A conservative approach in finding compatible blood for a patient with sickle cell disease having multiple alloantibodies
BACKGROUND: Sickle cell disease (SCD) patients may develop multiple alloantibodies that pose problem in finding compatible blood for transfusion and require crossmatching with large number of blood. AIM: The aim of the present study was to find compatible blood with reduced cost by adopting a conser...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Asian Journal of Transfusion Science |
| Subjects: | |
| Online Access: | http://www.ajts.org/article.asp?issn=0973-6247;year=2023;volume=17;issue=1;spage=121;epage=124;aulast=Joshi |
| _version_ | 1827986328012193792 |
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| author | Sanmukh Ratilal Joshi Mayuri M Vekariya Manisha M Rajapara |
| author_facet | Sanmukh Ratilal Joshi Mayuri M Vekariya Manisha M Rajapara |
| author_sort | Sanmukh Ratilal Joshi |
| collection | DOAJ |
| description | BACKGROUND: Sickle cell disease (SCD) patients may develop multiple alloantibodies that pose problem in finding compatible blood for transfusion and require crossmatching with large number of blood.
AIM: The aim of the present study was to find compatible blood with reduced cost by adopting a conservative approach.
MATERIALS AND METHODS: A step-by-step approach using tube technique, antibodies in original serum, and the saved test supernatant (TS) in search of compatible blood for transfusion purposes.
RESULTS: 32 years SCD patient grouped A with multiple antibodies required transfusion. A total of 641 red blood cell (RBC) units of groups A and O were crossmatched using serum and the TS by tube method. Of 138 units tested using the serum at 4°C, 124 units showed direct agglutination in the saline phase and the remaining 14 units were processed through low ionic strength solution (LISS)-IAT, of which 2 units were compatible even by the gel-IgG-card method. The TS, saved from the tests on serum, was used in an identical manner as that of the serum to screen additional 503 units by saline tube method at 4°C units showed direct agglutination of the RBCs of 428 units, hence were removed from inventory for this patient. The remaining 75 units were tested by the LISS-IAT-tube method at 37°C, of which 8 units were found compatible but only 2 units were clear compatible by the gel-IgG-card method. As such, 4 units compatible by the sensitive gel-IgG-card method were issued for transfusion purposes.
CONCLUSION: The new approach on using the saved TS consumed less of the patient's blood specimen, and the use of the tube method in screening and eliminating a large chunk of incompatible blood units has proved economical if compared with the use of the only gel-IgG-cards device in the entire maneuvering. |
| first_indexed | 2024-04-09T23:29:40Z |
| format | Article |
| id | doaj.art-65889dcf34de4a43bddbdb2040e4ef40 |
| institution | Directory Open Access Journal |
| issn | 0973-6247 1998-3565 |
| language | English |
| last_indexed | 2024-04-09T23:29:40Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Transfusion Science |
| spelling | doaj.art-65889dcf34de4a43bddbdb2040e4ef402023-03-21T07:27:37ZengWolters Kluwer Medknow PublicationsAsian Journal of Transfusion Science0973-62471998-35652023-01-0117112112410.4103/ajts.ajts_114_22A conservative approach in finding compatible blood for a patient with sickle cell disease having multiple alloantibodiesSanmukh Ratilal JoshiMayuri M VekariyaManisha M RajaparaBACKGROUND: Sickle cell disease (SCD) patients may develop multiple alloantibodies that pose problem in finding compatible blood for transfusion and require crossmatching with large number of blood. AIM: The aim of the present study was to find compatible blood with reduced cost by adopting a conservative approach. MATERIALS AND METHODS: A step-by-step approach using tube technique, antibodies in original serum, and the saved test supernatant (TS) in search of compatible blood for transfusion purposes. RESULTS: 32 years SCD patient grouped A with multiple antibodies required transfusion. A total of 641 red blood cell (RBC) units of groups A and O were crossmatched using serum and the TS by tube method. Of 138 units tested using the serum at 4°C, 124 units showed direct agglutination in the saline phase and the remaining 14 units were processed through low ionic strength solution (LISS)-IAT, of which 2 units were compatible even by the gel-IgG-card method. The TS, saved from the tests on serum, was used in an identical manner as that of the serum to screen additional 503 units by saline tube method at 4°C units showed direct agglutination of the RBCs of 428 units, hence were removed from inventory for this patient. The remaining 75 units were tested by the LISS-IAT-tube method at 37°C, of which 8 units were found compatible but only 2 units were clear compatible by the gel-IgG-card method. As such, 4 units compatible by the sensitive gel-IgG-card method were issued for transfusion purposes. CONCLUSION: The new approach on using the saved TS consumed less of the patient's blood specimen, and the use of the tube method in screening and eliminating a large chunk of incompatible blood units has proved economical if compared with the use of the only gel-IgG-cards device in the entire maneuvering.http://www.ajts.org/article.asp?issn=0973-6247;year=2023;volume=17;issue=1;spage=121;epage=124;aulast=Joshiconservative approachmultiple antibodiessickle cell disease |
| spellingShingle | Sanmukh Ratilal Joshi Mayuri M Vekariya Manisha M Rajapara A conservative approach in finding compatible blood for a patient with sickle cell disease having multiple alloantibodies Asian Journal of Transfusion Science conservative approach multiple antibodies sickle cell disease |
| title | A conservative approach in finding compatible blood for a patient with sickle cell disease having multiple alloantibodies |
| title_full | A conservative approach in finding compatible blood for a patient with sickle cell disease having multiple alloantibodies |
| title_fullStr | A conservative approach in finding compatible blood for a patient with sickle cell disease having multiple alloantibodies |
| title_full_unstemmed | A conservative approach in finding compatible blood for a patient with sickle cell disease having multiple alloantibodies |
| title_short | A conservative approach in finding compatible blood for a patient with sickle cell disease having multiple alloantibodies |
| title_sort | conservative approach in finding compatible blood for a patient with sickle cell disease having multiple alloantibodies |
| topic | conservative approach multiple antibodies sickle cell disease |
| url | http://www.ajts.org/article.asp?issn=0973-6247;year=2023;volume=17;issue=1;spage=121;epage=124;aulast=Joshi |
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