Mussels (Perna perna) as bioindicator of environmental contamination by Cryptosporidium species with zoonotic potential
Sources of contamination such as animal feces runoff, organic fertilizer application, and the release of partially treated or untreated sewage can lead to the contamination of aquatic environments by Cryptosporidium spp. The quality of mussels as food is closely related to the sanitary conditions of...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-04-01
|
| Series: | International Journal for Parasitology: Parasites and Wildlife |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213224416300049 |
| _version_ | 1819028033636401152 |
|---|---|
| author | Geisi Ferreira Mariné Oliveira Melissa Carvalho Machado do Couto Marcelo de Freitas Lima Teresa Cristina Bergamo do Bomfim |
| author_facet | Geisi Ferreira Mariné Oliveira Melissa Carvalho Machado do Couto Marcelo de Freitas Lima Teresa Cristina Bergamo do Bomfim |
| author_sort | Geisi Ferreira Mariné Oliveira |
| collection | DOAJ |
| description | Sources of contamination such as animal feces runoff, organic fertilizer application, and the release of partially treated or untreated sewage can lead to the contamination of aquatic environments by Cryptosporidium spp. The quality of mussels as food is closely related to the sanitary conditions of the marine environment where these bivalves are found. Marine mollusks are filter feeders that are able to retain Cryptosporidium oocysts in their tissue, thus functioning as bioindicators. A total of 72 pooled mussel samples of the species Perna perna were collected at two sites (A and B) in the municipality of Mangaratiba, Rio de Janeiro State, Brazil. Sampling involved removal of 30 mussels, from each collection site every month for one year. The 30 mussels from each sampling were then allocated into three groups of 10. Two Cryptosporidium spp. genes (18S and GP60) were targeted for DNA amplification from the samples obtained. After purification, all of the products obtained were sequenced and phylogenetic analyses were performed. Of the 72 samples analyzed using the nested-PCR for the 18S gene target, 29.2% were positive for the presence of Cryptosporidium spp. Of these samples, 52.4% were collected at site A (ie 11/21) and 47.6% at site B (ie 10/21). The 18S genes of all the samples considered positive for Cryptosporidium spp. were sequenced, and the following three species were identified: Cryptosporidium parvum, C. meleagridis, and C. andersoni. Three distinct C. parvum subtypes (IIaA19G2R2; IIaA20G2R2; IIaA20G3R2) were identified using the GP60 gene. More studies to evaluate the zoonotic potential of this species should be performed as both sampling locations contain human and/or animal fecal contaminants. |
| first_indexed | 2024-12-21T05:51:56Z |
| format | Article |
| id | doaj.art-6587ff44667a4657a2d359b6afaa4a16 |
| institution | Directory Open Access Journal |
| issn | 2213-2244 |
| language | English |
| last_indexed | 2024-12-21T05:51:56Z |
| publishDate | 2016-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal for Parasitology: Parasites and Wildlife |
| spelling | doaj.art-6587ff44667a4657a2d359b6afaa4a162022-12-21T19:13:57ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442016-04-0151283310.1016/j.ijppaw.2016.01.004Mussels (Perna perna) as bioindicator of environmental contamination by Cryptosporidium species with zoonotic potentialGeisi Ferreira Mariné Oliveira0Melissa Carvalho Machado do Couto1Marcelo de Freitas Lima2Teresa Cristina Bergamo do Bomfim3Educacional Foundation Rosemar Pimentel, University Center Geraldo Di Biase - Barra do Piraí, BrazilFederal Rural University of Rio de Janeiro, Institute of Biology, Department of Physiological Sciences, BrazilFederal Rural University of Rio de Janeiro – Chemistry Department, Institute of Exact Sciences, BrazilFederal Rural University of Rio de Janeiro, Veterinary Institute, Department of Animal Parasitology, BR 465, Km 07, Seropédica, Rio de Janeiro 23.890-000 BrazilSources of contamination such as animal feces runoff, organic fertilizer application, and the release of partially treated or untreated sewage can lead to the contamination of aquatic environments by Cryptosporidium spp. The quality of mussels as food is closely related to the sanitary conditions of the marine environment where these bivalves are found. Marine mollusks are filter feeders that are able to retain Cryptosporidium oocysts in their tissue, thus functioning as bioindicators. A total of 72 pooled mussel samples of the species Perna perna were collected at two sites (A and B) in the municipality of Mangaratiba, Rio de Janeiro State, Brazil. Sampling involved removal of 30 mussels, from each collection site every month for one year. The 30 mussels from each sampling were then allocated into three groups of 10. Two Cryptosporidium spp. genes (18S and GP60) were targeted for DNA amplification from the samples obtained. After purification, all of the products obtained were sequenced and phylogenetic analyses were performed. Of the 72 samples analyzed using the nested-PCR for the 18S gene target, 29.2% were positive for the presence of Cryptosporidium spp. Of these samples, 52.4% were collected at site A (ie 11/21) and 47.6% at site B (ie 10/21). The 18S genes of all the samples considered positive for Cryptosporidium spp. were sequenced, and the following three species were identified: Cryptosporidium parvum, C. meleagridis, and C. andersoni. Three distinct C. parvum subtypes (IIaA19G2R2; IIaA20G2R2; IIaA20G3R2) were identified using the GP60 gene. More studies to evaluate the zoonotic potential of this species should be performed as both sampling locations contain human and/or animal fecal contaminants.http://www.sciencedirect.com/science/article/pii/S2213224416300049Marine bivalveCryptosporidiumEnvironmental indicator18SGP60 |
| spellingShingle | Geisi Ferreira Mariné Oliveira Melissa Carvalho Machado do Couto Marcelo de Freitas Lima Teresa Cristina Bergamo do Bomfim Mussels (Perna perna) as bioindicator of environmental contamination by Cryptosporidium species with zoonotic potential International Journal for Parasitology: Parasites and Wildlife Marine bivalve Cryptosporidium Environmental indicator 18S GP60 |
| title | Mussels (Perna perna) as bioindicator of environmental contamination by Cryptosporidium species with zoonotic potential |
| title_full | Mussels (Perna perna) as bioindicator of environmental contamination by Cryptosporidium species with zoonotic potential |
| title_fullStr | Mussels (Perna perna) as bioindicator of environmental contamination by Cryptosporidium species with zoonotic potential |
| title_full_unstemmed | Mussels (Perna perna) as bioindicator of environmental contamination by Cryptosporidium species with zoonotic potential |
| title_short | Mussels (Perna perna) as bioindicator of environmental contamination by Cryptosporidium species with zoonotic potential |
| title_sort | mussels perna perna as bioindicator of environmental contamination by cryptosporidium species with zoonotic potential |
| topic | Marine bivalve Cryptosporidium Environmental indicator 18S GP60 |
| url | http://www.sciencedirect.com/science/article/pii/S2213224416300049 |
| work_keys_str_mv | AT geisiferreiramarineoliveira musselspernapernaasbioindicatorofenvironmentalcontaminationbycryptosporidiumspecieswithzoonoticpotential AT melissacarvalhomachadodocouto musselspernapernaasbioindicatorofenvironmentalcontaminationbycryptosporidiumspecieswithzoonoticpotential AT marcelodefreitaslima musselspernapernaasbioindicatorofenvironmentalcontaminationbycryptosporidiumspecieswithzoonoticpotential AT teresacristinabergamodobomfim musselspernapernaasbioindicatorofenvironmentalcontaminationbycryptosporidiumspecieswithzoonoticpotential |