum tataricum) and expression analysis during fruit development stages |
| title_fullStr | Genome-wide identification of the SPL gene family in Tartary Buckwheat (Fagopyrum tataricum) and expression analysis during fruit development stages |
| title_full_unstemmed | Genome-wide identification of the SPL gene family in Tartary Buckwheat (Fagopyrum tataricum) and expression analysis during fruit development stages |
| title_short | Genome-wide identification of the SPL gene family in Tartary Buckwheat (Fagopyrum tataricum) and expression analysis during fruit development stages |
| title_sort | genome wide identification of the spl gene family in tartary buckwheat fagopyrum tataricum and expression analysis during fruit development stages |
| topic | Tartary buckwheat FtSPL Genome Fruit development Expression patterns |
| url | http://link.springer.com/article/10.1186/s12870-019-1916-6 |
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