Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE
This study was carried out to determine the whole cell protein profiles of Salmonella serovars from chicken, turkey and sheep faeces by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A total of 34 Salmonella strains were included in the study, 14 of them were isolated from chi...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2010-06-01
|
| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-201006-0001_analysis-of-whole-cell-protein-profiles-of-salmonella-serovars-isolated-from-chicken-turkey-and-sheep-faeces-b.php |
| _version_ | 1797897661582409728 |
|---|---|
| author | A. Aksakal |
| author_facet | A. Aksakal |
| author_sort | A. Aksakal |
| collection | DOAJ |
| description | This study was carried out to determine the whole cell protein profiles of Salmonella serovars from chicken, turkey and sheep faeces by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A total of 34 Salmonella strains were included in the study, 14 of them were isolated from chicken, 14 from turkey and six from sheep. SDS-PAGE was carried out using 12% (w/v) separating and 4% (w/v) stacking gels. The results showed more than 30 protein bands ranging in size from 97 kDa (kilodaltons) to below 14.4 kDa as determined by visual assessment of their approximate molecular masses. Protein bands of 78.1, 51.2, 41.5, 37.3, 35.1, 33.9, 30.7, 27.6, 25.4, and 24 kDa were detected in all Salmonella serovars. Salmonella strains used in this study were closely related and could not be differentiated depending on the whole cell protein profiles using SDS-PAGE. |
| first_indexed | 2024-04-10T08:00:44Z |
| format | Article |
| id | doaj.art-659459f17e924f65871e0a3dc8cf64da |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:00:44Z |
| publishDate | 2010-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-659459f17e924f65871e0a3dc8cf64da2023-02-23T03:49:48ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922010-06-0155625926310.17221/2986-VETMEDvet-201006-0001Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGEA. Aksakal0Faculty of Veterinary Science, Yuzuncu Yil University, Van, TurkeyThis study was carried out to determine the whole cell protein profiles of Salmonella serovars from chicken, turkey and sheep faeces by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A total of 34 Salmonella strains were included in the study, 14 of them were isolated from chicken, 14 from turkey and six from sheep. SDS-PAGE was carried out using 12% (w/v) separating and 4% (w/v) stacking gels. The results showed more than 30 protein bands ranging in size from 97 kDa (kilodaltons) to below 14.4 kDa as determined by visual assessment of their approximate molecular masses. Protein bands of 78.1, 51.2, 41.5, 37.3, 35.1, 33.9, 30.7, 27.6, 25.4, and 24 kDa were detected in all Salmonella serovars. Salmonella strains used in this study were closely related and could not be differentiated depending on the whole cell protein profiles using SDS-PAGE.https://vetmed.agriculturejournals.cz/artkey/vet-201006-0001_analysis-of-whole-cell-protein-profiles-of-salmonella-serovars-isolated-from-chicken-turkey-and-sheep-faeces-b.phpwhole cell proteinsalmonellachickenturkeysheepsds-page |
| spellingShingle | A. Aksakal Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE Veterinární Medicína whole cell protein salmonella chicken turkey sheep sds-page |
| title | Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE |
| title_full | Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE |
| title_fullStr | Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE |
| title_full_unstemmed | Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE |
| title_short | Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE |
| title_sort | analysis of whole cell protein profiles of salmonella serovars isolated from chicken turkey and sheep faeces by sds page |
| topic | whole cell protein salmonella chicken turkey sheep sds-page |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-201006-0001_analysis-of-whole-cell-protein-profiles-of-salmonella-serovars-isolated-from-chicken-turkey-and-sheep-faeces-b.php |
| work_keys_str_mv | AT aaksakal analysisofwholecellproteinprofilesofsalmonellaserovarsisolatedfromchickenturkeyandsheepfaecesbysdspage |