| Summary: | During a survey of aquatic fungi from Anzali Lagoon in Iran, several fungal specimens were isolated from freshwater habitats. Morphological evidence and comparing sequencing based on rDNA (ITS and LSU) and protein-coding genes (<i>TEF1</i> and <i>TUB2</i>) showed that some isolates belong to undescribed fungal species. These isolates belong to <i>Arthrobotrys</i> and <i>Sarocladium</i>, two ascomycetes genera. <i>Arthrobotrys</i> <i>hyrcanus</i>, sp. nov., differs from closely related species such as <i>A. dianchiensis</i> by its larger conidia and septation of primary conidia. <i>Sarocladium</i> <i>pseudokiliense</i>, sp. nov., was similar to <i>S. kiliense</i>, but distinguished by its conidial shape and the absence of adelophialides and chlamydospores. Morphological descriptions, illustrations and multilocus phylogenetic analysis for both new species are provided.
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