The Effect of Resistance Training on the Expression of Cardiac Muscle Growth Regulator Messenger Genes in Obese Male Rats
Background & aim: Obesity is associated with cardiovascular disease and may lead to pathological cardiac hypertrophy, but physical activity (resistance training) modulates some intracellular signaling pathways associated with the regulation of hypertrophy. The aim of the present study was to det...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
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Yasuj University Of Medical Sciences
2019-12-01
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| Series: | Armaghane Danesh Bimonthly Journal |
| Subjects: | |
| Online Access: | http://armaghanj.yums.ac.ir/article-1-2570-en.html |
| _version_ | 1811236121316687872 |
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| author | A Moini S Farsi SA Hoseini M Mehrzad |
| author_facet | A Moini S Farsi SA Hoseini M Mehrzad |
| author_sort | A Moini |
| collection | DOAJ |
| description | Background & aim: Obesity is associated with cardiovascular disease and may lead to pathological cardiac hypertrophy, but physical activity (resistance training) modulates some intracellular signaling pathways associated with the regulation of hypertrophy. The aim of the present study was to determine and evaluate the effect of resistance training on the expression of cardiovascular regulatory genes in obese male rats.
Methods: In the present experimental study which was conducted in 2016, 18 male Sprague Dawley rats were divided into 3 groups of healthy control, obesity control and resistance training, after eight weeks of using high fat diet. The rats were dissected 48 hours after exercise protocol and their heart muscle was separated. The expression of genes (AMPK, mTOR, S6K, 4EBP, COL1, COL3, AngII) was determined by Real time-PCR technique. The amount of transgene was calculated by CT-2ΔΔ method. Data were analyzed by statistical tests. Independent t-test, Kolmogorov-Smirnov and one-way ANOVA were used for data analysis.
Results: The results indicated that the resistance training group compared to the control group showed an increase in expression of mTOR(p=0.003), 4EBP (p=0.004), which was not statistically significant. On the other hand, expression of S6K (p=0.002) AMPK (p=0.008) increased significantly. Also, there was a decrease in the expression of collagen 1 (p=0.003), collagen 3 (p=0.001) Ang II (p=0.001) which were statistically significant.
Conclusion: Obesity in rats seemed to induce cardiac pathological hypertrophy and accelerate the process of cardiac senescence, but resistance training, which was a suitable replacement, partially reduced the negative effects of obesity on cardiac function in the process of cardiac senescence. |
| first_indexed | 2024-04-12T12:03:29Z |
| format | Article |
| id | doaj.art-65916cc672274231aec914de9bb0f81b |
| institution | Directory Open Access Journal |
| issn | 1728-6514 1728-6514 |
| language | fas |
| last_indexed | 2024-04-12T12:03:29Z |
| publishDate | 2019-12-01 |
| publisher | Yasuj University Of Medical Sciences |
| record_format | Article |
| series | Armaghane Danesh Bimonthly Journal |
| spelling | doaj.art-65916cc672274231aec914de9bb0f81b2022-12-22T03:33:47ZfasYasuj University Of Medical SciencesArmaghane Danesh Bimonthly Journal1728-65141728-65142019-12-01245935949The Effect of Resistance Training on the Expression of Cardiac Muscle Growth Regulator Messenger Genes in Obese Male RatsA Moini0S Farsi1SA Hoseini2M Mehrzad3 Department of Physical Education and Sport Sciences, Larestan Branch, Islamic Azad University, Shiraz, Iran, Department of Physical Education and Sport Sciences, Larestan Branch, Islamic Azad University, Shiraz, Iran, Department of Exercises Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran Department of Physical Education, Shiraz Branch, Islamic Azad University, Shiraz, Iran. Background & aim: Obesity is associated with cardiovascular disease and may lead to pathological cardiac hypertrophy, but physical activity (resistance training) modulates some intracellular signaling pathways associated with the regulation of hypertrophy. The aim of the present study was to determine and evaluate the effect of resistance training on the expression of cardiovascular regulatory genes in obese male rats. Methods: In the present experimental study which was conducted in 2016, 18 male Sprague Dawley rats were divided into 3 groups of healthy control, obesity control and resistance training, after eight weeks of using high fat diet. The rats were dissected 48 hours after exercise protocol and their heart muscle was separated. The expression of genes (AMPK, mTOR, S6K, 4EBP, COL1, COL3, AngII) was determined by Real time-PCR technique. The amount of transgene was calculated by CT-2ΔΔ method. Data were analyzed by statistical tests. Independent t-test, Kolmogorov-Smirnov and one-way ANOVA were used for data analysis. Results: The results indicated that the resistance training group compared to the control group showed an increase in expression of mTOR(p=0.003), 4EBP (p=0.004), which was not statistically significant. On the other hand, expression of S6K (p=0.002) AMPK (p=0.008) increased significantly. Also, there was a decrease in the expression of collagen 1 (p=0.003), collagen 3 (p=0.001) Ang II (p=0.001) which were statistically significant. Conclusion: Obesity in rats seemed to induce cardiac pathological hypertrophy and accelerate the process of cardiac senescence, but resistance training, which was a suitable replacement, partially reduced the negative effects of obesity on cardiac function in the process of cardiac senescence.http://armaghanj.yums.ac.ir/article-1-2570-en.htmlcardiac muscleobesityresistance training. |
| spellingShingle | A Moini S Farsi SA Hoseini M Mehrzad The Effect of Resistance Training on the Expression of Cardiac Muscle Growth Regulator Messenger Genes in Obese Male Rats Armaghane Danesh Bimonthly Journal cardiac muscle obesity resistance training. |
| title | The Effect of Resistance Training on the Expression of Cardiac Muscle Growth Regulator Messenger Genes in Obese Male Rats |
| title_full | The Effect of Resistance Training on the Expression of Cardiac Muscle Growth Regulator Messenger Genes in Obese Male Rats |
| title_fullStr | The Effect of Resistance Training on the Expression of Cardiac Muscle Growth Regulator Messenger Genes in Obese Male Rats |
| title_full_unstemmed | The Effect of Resistance Training on the Expression of Cardiac Muscle Growth Regulator Messenger Genes in Obese Male Rats |
| title_short | The Effect of Resistance Training on the Expression of Cardiac Muscle Growth Regulator Messenger Genes in Obese Male Rats |
| title_sort | effect of resistance training on the expression of cardiac muscle growth regulator messenger genes in obese male rats |
| topic | cardiac muscle obesity resistance training. |
| url | http://armaghanj.yums.ac.ir/article-1-2570-en.html |
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