Upregulation of BCAM and its sense lncRNA BAN are associated with gastric cancer metastasis and poor prognosis
Patients with metastatic gastric cancer (GC) have a poor prognosis; however, the molecular mechanism of GC metastasis remains unclear. Here, we employed bioinformatics to systematically screen the metastasis‐associated genes and found that the levels of basal cell adhesion molecule (BCAM) were signi...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-04-01
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| Series: | Molecular Oncology |
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| Online Access: | https://doi.org/10.1002/1878-0261.12638 |
| _version_ | 1818957752220778496 |
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| author | Juan Jin Shanshan Xie Qiang Sun Zhenxia Huang Kanghua Chen Dongyang Guo Xianping Rao Yujie Deng Yiman Liu Shuang Li Wenyu Cui Valentina Chanu Maibam Junni Wang Wei Zhuo Tianhua Zhou |
| author_facet | Juan Jin Shanshan Xie Qiang Sun Zhenxia Huang Kanghua Chen Dongyang Guo Xianping Rao Yujie Deng Yiman Liu Shuang Li Wenyu Cui Valentina Chanu Maibam Junni Wang Wei Zhuo Tianhua Zhou |
| author_sort | Juan Jin |
| collection | DOAJ |
| description | Patients with metastatic gastric cancer (GC) have a poor prognosis; however, the molecular mechanism of GC metastasis remains unclear. Here, we employed bioinformatics to systematically screen the metastasis‐associated genes and found that the levels of basal cell adhesion molecule (BCAM) were significantly increased in GC tissues from patients with metastasis, as compared to those without metastasis. The upregulation of BCAM was also significantly associated with a shorter survival time. Depletion of BCAM inhibited GC cell migration and invasion. Knockout (KO) of BCAM by the CRISPR/Cas9 system reduced the invasion and metastasis of GC cells. To explore the mechanism of BCAM upregulation, we identified a previously uncharacterized BCAM sense lncRNA that spanned from exon 6 to intron 6 of BCAM, and named it as BCAM‐associated long noncoding RNA (BAN). Knockdown of BAN inhibited BCAM expression at both mRNA and protein levels. Knockdown of BAN suppressed GC cell migration and invasion, which was effectively rescued by ectopic expression of BCAM. Further clinical data showed that BAN upregulation was associated with GC metastasis and poor prognosis. Importantly, BAN expression was also significantly associated with that of BCAM in GC tissues. Taken together, these results indicate that increased expression of BCAM and its sense lncRNA BAN promote GC cell invasion and metastasis, and are associated with poor prognosis of GC patients. |
| first_indexed | 2024-12-20T11:14:50Z |
| format | Article |
| id | doaj.art-657fab7e49fa4e0ca1707759a72f9900 |
| institution | Directory Open Access Journal |
| issn | 1574-7891 1878-0261 |
| language | English |
| last_indexed | 2024-12-20T11:14:50Z |
| publishDate | 2020-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Molecular Oncology |
| spelling | doaj.art-657fab7e49fa4e0ca1707759a72f99002022-12-21T19:42:39ZengWileyMolecular Oncology1574-78911878-02612020-04-0114482984510.1002/1878-0261.12638Upregulation of BCAM and its sense lncRNA BAN are associated with gastric cancer metastasis and poor prognosisJuan Jin0Shanshan Xie1Qiang Sun2Zhenxia Huang3Kanghua Chen4Dongyang Guo5Xianping Rao6Yujie Deng7Yiman Liu8Shuang Li9Wenyu Cui10Valentina Chanu Maibam11Junni Wang12Wei Zhuo13Tianhua Zhou14Department of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou ChinaPatients with metastatic gastric cancer (GC) have a poor prognosis; however, the molecular mechanism of GC metastasis remains unclear. Here, we employed bioinformatics to systematically screen the metastasis‐associated genes and found that the levels of basal cell adhesion molecule (BCAM) were significantly increased in GC tissues from patients with metastasis, as compared to those without metastasis. The upregulation of BCAM was also significantly associated with a shorter survival time. Depletion of BCAM inhibited GC cell migration and invasion. Knockout (KO) of BCAM by the CRISPR/Cas9 system reduced the invasion and metastasis of GC cells. To explore the mechanism of BCAM upregulation, we identified a previously uncharacterized BCAM sense lncRNA that spanned from exon 6 to intron 6 of BCAM, and named it as BCAM‐associated long noncoding RNA (BAN). Knockdown of BAN inhibited BCAM expression at both mRNA and protein levels. Knockdown of BAN suppressed GC cell migration and invasion, which was effectively rescued by ectopic expression of BCAM. Further clinical data showed that BAN upregulation was associated with GC metastasis and poor prognosis. Importantly, BAN expression was also significantly associated with that of BCAM in GC tissues. Taken together, these results indicate that increased expression of BCAM and its sense lncRNA BAN promote GC cell invasion and metastasis, and are associated with poor prognosis of GC patients.https://doi.org/10.1002/1878-0261.12638BCAMgastric cancerlncRNAmetastasisprognosis |
| spellingShingle | Juan Jin Shanshan Xie Qiang Sun Zhenxia Huang Kanghua Chen Dongyang Guo Xianping Rao Yujie Deng Yiman Liu Shuang Li Wenyu Cui Valentina Chanu Maibam Junni Wang Wei Zhuo Tianhua Zhou Upregulation of BCAM and its sense lncRNA BAN are associated with gastric cancer metastasis and poor prognosis Molecular Oncology BCAM gastric cancer lncRNA metastasis prognosis |
| title | Upregulation of BCAM and its sense lncRNA BAN are associated with gastric cancer metastasis and poor prognosis |
| title_full | Upregulation of BCAM and its sense lncRNA BAN are associated with gastric cancer metastasis and poor prognosis |
| title_fullStr | Upregulation of BCAM and its sense lncRNA BAN are associated with gastric cancer metastasis and poor prognosis |
| title_full_unstemmed | Upregulation of BCAM and its sense lncRNA BAN are associated with gastric cancer metastasis and poor prognosis |
| title_short | Upregulation of BCAM and its sense lncRNA BAN are associated with gastric cancer metastasis and poor prognosis |
| title_sort | upregulation of bcam and its sense lncrna ban are associated with gastric cancer metastasis and poor prognosis |
| topic | BCAM gastric cancer lncRNA metastasis prognosis |
| url | https://doi.org/10.1002/1878-0261.12638 |
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