Histochemical localization of sialic acids and antimicrobial substances in eccrine glands of porcine snout skin
The distribution of sialic acids and antimicrobial products (lysozyme, IgA, lactoferrin, β-defensin 2) as well as Rab3D in the eccrine glands of porcine snout skin was studied by sialoglycoconjugate histochemistry and immunohistochemistry. The secretory epithelium consisted of two types of secretory...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2012-02-01
|
| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://www.ejh.it/index.php/ejh/article/view/1924 |
| _version_ | 1828780976332865536 |
|---|---|
| author | K. Fukui T. Yasui H. Gomi H. Sugiya O. Fujimori W. Meyer A. Tsukise |
| author_facet | K. Fukui T. Yasui H. Gomi H. Sugiya O. Fujimori W. Meyer A. Tsukise |
| author_sort | K. Fukui |
| collection | DOAJ |
| description | The distribution of sialic acids and antimicrobial products (lysozyme, IgA, lactoferrin, β-defensin 2) as well as Rab3D in the eccrine glands of porcine snout skin was studied by sialoglycoconjugate histochemistry and immunohistochemistry. The secretory epithelium consisted of two types of secretory cells: dark and clear cells. The dark cells exhibited considerable amounts of sialoglycoconjugates, which included O-acetylated sialic acids, whereas sialic acids in the sequence Siaα2-3Gal1-4GlcNAc were confined to some of the dark cells. All antimicrobial substances and Rab3D were demonstrated to be also mainly present in some of the dark cells. Additionally, in the cytological and cytochemical features, the different characteristics were observed among the dark cells. The results obtained are discussed with regard to the functional significance of the eccrine glands. The secretory products elaborated by this gland type may function as protective agents in order to preserve the skin integrity of the snout region, considering that sialic acids and antimicrobial substances are important in general defense mechanisms. |
| first_indexed | 2024-12-11T17:26:18Z |
| format | Article |
| id | doaj.art-657b72bb94324d83af992d19a97c3164 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-11T17:26:18Z |
| publishDate | 2012-02-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-657b72bb94324d83af992d19a97c31642022-12-22T00:56:58ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062012-02-01561e6e610.4081/ejh.2012.e61249Histochemical localization of sialic acids and antimicrobial substances in eccrine glands of porcine snout skinK. Fukui0T. Yasui1H. Gomi2H. Sugiya3O. Fujimori4W. Meyer5A. Tsukise6Department of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, 1866, Fujisawa; KanagawaDepartment of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, KanagawaDepartment of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, KanagawaLaboratory of Veterinary Biochemistry, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, KanagawaLaboratory of Anatomy and Histochemistry, Faculty of Rehabilitation Science, Nagoya Gakuin University, 1350 Kamishinanocho, Seto AichiInstitute for Anatomy, University of Veterinary Medicine Hannover FoundationDepartment of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, KanagawaThe distribution of sialic acids and antimicrobial products (lysozyme, IgA, lactoferrin, β-defensin 2) as well as Rab3D in the eccrine glands of porcine snout skin was studied by sialoglycoconjugate histochemistry and immunohistochemistry. The secretory epithelium consisted of two types of secretory cells: dark and clear cells. The dark cells exhibited considerable amounts of sialoglycoconjugates, which included O-acetylated sialic acids, whereas sialic acids in the sequence Siaα2-3Gal1-4GlcNAc were confined to some of the dark cells. All antimicrobial substances and Rab3D were demonstrated to be also mainly present in some of the dark cells. Additionally, in the cytological and cytochemical features, the different characteristics were observed among the dark cells. The results obtained are discussed with regard to the functional significance of the eccrine glands. The secretory products elaborated by this gland type may function as protective agents in order to preserve the skin integrity of the snout region, considering that sialic acids and antimicrobial substances are important in general defense mechanisms.http://www.ejh.it/index.php/ejh/article/view/1924sialic acids, antimicrobial substances, eccrine glands, porcine snout skin, histochemistry |
| spellingShingle | K. Fukui T. Yasui H. Gomi H. Sugiya O. Fujimori W. Meyer A. Tsukise Histochemical localization of sialic acids and antimicrobial substances in eccrine glands of porcine snout skin European Journal of Histochemistry sialic acids, antimicrobial substances, eccrine glands, porcine snout skin, histochemistry |
| title | Histochemical localization of sialic acids and antimicrobial substances in eccrine glands of porcine snout skin |
| title_full | Histochemical localization of sialic acids and antimicrobial substances in eccrine glands of porcine snout skin |
| title_fullStr | Histochemical localization of sialic acids and antimicrobial substances in eccrine glands of porcine snout skin |
| title_full_unstemmed | Histochemical localization of sialic acids and antimicrobial substances in eccrine glands of porcine snout skin |
| title_short | Histochemical localization of sialic acids and antimicrobial substances in eccrine glands of porcine snout skin |
| title_sort | histochemical localization of sialic acids and antimicrobial substances in eccrine glands of porcine snout skin |
| topic | sialic acids, antimicrobial substances, eccrine glands, porcine snout skin, histochemistry |
| url | http://www.ejh.it/index.php/ejh/article/view/1924 |
| work_keys_str_mv | AT kfukui histochemicallocalizationofsialicacidsandantimicrobialsubstancesineccrineglandsofporcinesnoutskin AT tyasui histochemicallocalizationofsialicacidsandantimicrobialsubstancesineccrineglandsofporcinesnoutskin AT hgomi histochemicallocalizationofsialicacidsandantimicrobialsubstancesineccrineglandsofporcinesnoutskin AT hsugiya histochemicallocalizationofsialicacidsandantimicrobialsubstancesineccrineglandsofporcinesnoutskin AT ofujimori histochemicallocalizationofsialicacidsandantimicrobialsubstancesineccrineglandsofporcinesnoutskin AT wmeyer histochemicallocalizationofsialicacidsandantimicrobialsubstancesineccrineglandsofporcinesnoutskin AT atsukise histochemicallocalizationofsialicacidsandantimicrobialsubstancesineccrineglandsofporcinesnoutskin |