A grooved conduit combined with decellularized tissues for peripheral nerve regeneration
Abstract Peripheral nerve injury (PNI) is a common and severe clinical disease worldwide, which leads to a poor prognosis because of the complicated treatments and high morbidity. Autologous nerve grafting as the gold standard still cannot meet the needs of clinical nerve transplantation because of...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2023-07-01
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| Series: | Journal of Materials Science: Materials in Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s10856-023-06737-z |
| _version_ | 1797752837341446144 |
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| author | Enxing Yu Zhiwu Chen Yuye Huang Yibing Wu Zonghuan Wang Fangfang Wang Miaoben Wu Kailei Xu Wei Peng |
| author_facet | Enxing Yu Zhiwu Chen Yuye Huang Yibing Wu Zonghuan Wang Fangfang Wang Miaoben Wu Kailei Xu Wei Peng |
| author_sort | Enxing Yu |
| collection | DOAJ |
| description | Abstract Peripheral nerve injury (PNI) is a common and severe clinical disease worldwide, which leads to a poor prognosis because of the complicated treatments and high morbidity. Autologous nerve grafting as the gold standard still cannot meet the needs of clinical nerve transplantation because of its low availability and limited size. The development of artificial nerve conduits was led to a novel direction for PNI treatment, while most of the currently developed artificial nerve conduits was lack biochemical cues to promote nerve regeneration. In this study, we designed a novel composite neural conduit by inserting decellularized the rat sciatic nerve or kidney in a poly (lactic-co-glycolic acid) (PLGA) grooved conduit. The nerve regeneration effect of all samples was analyzed using rat sciatic nerve defect model, where decellularized tissues and grooved PLGA conduit alone were used as controls. The degree of nerve regeneration was evaluated using the motor function, gastrocnemius recovery, and morphological and histological assessments suggested that the combination of a grooved conduit with decellularized tissues significantly promoted nerve regeneration compared with decellularized tissues and PLGA conduit alone. It is worth to note that the grooved conduits containing decellularized nerves have a promotive effect similar to that of autologous nerve grafting, suggesting that it could be an artificial nerve conduit used for clinical practice in the future. Graphical Abstract |
| first_indexed | 2024-03-12T17:10:16Z |
| format | Article |
| id | doaj.art-659458fda9c54b059ee1d0ebf10b86e7 |
| institution | Directory Open Access Journal |
| issn | 1573-4838 |
| language | English |
| last_indexed | 2024-03-12T17:10:16Z |
| publishDate | 2023-07-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj.art-659458fda9c54b059ee1d0ebf10b86e72023-08-06T11:05:04ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382023-07-0134711510.1007/s10856-023-06737-zA grooved conduit combined with decellularized tissues for peripheral nerve regenerationEnxing Yu0Zhiwu Chen1Yuye Huang2Yibing Wu3Zonghuan Wang4Fangfang Wang5Miaoben Wu6Kailei Xu7Wei Peng8Department of Plastic and reconstructive surgery, The First Affiliated Hospital, Ningbo University School of MedicineDepartment of Plastic and reconstructive surgery, The First Affiliated Hospital, Ningbo University School of MedicineCenter for Medical and Engineering Innovation, Central Laboratory, The First Affiliated Hospital, Ningbo University School of MedicineDepartment of Plastic and reconstructive surgery, The First Affiliated Hospital, Ningbo University School of MedicineCentral Laboratory, The First Affiliated Hospital, Ningbo University School of MedicineCentral Laboratory, The First Affiliated Hospital, Ningbo University School of MedicineSchool of Medicine, Ningbo UniversityDepartment of Plastic and reconstructive surgery, The First Affiliated Hospital, Ningbo University School of MedicineDepartment of Plastic and reconstructive surgery, The First Affiliated Hospital, Ningbo University School of MedicineAbstract Peripheral nerve injury (PNI) is a common and severe clinical disease worldwide, which leads to a poor prognosis because of the complicated treatments and high morbidity. Autologous nerve grafting as the gold standard still cannot meet the needs of clinical nerve transplantation because of its low availability and limited size. The development of artificial nerve conduits was led to a novel direction for PNI treatment, while most of the currently developed artificial nerve conduits was lack biochemical cues to promote nerve regeneration. In this study, we designed a novel composite neural conduit by inserting decellularized the rat sciatic nerve or kidney in a poly (lactic-co-glycolic acid) (PLGA) grooved conduit. The nerve regeneration effect of all samples was analyzed using rat sciatic nerve defect model, where decellularized tissues and grooved PLGA conduit alone were used as controls. The degree of nerve regeneration was evaluated using the motor function, gastrocnemius recovery, and morphological and histological assessments suggested that the combination of a grooved conduit with decellularized tissues significantly promoted nerve regeneration compared with decellularized tissues and PLGA conduit alone. It is worth to note that the grooved conduits containing decellularized nerves have a promotive effect similar to that of autologous nerve grafting, suggesting that it could be an artificial nerve conduit used for clinical practice in the future. Graphical Abstracthttps://doi.org/10.1007/s10856-023-06737-zPeripheral nerve injuryComposite nerve conduitPLGADecellularized tissueNerve repairTissue engineering |
| spellingShingle | Enxing Yu Zhiwu Chen Yuye Huang Yibing Wu Zonghuan Wang Fangfang Wang Miaoben Wu Kailei Xu Wei Peng A grooved conduit combined with decellularized tissues for peripheral nerve regeneration Journal of Materials Science: Materials in Medicine Peripheral nerve injury Composite nerve conduit PLGA Decellularized tissue Nerve repair Tissue engineering |
| title | A grooved conduit combined with decellularized tissues for peripheral nerve regeneration |
| title_full | A grooved conduit combined with decellularized tissues for peripheral nerve regeneration |
| title_fullStr | A grooved conduit combined with decellularized tissues for peripheral nerve regeneration |
| title_full_unstemmed | A grooved conduit combined with decellularized tissues for peripheral nerve regeneration |
| title_short | A grooved conduit combined with decellularized tissues for peripheral nerve regeneration |
| title_sort | grooved conduit combined with decellularized tissues for peripheral nerve regeneration |
| topic | Peripheral nerve injury Composite nerve conduit PLGA Decellularized tissue Nerve repair Tissue engineering |
| url | https://doi.org/10.1007/s10856-023-06737-z |
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