Genetically Modified Sugarcane Intercropping Soybean Impact on Rhizosphere Bacterial Communities and Co-occurrence Patterns
Strategies involving genes in the dehydration-responsive element binding (DREB) family, which participates in drought stress regulation, and intercropping with legumes are becoming prominent options in promoting sustainable sugarcane cultivation. An increasing number of studies focusing on root inte...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.742341/full |
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| author | Beilei Wei Beilei Wei Beilei Wei Jinlian Zhang Jinlian Zhang Rushuang Wen Tingsu Chen Ningshao Xia Yue Liu Yue Liu Ziting Wang Ziting Wang |
| author_facet | Beilei Wei Beilei Wei Beilei Wei Jinlian Zhang Jinlian Zhang Rushuang Wen Tingsu Chen Ningshao Xia Yue Liu Yue Liu Ziting Wang Ziting Wang |
| author_sort | Beilei Wei |
| collection | DOAJ |
| description | Strategies involving genes in the dehydration-responsive element binding (DREB) family, which participates in drought stress regulation, and intercropping with legumes are becoming prominent options in promoting sustainable sugarcane cultivation. An increasing number of studies focusing on root interactions in intercropping systems, particularly involving transgenic crops, are being conducted to better understand and thus, harness beneficial soil microbes to enhance plant growth. We designed experiments to investigate the characteristics of two intercropping patterns, soybean with wild-type (WT) sugarcane and soybean with genetically modified (GM) Ea-DREB2B-overexpressing sugarcane, to assess the response of the rhizosphere microbiota to the different cropping patterns. Bacterial diversity in the rhizosphere microbial community differed between the two intercropping pattens. In addition, the biomass of GM sugarcane that intercropped with soybean was significantly improved compared with WT sugarcane, and the aboveground biomass and root biomass of GM soybean intercropping sugarcane increased by 49.15 and 46.03% compared with monoculture. Furthermore, a beneficial rhizosphere environment for the growth of Actinobacteria was established in the systems intercropped with GM sugarcane. Improving the production mode of crops by genetic modification is a key strategy to improving crop yields and provides new opportunities to further investigate the effects of intercropping on plant roots and soil microbiota. Thus, this study provides a basis for selecting suitable sugarcane–soybean intercropping patterns and a theoretical foundation for a sustainable sugarcane production. |
| first_indexed | 2024-12-23T23:08:43Z |
| format | Article |
| id | doaj.art-657d5d86735b4684ba01abe1dc4ed81d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-23T23:08:43Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-657d5d86735b4684ba01abe1dc4ed81d2022-12-21T17:26:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-12-011210.3389/fmicb.2021.742341742341Genetically Modified Sugarcane Intercropping Soybean Impact on Rhizosphere Bacterial Communities and Co-occurrence PatternsBeilei Wei0Beilei Wei1Beilei Wei2Jinlian Zhang3Jinlian Zhang4Rushuang Wen5Tingsu Chen6Ningshao Xia7Yue Liu8Yue Liu9Ziting Wang10Ziting Wang11State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, ChinaCollege of Agronomy, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Sugarcane Biology, Nanning, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, ChinaMicrobiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, ChinaCollege of Agronomy, Guangxi University, Nanning, ChinaMicrobiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, ChinaCollege of Agronomy, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Sugarcane Biology, Nanning, ChinaCollege of Agronomy, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Sugarcane Biology, Nanning, ChinaStrategies involving genes in the dehydration-responsive element binding (DREB) family, which participates in drought stress regulation, and intercropping with legumes are becoming prominent options in promoting sustainable sugarcane cultivation. An increasing number of studies focusing on root interactions in intercropping systems, particularly involving transgenic crops, are being conducted to better understand and thus, harness beneficial soil microbes to enhance plant growth. We designed experiments to investigate the characteristics of two intercropping patterns, soybean with wild-type (WT) sugarcane and soybean with genetically modified (GM) Ea-DREB2B-overexpressing sugarcane, to assess the response of the rhizosphere microbiota to the different cropping patterns. Bacterial diversity in the rhizosphere microbial community differed between the two intercropping pattens. In addition, the biomass of GM sugarcane that intercropped with soybean was significantly improved compared with WT sugarcane, and the aboveground biomass and root biomass of GM soybean intercropping sugarcane increased by 49.15 and 46.03% compared with monoculture. Furthermore, a beneficial rhizosphere environment for the growth of Actinobacteria was established in the systems intercropped with GM sugarcane. Improving the production mode of crops by genetic modification is a key strategy to improving crop yields and provides new opportunities to further investigate the effects of intercropping on plant roots and soil microbiota. Thus, this study provides a basis for selecting suitable sugarcane–soybean intercropping patterns and a theoretical foundation for a sustainable sugarcane production.https://www.frontiersin.org/articles/10.3389/fmicb.2021.742341/fullintercroppingtransgenic cropsrhizosphere microbial environmentinteractionsugarcane |
| spellingShingle | Beilei Wei Beilei Wei Beilei Wei Jinlian Zhang Jinlian Zhang Rushuang Wen Tingsu Chen Ningshao Xia Yue Liu Yue Liu Ziting Wang Ziting Wang Genetically Modified Sugarcane Intercropping Soybean Impact on Rhizosphere Bacterial Communities and Co-occurrence Patterns Frontiers in Microbiology intercropping transgenic crops rhizosphere microbial environment interaction sugarcane |
| title | Genetically Modified Sugarcane Intercropping Soybean Impact on Rhizosphere Bacterial Communities and Co-occurrence Patterns |
| title_full | Genetically Modified Sugarcane Intercropping Soybean Impact on Rhizosphere Bacterial Communities and Co-occurrence Patterns |
| title_fullStr | Genetically Modified Sugarcane Intercropping Soybean Impact on Rhizosphere Bacterial Communities and Co-occurrence Patterns |
| title_full_unstemmed | Genetically Modified Sugarcane Intercropping Soybean Impact on Rhizosphere Bacterial Communities and Co-occurrence Patterns |
| title_short | Genetically Modified Sugarcane Intercropping Soybean Impact on Rhizosphere Bacterial Communities and Co-occurrence Patterns |
| title_sort | genetically modified sugarcane intercropping soybean impact on rhizosphere bacterial communities and co occurrence patterns |
| topic | intercropping transgenic crops rhizosphere microbial environment interaction sugarcane |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.742341/full |
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