A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitro
The gut microbiota is engaged in multiple interactions affecting host health. Bacteriocins showed the ability of impeding the growth of intestinal pathogenic bacteria and modulating gut microbiota in animals. Few studies have also discovered their regulation on human intestinal flora using an in vit...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.877948/full |
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| author | Jiaqian Pu Shuting Hang Manman Liu Ziqi Chen Jiayi Xiong Yongquan Li Hongchen Wu Xiaodan Zhao Shuxun Liu Qing Gu Ping Li |
| author_facet | Jiaqian Pu Shuting Hang Manman Liu Ziqi Chen Jiayi Xiong Yongquan Li Hongchen Wu Xiaodan Zhao Shuxun Liu Qing Gu Ping Li |
| author_sort | Jiaqian Pu |
| collection | DOAJ |
| description | The gut microbiota is engaged in multiple interactions affecting host health. Bacteriocins showed the ability of impeding the growth of intestinal pathogenic bacteria and modulating gut microbiota in animals. Few studies have also discovered their regulation on human intestinal flora using an in vitro simulated system. However, little is known about their effect on gut microbiota of different enterotypes of human. This work evaluated the modification of the gut microbiota of two enterotypes (ET B and ET P) by the class IIb bacteriocin plantaricin NC8 (PLNC8) by using an in vitro fermentation model of the intestine. Gas chromatography results revealed that PLNC8 had no influence on the gut microbiota’s production of short-chain fatty acids in the subjects’ samples. PLNC8 lowered the Shannon index of ET B’ gut microbiota and the Simpson index of ET P’ gut microbiota, according to 16S rDNA sequencing. In ET B, PLNC8 enhanced the abundance of Bacteroides, Bifidobacterium, Megamonas, Escherichia-Shigella, Parabacteroides, and Lactobacillus while decreasing the abundance of Streptococcus. Prevotella_9, Bifidobacterium, Escherichia-Shigella, Mitsuokella, and Collinsella were found more abundant in ET P. The current study adds to our understanding of the impact of PLNC8 on the human gut microbiota and lays the groundwork for future research into PLNC8’s effects on human intestinal disease. |
| first_indexed | 2024-04-13T15:43:03Z |
| format | Article |
| id | doaj.art-6584fe6af2c645f9b16be683cca6f82c |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-13T15:43:03Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-6584fe6af2c645f9b16be683cca6f82c2022-12-22T02:41:04ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-06-01910.3389/fnut.2022.877948877948A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitroJiaqian PuShuting HangManman LiuZiqi ChenJiayi XiongYongquan LiHongchen WuXiaodan ZhaoShuxun LiuQing GuPing LiThe gut microbiota is engaged in multiple interactions affecting host health. Bacteriocins showed the ability of impeding the growth of intestinal pathogenic bacteria and modulating gut microbiota in animals. Few studies have also discovered their regulation on human intestinal flora using an in vitro simulated system. However, little is known about their effect on gut microbiota of different enterotypes of human. This work evaluated the modification of the gut microbiota of two enterotypes (ET B and ET P) by the class IIb bacteriocin plantaricin NC8 (PLNC8) by using an in vitro fermentation model of the intestine. Gas chromatography results revealed that PLNC8 had no influence on the gut microbiota’s production of short-chain fatty acids in the subjects’ samples. PLNC8 lowered the Shannon index of ET B’ gut microbiota and the Simpson index of ET P’ gut microbiota, according to 16S rDNA sequencing. In ET B, PLNC8 enhanced the abundance of Bacteroides, Bifidobacterium, Megamonas, Escherichia-Shigella, Parabacteroides, and Lactobacillus while decreasing the abundance of Streptococcus. Prevotella_9, Bifidobacterium, Escherichia-Shigella, Mitsuokella, and Collinsella were found more abundant in ET P. The current study adds to our understanding of the impact of PLNC8 on the human gut microbiota and lays the groundwork for future research into PLNC8’s effects on human intestinal disease.https://www.frontiersin.org/articles/10.3389/fnut.2022.877948/fullbacteriocinplantaricin NC8in vitro fermentationgut microbiotashort-chain fatty acidsenterotypes |
| spellingShingle | Jiaqian Pu Shuting Hang Manman Liu Ziqi Chen Jiayi Xiong Yongquan Li Hongchen Wu Xiaodan Zhao Shuxun Liu Qing Gu Ping Li A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitro Frontiers in Nutrition bacteriocin plantaricin NC8 in vitro fermentation gut microbiota short-chain fatty acids enterotypes |
| title | A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitro |
| title_full | A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitro |
| title_fullStr | A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitro |
| title_full_unstemmed | A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitro |
| title_short | A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitro |
| title_sort | class iib bacteriocin plantaricin nc8 modulates gut microbiota of different enterotypes in vitro |
| topic | bacteriocin plantaricin NC8 in vitro fermentation gut microbiota short-chain fatty acids enterotypes |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.877948/full |
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