Effect of Ascaris lumbricoides infection on T helper cell type 2 in rural Egyptian children
Naglaa M Shalaby,1 Nehad M Shalaby2 1Department of Parasitology, Faculty of Medicine, 2Mansoura University Children Hospital, Mansoura University, Mansoura, Egypt Abstract: Ascaris lumbricoides is a neglected parasite that induces changes in host immune response. This study is conducted to define th...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2016-03-01
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| Series: | Therapeutics and Clinical Risk Management |
| Subjects: | |
| Online Access: | https://www.dovepress.com/effect-of-ascaris-lumbricoides-infection-on-t-helper-cell-type-2-in-ru-peer-reviewed-article-TCRM |
| _version_ | 1818824386826731520 |
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| author | Shalaby NM Shalaby NM |
| author_facet | Shalaby NM Shalaby NM |
| author_sort | Shalaby NM |
| collection | DOAJ |
| description | Naglaa M Shalaby,1 Nehad M Shalaby2 1Department of Parasitology, Faculty of Medicine, 2Mansoura University Children Hospital, Mansoura University, Mansoura, Egypt Abstract: Ascaris lumbricoides is a neglected parasite that induces changes in host immune response. This study is conducted to define the serum levels of tumor necrosis factor alpha (TNF-α), interleukin-4 (IL-4), and interleukin-5 (IL-5) in some Egyptian children and their relations to intensity of infection, age, and ascariasis symptoms. Stool samples were examined using formol-ether concentration and Kato-Katz thick smear techniques. Sera of 60 A. lumbricoides-infected children and 20 controls were tested by enzyme-linked immunosorbent assay. The mean sera concentrations of TNF-α, IL-4, and IL-5 were 7.41±2.5 pg/mL, 107.60±18.3 pg/mL, and 389.52±28.0 pg/mL, respectively. The controls had mean serum TNF-α 7.10±2.4 pg/mL, IL-4 25.49±2.6 pg/mL, and IL-5 88.76±22.7 pg/mL. The difference in the concentration of sera cytokines was statistically significant for IL-4 and IL-5 (P<0.01) between A. lumbricoides-infected children and controls. The intensity of infection correlated positively with IL-4 and IL-5 at r=0.959 and r=0.919, respectively. The concentrations of IL-4 and IL-5 correlated positively with the age at r=0.845 and r=0.934, respectively. Asthma and gastrointestinal tract upsets were correlated positively with IL-4 and IL-5. These data indicate that A. lumbricoides infection in our locality is associated with significantly high levels of IL-4 and IL-5.Keywords: Ascaris lumbricoides, children, rural, Egypt, IL-4, IL-5, TNF-α |
| first_indexed | 2024-12-18T23:55:03Z |
| format | Article |
| id | doaj.art-6588c1de9e1b46af9c600fc829270bee |
| institution | Directory Open Access Journal |
| issn | 1178-203X |
| language | English |
| last_indexed | 2024-12-18T23:55:03Z |
| publishDate | 2016-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Therapeutics and Clinical Risk Management |
| spelling | doaj.art-6588c1de9e1b46af9c600fc829270bee2022-12-21T20:46:43ZengDove Medical PressTherapeutics and Clinical Risk Management1178-203X2016-03-012016Issue 137938525928Effect of Ascaris lumbricoides infection on T helper cell type 2 in rural Egyptian childrenShalaby NMShalaby NMNaglaa M Shalaby,1 Nehad M Shalaby2 1Department of Parasitology, Faculty of Medicine, 2Mansoura University Children Hospital, Mansoura University, Mansoura, Egypt Abstract: Ascaris lumbricoides is a neglected parasite that induces changes in host immune response. This study is conducted to define the serum levels of tumor necrosis factor alpha (TNF-α), interleukin-4 (IL-4), and interleukin-5 (IL-5) in some Egyptian children and their relations to intensity of infection, age, and ascariasis symptoms. Stool samples were examined using formol-ether concentration and Kato-Katz thick smear techniques. Sera of 60 A. lumbricoides-infected children and 20 controls were tested by enzyme-linked immunosorbent assay. The mean sera concentrations of TNF-α, IL-4, and IL-5 were 7.41±2.5 pg/mL, 107.60±18.3 pg/mL, and 389.52±28.0 pg/mL, respectively. The controls had mean serum TNF-α 7.10±2.4 pg/mL, IL-4 25.49±2.6 pg/mL, and IL-5 88.76±22.7 pg/mL. The difference in the concentration of sera cytokines was statistically significant for IL-4 and IL-5 (P<0.01) between A. lumbricoides-infected children and controls. The intensity of infection correlated positively with IL-4 and IL-5 at r=0.959 and r=0.919, respectively. The concentrations of IL-4 and IL-5 correlated positively with the age at r=0.845 and r=0.934, respectively. Asthma and gastrointestinal tract upsets were correlated positively with IL-4 and IL-5. These data indicate that A. lumbricoides infection in our locality is associated with significantly high levels of IL-4 and IL-5.Keywords: Ascaris lumbricoides, children, rural, Egypt, IL-4, IL-5, TNF-αhttps://www.dovepress.com/effect-of-ascaris-lumbricoides-infection-on-t-helper-cell-type-2-in-ru-peer-reviewed-article-TCRMAscaris lumbricoideschildrenruralEgyptIL-4IL-5TNF-α |
| spellingShingle | Shalaby NM Shalaby NM Effect of Ascaris lumbricoides infection on T helper cell type 2 in rural Egyptian children Therapeutics and Clinical Risk Management Ascaris lumbricoides children rural Egypt IL-4 IL-5 TNF-α |
| title | Effect of Ascaris lumbricoides infection on T helper cell type 2 in rural Egyptian children |
| title_full | Effect of Ascaris lumbricoides infection on T helper cell type 2 in rural Egyptian children |
| title_fullStr | Effect of Ascaris lumbricoides infection on T helper cell type 2 in rural Egyptian children |
| title_full_unstemmed | Effect of Ascaris lumbricoides infection on T helper cell type 2 in rural Egyptian children |
| title_short | Effect of Ascaris lumbricoides infection on T helper cell type 2 in rural Egyptian children |
| title_sort | effect of ascaris lumbricoides infection on t helper cell type 2 in rural egyptian children |
| topic | Ascaris lumbricoides children rural Egypt IL-4 IL-5 TNF-α |
| url | https://www.dovepress.com/effect-of-ascaris-lumbricoides-infection-on-t-helper-cell-type-2-in-ru-peer-reviewed-article-TCRM |
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