Luteal Function, Biometrics, and Echotextural Attributes in Santa Inês Ewes Superovulated with Different Total Doses of Porcine Follicle-Stimulating Hormone
Premature regression of corpora lutea (PRCL) may adversely affect the outcome of hormonal ovarian superstimulation in small ruminants, and the total dose of exogenous gonadotropins used may be one of the causes of this condition. There were two major objectives of the present study: (1) to evaluate...
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MDPI AG
2023-02-01
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| author | Júlia Ribeiro Bevilaqua Mariana Garcia Kako Rodriguez Giovanna Serpa Maciel Gabriel Brun Vergani Jeferson Ferreira da Fonseca Pawel Mieczyslaw Bartlewski Maria Emilia Franco Oliveira |
| author_facet | Júlia Ribeiro Bevilaqua Mariana Garcia Kako Rodriguez Giovanna Serpa Maciel Gabriel Brun Vergani Jeferson Ferreira da Fonseca Pawel Mieczyslaw Bartlewski Maria Emilia Franco Oliveira |
| author_sort | Júlia Ribeiro Bevilaqua |
| collection | DOAJ |
| description | Premature regression of corpora lutea (PRCL) may adversely affect the outcome of hormonal ovarian superstimulation in small ruminants, and the total dose of exogenous gonadotropins used may be one of the causes of this condition. There were two major objectives of the present study: (1) to evaluate the effects of different superovulatory doses of porcine follicle-stimulating hormone (pFSH) on the biometry, blood perfusion (Doppler), and echotextural characteristics of luteal structures; and, (2) to determine the usefulness of biometric, vascular, and echotextural luteal variables, as well as measurements of circulating progesterone (P<sub>4</sub>) concentrations for early detection of PRCL in superovulated Santa Inês ewes. Twenty-seven Santa Inês ewes received an intravaginal P<sub>4</sub>-releasing device (CIDR) from Days 0 to 8 (Day 0 = random day of the anovulatory period). An IM injection of d-cloprostenol (37.5 μg) was given at the time of the CIDR insertion and withdrawal. On Day 6, all the ewes received 300 IU of eCG IM and were divided into three treatment groups (each <i>n</i> = 9): G100 (100 mg); G133 (133 mg); and G200 (200 mg of pFSH) administered IM every 12 h in eight injections. Transrectal ovarian ultrasonography and jugular blood sampling for serum P<sub>4</sub> measurements were performed on Days 11 to 15. On the day of embryo recovery (Day 15), all the ewes underwent diagnostic videolaparoscopy and were classified, based on their luteal characteristics, into three response groups: nCL (ewes with normal CL only); rCL (ewes with regressing CL only); and ewes with both nCL and rCL following the superovulatory regimen. Our present results indicate that the total pFSH doses of 100 mg and 200 mg result in similar ovulatory responses and luteal function/biometrics, although the percentage of donor ewes with nCL was greater (<i>p</i> < 0.05) for G100 compared with the G200 animals. An application of 133 mg of pFSH was associated with diminished luteogenesis. Lastly, circulating P<sub>4</sub> concentrations, ultrasonographic estimates of total luteal area, and CL pixel heterogeneity (standard deviation of numerical pixel values) are promising markers of luteal inadequacy in superovulated ewes. |
| first_indexed | 2024-03-11T07:31:49Z |
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| spelling | doaj.art-65925bffcc724ebe96fe1cea015e4a872023-11-17T07:13:32ZengMDPI AGAnimals2076-26152023-02-0113587310.3390/ani13050873Luteal Function, Biometrics, and Echotextural Attributes in Santa Inês Ewes Superovulated with Different Total Doses of Porcine Follicle-Stimulating HormoneJúlia Ribeiro Bevilaqua0Mariana Garcia Kako Rodriguez1Giovanna Serpa Maciel2Gabriel Brun Vergani3Jeferson Ferreira da Fonseca4Pawel Mieczyslaw Bartlewski5Maria Emilia Franco Oliveira6Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University, Jaboticabal 14884-900, SP, BrazilDepartment of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University, Jaboticabal 14884-900, SP, BrazilDepartment of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University, Jaboticabal 14884-900, SP, BrazilDepartment of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University, Jaboticabal 