Isolation and characterization of a sequence type 25 carbapenem-resistant hypervirulent Klebsiella pneumoniae from the mid-south region of China
Abstract Background The molecular characterization of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) isolates is not well studied. Our goal was to investigate the molecular epidemiology of CR-hvKP strains that were isolated from a Chinese hospital. Results All clinical carbapenem...
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| Format: | Article |
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BMC
2019-09-01
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| Series: | BMC Microbiology |
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| Online Access: | http://link.springer.com/article/10.1186/s12866-019-1593-5 |
| _version_ | 1819203298842902528 |
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| author | Jun Li Zi-Yan Huang Ting Yu Xiao-Yan Tao Yong-Mei Hu Hai-Chen Wang Ming-Xiang Zou |
| author_facet | Jun Li Zi-Yan Huang Ting Yu Xiao-Yan Tao Yong-Mei Hu Hai-Chen Wang Ming-Xiang Zou |
| author_sort | Jun Li |
| collection | DOAJ |
| description | Abstract Background The molecular characterization of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) isolates is not well studied. Our goal was to investigate the molecular epidemiology of CR-hvKP strains that were isolated from a Chinese hospital. Results All clinical carbapenem-resistant K. pneumoniae (CR-KP) isolates were collected and identified from patient samples between 2014 and 2017 from a Chinese hospital. The samples were subjected to screening for CR-hvKP by string test and the detection of the aerobactin gene. CR-hvKP isolates were further confirmed through neutrophil phagocytosis and a mice lethality assay. The CR-hvKP isolates were investigated for their capsular genotyping, virulence gene profiles, and the expression of carbapenemase genes by PCR and DNA sequencing. Multilocus sequence type (MLST) and pulsed-field gel electrophoresis (PFGE) were performed to exclude the homology of these isolates. Twenty strains were identified as CR-hvKP. These strains were resistant to imipenem and several other antibiotics, however, most were susceptible to amikacin. Notably, two isolates were not susceptible to tigecycline. Capsular polysaccharide synthesis genotyping revealed that 17 of the 20 CR-hvKP strains belonged to the K2 serotype, while the others belonged to serotypes other than K1, K2, K5, K20, and K57. The strains were found to be positive for 10 types of virulence genes and a variety of these genes coexisted in the same strain. Two carbapenemase genes were identified: bla KPC-2 (13/20) and bla NDM-1 (1/20). PFGE typing revealed eight clusters comprising isolates that belonged to MLST types ST25, ST11 and ST375, respectively. PFGE cluster A was identified as the main cluster, which included 11 isolates that belong to ST25 and mainly from ICU department. Conclusions Our findings suggest that hospital-acquired infections may contribute in part to the CR-hvKP strains identified in this study. It also suggests that ST25 CR-hvKP strain has a clonal distribution in our hospital. Therefore, effective surveillance and strict infection control strategies should be implemented to prevent outbreak by CR-hvKP strains in hospitals setting. |
| first_indexed | 2024-12-23T04:17:42Z |
| format | Article |
| id | doaj.art-6594bed5526b4324a696e8f70b33ed14 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-23T04:17:42Z |
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| spelling | doaj.art-6594bed5526b4324a696e8f70b33ed142022-12-21T18:00:21ZengBMCBMC Microbiology1471-21802019-09-0119111010.1186/s12866-019-1593-5Isolation and characterization of a sequence type 25 carbapenem-resistant hypervirulent Klebsiella pneumoniae from the mid-south region of ChinaJun Li0Zi-Yan Huang1Ting Yu2Xiao-Yan Tao3Yong-Mei Hu4Hai-Chen Wang5Ming-Xiang Zou6Department of Clinical Laboratory, Xiangya Hospital, Central South UniversityDepartment of Clinical Laboratory, Xiangya Hospital, Central South UniversityDepartment of Clinical Laboratory, Xiangya Hospital, Central South UniversityDepartment of Clinical Laboratory, Xiangya Hospital, Central South UniversityDepartment of Clinical Laboratory, Xiangya Hospital, Central South UniversityDepartment of Clinical Laboratory, Xiangya Hospital, Central South UniversityDepartment of Clinical Laboratory, Xiangya Hospital, Central South UniversityAbstract Background The molecular characterization of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) isolates is not well studied. Our goal was to investigate the molecular epidemiology of CR-hvKP strains that were isolated from a Chinese hospital. