Insights into the dynamics of hind leg development in honey bee (Apis mellifera L.) queen and worker larvae - A morphology/differential gene expression analysis
Phenotypic plasticity is a hallmark of the caste systems of social insects, expressed in their life history and morphological traits. These are best studied in bees. In their co-evolution with angiosperm plants, the females of corbiculate bees have acquired a specialized structure on their hind legs...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2015-09-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300263&lng=en&tlng=en |
| _version_ | 1811215567275687936 |
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| author | Carolina Gonçalves Santos Klaus Hartfelder |
| author_facet | Carolina Gonçalves Santos Klaus Hartfelder |
| author_sort | Carolina Gonçalves Santos |
| collection | DOAJ |
| description | Phenotypic plasticity is a hallmark of the caste systems of social insects, expressed in their life history and morphological traits. These are best studied in bees. In their co-evolution with angiosperm plants, the females of corbiculate bees have acquired a specialized structure on their hind legs for collecting pollen. In the highly eusocial bees (Apini and Meliponini), this structure is however only present in workers and absent in queens. By means of histological sections and cell proliferation analysis we followed the developmental dynamics of the hind legs of queens and workers in the fourth and fifth larval instars. In parallel, we generated subtractive cDNA libraries for hind leg discs of queen and worker larvae by means of a Representational Difference Analysis (RDA). From the total of 135 unique sequences we selected 19 for RT-qPCR analysis, where six of these were confirmed as differing significantly in their expression between the two castes in the larval spinning stage. The development of complex structures such as the bees’ hind legs, requires diverse patterning mechanisms and signaling modules, as indicated by the set of differentially expressed genes related with cell adhesion and signaling pathways. |
| first_indexed | 2024-04-12T06:24:42Z |
| format | Article |
| id | doaj.art-659412e852d74ebc8467fd41ee6f9e52 |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-04-12T06:24:42Z |
| publishDate | 2015-09-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-659412e852d74ebc8467fd41ee6f9e522022-12-22T03:44:11ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852015-09-0138326327710.1590/S1415-475738320140393S1415-47572015000300263Insights into the dynamics of hind leg development in honey bee (Apis mellifera L.) queen and worker larvae - A morphology/differential gene expression analysisCarolina Gonçalves SantosKlaus HartfelderPhenotypic plasticity is a hallmark of the caste systems of social insects, expressed in their life history and morphological traits. These are best studied in bees. In their co-evolution with angiosperm plants, the females of corbiculate bees have acquired a specialized structure on their hind legs for collecting pollen. In the highly eusocial bees (Apini and Meliponini), this structure is however only present in workers and absent in queens. By means of histological sections and cell proliferation analysis we followed the developmental dynamics of the hind legs of queens and workers in the fourth and fifth larval instars. In parallel, we generated subtractive cDNA libraries for hind leg discs of queen and worker larvae by means of a Representational Difference Analysis (RDA). From the total of 135 unique sequences we selected 19 for RT-qPCR analysis, where six of these were confirmed as differing significantly in their expression between the two castes in the larval spinning stage. The development of complex structures such as the bees’ hind legs, requires diverse patterning mechanisms and signaling modules, as indicated by the set of differentially expressed genes related with cell adhesion and signaling pathways.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300263&lng=en&tlng=ensocial insectcaste developmentimaginal discinsect metamorphosistranscript analysis |
| spellingShingle | Carolina Gonçalves Santos Klaus Hartfelder Insights into the dynamics of hind leg development in honey bee (Apis mellifera L.) queen and worker larvae - A morphology/differential gene expression analysis Genetics and Molecular Biology social insect caste development imaginal disc insect metamorphosis transcript analysis |
| title | Insights into the dynamics of hind leg development in honey bee (Apis mellifera L.) queen and worker larvae - A morphology/differential gene expression analysis |
| title_full | Insights into the dynamics of hind leg development in honey bee (Apis mellifera L.) queen and worker larvae - A morphology/differential gene expression analysis |
| title_fullStr | Insights into the dynamics of hind leg development in honey bee (Apis mellifera L.) queen and worker larvae - A morphology/differential gene expression analysis |
| title_full_unstemmed | Insights into the dynamics of hind leg development in honey bee (Apis mellifera L.) queen and worker larvae - A morphology/differential gene expression analysis |
| title_short | Insights into the dynamics of hind leg development in honey bee (Apis mellifera L.) queen and worker larvae - A morphology/differential gene expression analysis |
| title_sort | insights into the dynamics of hind leg development in honey bee apis mellifera l queen and worker larvae a morphology differential gene expression analysis |
| topic | social insect caste development imaginal disc insect metamorphosis transcript analysis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300263&lng=en&tlng=en |
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