Transcriptome Profiles Reveals <i>ScDREB10</i> from <i>Syntrichia caninervis</i> Regulated Phenylpropanoid Biosynthesis and Starch/Sucrose Metabolism to Enhance Plant Stress Tolerance
Desiccation is a kind of extreme form of drought stress and desiccation tolerance (DT) is an ancient trait of plants that allows them to survive tissue water potentials reaching −100 MPa or lower. <i>ScDREB10</i> is a DREB A-5 transcription factor gene from a DT moss named <i>Syntr...
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MDPI AG
2024-01-01
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| Series: | Plants |
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| author | Yuqing Liang Xiaoshuang Li Feiya Lei Ruirui Yang Wenwan Bai Qilin Yang Daoyuan Zhang |
| author_facet | Yuqing Liang Xiaoshuang Li Feiya Lei Ruirui Yang Wenwan Bai Qilin Yang Daoyuan Zhang |
| author_sort | Yuqing Liang |
| collection | DOAJ |
| description | Desiccation is a kind of extreme form of drought stress and desiccation tolerance (DT) is an ancient trait of plants that allows them to survive tissue water potentials reaching −100 MPa or lower. <i>ScDREB10</i> is a DREB A-5 transcription factor gene from a DT moss named <i>Syntrichia caninervis</i>, which has strong comprehensive tolerance to osmotic and salt stresses. This study delves further into the molecular mechanism of ScDREB10 stress tolerance based on the transcriptome data of the overexpression of <i>ScDREB10</i> in <i>Arabidopsis</i> under control, osmotic and salt treatments. The transcriptional analysis of weight gene co-expression network analysis (WGCNA) showed that “phenylpropanoid biosynthesis” and “starch and sucrose metabolism” were key pathways in the network of cyan and yellow modules. Meanwhile, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of differentially expressed genes (DEGs) also showed that “phenylpropanoid biosynthesis” and “starch and sucrose metabolism” pathways demonstrate the highest enrichment in response to osmotic and salt stress, respectively. Quantitative real-time PCR (qRT-PCR) results confirmed that most genes related to phenylpropanoid biosynthesis” and “starch and sucrose metabolism” pathways in overexpressing <i>ScDREB10 Arabidopsis</i> were up-regulated in response to osmotic and salt stresses, respectively. In line with the results, the corresponding lignin, sucrose, and trehalose contents and sucrose phosphate synthase activities were also increased in overexpressing ScDREB10 <i>Arabidopsis</i> under osmotic and salt stress treatments. Additionally, cis-acting promoter element analyses and yeast one-hybrid experiments showed that ScDREB10 was not only able to bind with classical cis-elements, such as DRE and TATCCC (MYBST1), but also bind with unknown element CGTCCA. All of these findings suggest that ScDREB10 may regulate plant stress tolerance by effecting phenylpropanoid biosynthesis, and starch and sucrose metabolism pathways. This research provides insights into the molecular mechanisms underpinning ScDREB10-mediated stress tolerance and contributes to deeply understanding the A-5 DREB regulatory mechanism. |
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| language | English |
| last_indexed | 2024-03-08T09:47:45Z |
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| spelling | doaj.art-658e20476d85411688038e87741bedbf2024-01-29T14:10:55ZengMDPI AGPlants2223-77472024-01-0113220510.3390/plants13020205Transcriptome Profiles Reveals <i>ScDREB10</i> from <i>Syntrichia caninervis</i> Regulated Phenylpropanoid Biosynthesis and Starch/Sucrose Metabolism to Enhance Plant Stress ToleranceYuqing Liang0Xiaoshuang Li1Feiya Lei2Ruirui Yang3Wenwan Bai4Qilin Yang5Daoyuan Zhang6State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, ChinaDesiccation is a kind of extreme form of drought stress and desiccation tolerance (DT) is an ancient trait of plants that allows them to survive tissue water potentials reaching −100 MPa or lower. <i>ScDREB10</i> is a DREB A-5 transcription factor gene from a DT moss named <i>Syntrichia caninervis</i>, which has strong comprehensive tolerance to osmotic and salt stresses. This study delves further into the molecular mechanism of ScDREB10 stress tolerance based on the transcriptome data of the overexpression of <i>ScDREB10</i> in <i>Arabidopsis</i> under control, osmotic and salt treatments. The transcriptional analysis of weight gene co-expression network analysis (WGCNA) showed that “phenylpropanoid biosynthesis” and “starch and sucrose metabolism” were key pathways in the network of cyan and yellow modules. Meanwhile, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of differentially expressed genes (DEGs) also showed that “phenylpropanoid biosynthesis” and “starch and sucrose metabolism” pathways demonstrate the highest enrichment in response to osmotic and salt stress, respectively. Quantitative real-time PCR (qRT-PCR) results confirmed that most genes related to phenylpropanoid biosynthesis” and “starch and sucrose metabolism” pathways in overexpressing <i>ScDREB10 Arabidopsis</i> were up-regulated in response to osmotic and salt stresses, respectively. In line with the results, the corresponding lignin, sucrose, and trehalose contents and sucrose phosphate synthase activities were also increased in overexpressing ScDREB10 <i>Arabidopsis</i> under osmotic and salt stress treatments. Additionally, cis-acting promoter element analyses and yeast one-hybrid experiments showed that ScDREB10 was not only able to bind with classical cis-elements, such as DRE and TATCCC (MYBST1), but also bind with unknown element CGTCCA. All of these findings suggest that ScDREB10 may regulate plant stress tolerance by effecting phenylpropanoid biosynthesis, and starch and sucrose metabolism pathways. This research provides insights into the molecular mechanisms underpinning ScDREB10-mediated stress tolerance and contributes to deeply understanding the A-5 DREB regulatory mechanism.https://www.mdpi.com/2223-7747/13/2/205AP2/ERF transcriptional factor<i>ScDREB10</i>phenylpropanoid biosynthesisstarch and sucrose metabolism<i>Syntrichia caninervis</i>plant stress tolerance |
| spellingShingle | Yuqing Liang Xiaoshuang Li Feiya Lei Ruirui Yang Wenwan Bai Qilin Yang Daoyuan Zhang Transcriptome Profiles Reveals <i>ScDREB10</i> from <i>Syntrichia caninervis</i> Regulated Phenylpropanoid Biosynthesis and Starch/Sucrose Metabolism to Enhance Plant Stress Tolerance Plants AP2/ERF transcriptional factor <i>ScDREB10</i> phenylpropanoid biosynthesis starch and sucrose metabolism <i>Syntrichia caninervis</i> plant stress tolerance |
| title | Transcriptome Profiles Reveals <i>ScDREB10</i> from <i>Syntrichia caninervis</i> Regulated Phenylpropanoid Biosynthesis and Starch/Sucrose Metabolism to Enhance Plant Stress Tolerance |
| title_full | Transcriptome Profiles Reveals <i>ScDREB10</i> from <i>Syntrichia caninervis</i> Regulated Phenylpropanoid Biosynthesis and Starch/Sucrose Metabolism to Enhance Plant Stress Tolerance |
| title_fullStr | Transcriptome Profiles Reveals <i>ScDREB10</i> from <i>Syntrichia caninervis</i> Regulated Phenylpropanoid Biosynthesis and Starch/Sucrose Metabolism to Enhance Plant Stress Tolerance |
| title_full_unstemmed | Transcriptome Profiles Reveals <i>ScDREB10</i> from <i>Syntrichia caninervis</i> Regulated Phenylpropanoid Biosynthesis and Starch/Sucrose Metabolism to Enhance Plant Stress Tolerance |
| title_short | Transcriptome Profiles Reveals <i>ScDREB10</i> from <i>Syntrichia caninervis</i> Regulated Phenylpropanoid Biosynthesis and Starch/Sucrose Metabolism to Enhance Plant Stress Tolerance |
| title_sort | transcriptome profiles reveals i scdreb10 i from i syntrichia caninervis i regulated phenylpropanoid biosynthesis and starch sucrose metabolism to enhance plant stress tolerance |
| topic | AP2/ERF transcriptional factor <i>ScDREB10</i> phenylpropanoid biosynthesis starch and sucrose metabolism <i>Syntrichia caninervis</i> plant stress tolerance |
| url | https://www.mdpi.com/2223-7747/13/2/205 |
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