Single-dose erythropoietin does not reduce apoptosis in extracorporeal preserved inguinal fat flaps of the rat
Adipose tissue apoptosis is of interest in many fields of medicine. Volume loss due to apoptosis for example remains a major sequela of lipofilling procedures. The aim of the study was to reduce apoptosis of fat cells by administering erythropoietin (EPO), known as an anti-apoptotic acting hormone...
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| Format: | Article |
| Language: | deu |
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German Medical Science GMS Publishing House
2016-08-01
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| Series: | GMS German Plastic, Reconstructive and Aesthetic Surgery – Burn and Hand Surgery |
| Online Access: | http://www.egms.de/static/en/journals/gpras/2016-6/gpras000040.shtml |
| _version_ | 1818334484072759296 |
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| author | Herold, Christian Rennekampff, Hans O. Ohm, Lea Sorg, Heiko Vaske, Bernhard Vogt, Peter M. |
| author_facet | Herold, Christian Rennekampff, Hans O. Ohm, Lea Sorg, Heiko Vaske, Bernhard Vogt, Peter M. |
| author_sort | Herold, Christian |
| collection | DOAJ |
| description | Adipose tissue apoptosis is of interest in many fields of medicine. Volume loss due to apoptosis for example remains a major sequela of lipofilling procedures. The aim of the study was to reduce apoptosis of fat cells by administering erythropoietin (EPO), known as an anti-apoptotic acting hormone in vitro, to ex vivo preserved fat flaps.A single dose of 1,000 IE EPO was injected into the inguinal adipofascial flap of rats (n=5) directly after explantation via the femoral artery. The flap was preserved in a bioreactor by continuous perfusion with Hannover solution for ten days. Immunohistochemistry for caspase 3 and EPO receptor was performed, at the following time points: 0, 8, 24, 48 and 240 hours.Compared to adipofascial flaps without pretreatment with EPO, flaps which were treated with EPO did not show a significant reduced level of cleaved caspase 3 activity. In both control and EPO treated tissue, a similar constant increase in cleaved caspase 3 activity was observed. Analysis of EPO receptor revealed that there was no immunoreactivity of EPO receptor in the inguinal fat flaps of the rat.In contrast to in vivo studies we could not demonstrate a beneficial effect of single dose EPO on cell survival in fat flap ex vivo in rats. This is probably due to the lack of the EPO receptor on adipose cells. |
| first_indexed | 2024-12-13T14:08:16Z |
| format | Article |
| id | doaj.art-657baa6651264b469e62e1119c8cd717 |
| institution | Directory Open Access Journal |
| issn | 2193-7052 |
| language | deu |
| last_indexed | 2024-12-13T14:08:16Z |
| publishDate | 2016-08-01 |
| publisher | German Medical Science GMS Publishing House |
| record_format | Article |
| series | GMS German Plastic, Reconstructive and Aesthetic Surgery – Burn and Hand Surgery |
| spelling | doaj.art-657baa6651264b469e62e1119c8cd7172022-12-21T23:42:33ZdeuGerman Medical Science GMS Publishing HouseGMS German Plastic, Reconstructive and Aesthetic Surgery – Burn and Hand Surgery2193-70522016-08-016Doc0510.3205/gpras000040Single-dose erythropoietin does not reduce apoptosis in extracorporeal preserved inguinal fat flaps of the ratHerold, Christian0Rennekampff, Hans O.1Ohm, Lea2Sorg, Heiko3Vaske, Bernhard4Vogt, Peter M.5Klinik für Plastische und Ästhetische Chirurgie / Handchirurgie, Sana Klinikum Hameln-Pyrmont, Hameln, GermanyPlastische & Ästhetische Chirurgie / Verbrennungschirurgie, Klinik für Orthopädie, Unfall-, Hand- und Wiederherstellungschirurgie, Klinikum Leverkusen gGmbH, Leverkusen, GermanyChirurgische Klinik I – Klinik für Orthopädie und Unfallchirurgie, KRH Klinikum Großburgwedel, GermanyKlinik für Plastische, Rekonstruktive und Ästhetische Chirurgie / Handchirurgie, Alfried Krupp Krankenhaus, Essen, GermanyInstitut für Biometrie, Medizinische Hochschule Hannover, GermanyKlinik für Plastische, Ästhetische, Hand- und Wiederherstellungschirurgie, Medizinische Hochschule Hannover, GermanyAdipose tissue apoptosis is of interest in many fields of medicine. Volume loss due to apoptosis for example remains a major sequela of lipofilling procedures. The aim of the study was to reduce apoptosis of fat cells by administering erythropoietin (EPO), known as an anti-apoptotic acting hormone in vitro, to ex vivo preserved fat flaps.A single dose of 1,000 IE EPO was injected into the inguinal adipofascial flap of rats (n=5) directly after explantation via the femoral artery. The flap was preserved in a bioreactor by continuous perfusion with Hannover solution for ten days. Immunohistochemistry for caspase 3 and EPO receptor was performed, at the following time points: 0, 8, 24, 48 and 240 hours.Compared to adipofascial flaps without pretreatment with EPO, flaps which were treated with EPO did not show a significant reduced level of cleaved caspase 3 activity. In both control and EPO treated tissue, a similar constant increase in cleaved caspase 3 activity was observed. Analysis of EPO receptor revealed that there was no immunoreactivity of EPO receptor in the inguinal fat flaps of the rat.In contrast to in vivo studies we could not demonstrate a beneficial effect of single dose EPO on cell survival in fat flap ex vivo in rats. This is probably due to the lack of the EPO receptor on adipose cells.http://www.egms.de/static/en/journals/gpras/2016-6/gpras000040.shtml |
| spellingShingle | Herold, Christian Rennekampff, Hans O. Ohm, Lea Sorg, Heiko Vaske, Bernhard Vogt, Peter M. Single-dose erythropoietin does not reduce apoptosis in extracorporeal preserved inguinal fat flaps of the rat GMS German Plastic, Reconstructive and Aesthetic Surgery – Burn and Hand Surgery |
| title | Single-dose erythropoietin does not reduce apoptosis in extracorporeal preserved inguinal fat flaps of the rat |
| title_full | Single-dose erythropoietin does not reduce apoptosis in extracorporeal preserved inguinal fat flaps of the rat |
| title_fullStr | Single-dose erythropoietin does not reduce apoptosis in extracorporeal preserved inguinal fat flaps of the rat |
| title_full_unstemmed | Single-dose erythropoietin does not reduce apoptosis in extracorporeal preserved inguinal fat flaps of the rat |
| title_short | Single-dose erythropoietin does not reduce apoptosis in extracorporeal preserved inguinal fat flaps of the rat |
| title_sort | single dose erythropoietin does not reduce apoptosis in extracorporeal preserved inguinal fat flaps of the rat |
| url | http://www.egms.de/static/en/journals/gpras/2016-6/gpras000040.shtml |
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