Photo-/thermo-responsive bioink for improved printability in extrusion-based bioprinting
Extrusion-based bioprinting has demonstrated significant potential for manufacturing constructs, particularly for 3D cell culture. However, there is a greatly limited number of bioink candidates exploited with extrusion-based bioprinting, as they meet the opposing requirements for printability with...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006424000322 |
| _version_ | 1797258660264542208 |
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| author | Seo Hyung Moon Tae Yoon Park Hyung Joon Cha Yun Jung Yang |
| author_facet | Seo Hyung Moon Tae Yoon Park Hyung Joon Cha Yun Jung Yang |
| author_sort | Seo Hyung Moon |
| collection | DOAJ |
| description | Extrusion-based bioprinting has demonstrated significant potential for manufacturing constructs, particularly for 3D cell culture. However, there is a greatly limited number of bioink candidates exploited with extrusion-based bioprinting, as they meet the opposing requirements for printability with indispensable rheological features and for biochemical functionality with desirable microenvironment. In this study, a blend of silk fibroin (SF) and iota-carrageenan (CG) was chosen as a cell-friendly printable material. The SF/CG ink exhibited suitable viscosity and shear-thinning properties, coupled with the rapid sol-gel transition of CG. By employing photo-crosslinking of SF, the printability with Pr value close to 1 and structural integrity of the 3D constructs were significantly improved within a matter of seconds. The printed constructs demonstrated a Young's modulus of approximately 250 kPa, making them suitable for keratinocyte and myoblast cell culture. Furthermore, the high cell adhesiveness and viability (maximum >98%) of the loaded cells underscored the considerable potential of this 3D culture scaffold applied for skin and muscle tissues, which can be easily manipulated using an extrusion-based bioprinter. |
| first_indexed | 2024-03-08T09:27:50Z |
| format | Article |
| id | doaj.art-65785c4c7e5843a5b7e2b81f851d2a4a |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-24T22:57:04Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-65785c4c7e5843a5b7e2b81f851d2a4a2024-03-18T04:34:19ZengElsevierMaterials Today Bio2590-00642024-04-0125100973Photo-/thermo-responsive bioink for improved printability in extrusion-based bioprintingSeo Hyung Moon0Tae Yoon Park1Hyung Joon Cha2Yun Jung Yang3Department of Biological Sciences and Bioengineering, Inha University, Incheon, 22212, Republic of KoreaDepartment of Chemical Engineering, Pohang University of Science and Technology, Pohang, 37673, Republic of KoreaDepartment of Chemical Engineering, Pohang University of Science and Technology, Pohang, 37673, Republic of Korea; Medical Science and Engineering, School of Convergence Science and Technology, Pohang University of Science and Technology, Pohang, 37673, Republic of KoreaDepartment of Biological Sciences and Bioengineering, Inha University, Incheon, 22212, Republic of Korea; Inha University Hospital, Incheon, 22332, Republic of Korea; Corresponding author. Department of Biological Sciences and Bioengineering, Inha University, Incheon, 22212, Republic of Korea.Extrusion-based bioprinting has demonstrated significant potential for manufacturing constructs, particularly for 3D cell culture. However, there is a greatly limited number of bioink candidates exploited with extrusion-based bioprinting, as they meet the opposing requirements for printability with indispensable rheological features and for biochemical functionality with desirable microenvironment. In this study, a blend of silk fibroin (SF) and iota-carrageenan (CG) was chosen as a cell-friendly printable material. The SF/CG ink exhibited suitable viscosity and shear-thinning properties, coupled with the rapid sol-gel transition of CG. By employing photo-crosslinking of SF, the printability with Pr value close to 1 and structural integrity of the 3D constructs were significantly improved within a matter of seconds. The printed constructs demonstrated a Young's modulus of approximately 250 kPa, making them suitable for keratinocyte and myoblast cell culture. Furthermore, the high cell adhesiveness and viability (maximum >98%) of the loaded cells underscored the considerable potential of this 3D culture scaffold applied for skin and muscle tissues, which can be easily manipulated using an extrusion-based bioprinter.http://www.sciencedirect.com/science/article/pii/S2590006424000322Silk fibroinIota-carrageenanBioinkExtrusion-based bioprintingDual-crosslinking |
| spellingShingle | Seo Hyung Moon Tae Yoon Park Hyung Joon Cha Yun Jung Yang Photo-/thermo-responsive bioink for improved printability in extrusion-based bioprinting Materials Today Bio Silk fibroin Iota-carrageenan Bioink Extrusion-based bioprinting Dual-crosslinking |
| title | Photo-/thermo-responsive bioink for improved printability in extrusion-based bioprinting |
| title_full | Photo-/thermo-responsive bioink for improved printability in extrusion-based bioprinting |
| title_fullStr | Photo-/thermo-responsive bioink for improved printability in extrusion-based bioprinting |
| title_full_unstemmed | Photo-/thermo-responsive bioink for improved printability in extrusion-based bioprinting |
| title_short | Photo-/thermo-responsive bioink for improved printability in extrusion-based bioprinting |
| title_sort | photo thermo responsive bioink for improved printability in extrusion based bioprinting |
| topic | Silk fibroin Iota-carrageenan Bioink Extrusion-based bioprinting Dual-crosslinking |
| url | http://www.sciencedirect.com/science/article/pii/S2590006424000322 |
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