14884-900, SP, BrazilEmbrapa Goats and Sheep, Coronel Pacheco 36155-000, MG, BrazilDepartment of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, CanadaDepartment of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University, Jaboticabal 14884-900, SP, BrazilPremature regression of corpora lutea (PRCL) may adversely affect the outcome of hormonal ovarian superstimulation in small ruminants, and the total dose of exogenous gonadotropins used may be one of the causes of this condition. There were two major objectives of the present study: (1) to evaluate the effects of different superovulatory doses of porcine follicle-stimulating hormone (pFSH) on the biometry, blood perfusion (Doppler), and echotextural characteristics of luteal structures; and, (2) to determine the usefulness of biometric, vascular, and echotextural luteal variables, as well as measurements of circulating progesterone (P<sub>4</sub>) concentrations for early detection of PRCL in superovulated Santa Inês ewes. Twenty-seven Santa Inês ewes received an intravaginal P<sub>4</sub>-releasing device (CIDR) from Days 0 to 8 (Day 0 = random day of the anovulatory period). An IM injection of d-cloprostenol (37.5 μg) was given at the time of the CIDR insertion and withdrawal. On Day 6, all the ewes received 300 IU of eCG IM and were divided into three treatment groups (each <i>n</i> = 9): G100 (100 mg); G133 (133 mg); and G200 (200 mg of pFSH) administered IM every 12 h in eight injections. Transrectal ovarian ultrasonography and jugular blood sampling for serum P<sub>4</sub> measurements were performed on Days 11 to 15. On the day of embryo recovery (Day 15), all the ewes underwent diagnostic videolaparoscopy and were classified, based on their luteal characteristics, into three response groups: nCL (ewes with normal CL only); rCL (ewes with regressing CL only); and ewes with both nCL and rCL following the superovulatory regimen. Our present results indicate that the total pFSH doses of 100 mg and 200 mg result in similar ovulatory responses and luteal function/biometrics, although the percentage of donor ewes with nCL was greater (<i>p</i> < 0.05) for G100 compared with the G200 animals. An application of 133 mg of pFSH was associated with diminished luteogenesis. Lastly, circulating P<sub>4</sub> concentrations, ultrasonographic estimates of total luteal area, and CL pixel heterogeneity (standard deviation of numerical pixel values) are promising markers of luteal inadequacy in superovulated ewes.https://www.mdpi.com/2076-2615/13/5/873superovulationcorpus luteumprogesteroneovarian ultrasonographysheep |
| spellingShingle | Júlia Ribeiro Bevilaqua Mariana Garcia Kako Rodriguez Giovanna Serpa Maciel Gabriel Brun Vergani Jeferson Ferreira da Fonseca Pawel Mieczyslaw Bartlewski Maria Emilia Franco Oliveira Luteal Function, Biometrics, and Echotextural Attributes in Santa Inês Ewes Superovulated with Different Total Doses of Porcine Follicle-Stimulating Hormone Animals superovulation corpus luteum progesterone ovarian ultrasonography sheep |
| title | Luteal Function, Biometrics, and Echotextural Attributes in Santa Inês Ewes Superovulated with Different Total Doses of Porcine Follicle-Stimulating Hormone |
| title_full | Luteal Function, Biometrics, and Echotextural Attributes in Santa Inês Ewes Superovulated with Different Total Doses of Porcine Follicle-Stimulating Hormone |
| title_fullStr | Luteal Function, Biometrics, and Echotextural Attributes in Santa Inês Ewes Superovulated with Different Total Doses of Porcine Follicle-Stimulating Hormone |
| title_full_unstemmed | Luteal Function, Biometrics, and Echotextural Attributes in Santa Inês Ewes Superovulated with Different Total Doses of Porcine Follicle-Stimulating Hormone |
| title_short | Luteal Function, Biometrics, and Echotextural Attributes in Santa Inês Ewes Superovulated with Different Total Doses of Porcine Follicle-Stimulating Hormone |
| title_sort | luteal function biometrics and echotextural attributes in santa ines ewes superovulated with different total doses of porcine follicle stimulating hormone |
| topic | superovulation corpus luteum progesterone ovarian ultrasonography sheep |
| url | https://www.mdpi.com/2076-2615/13/5/873 |
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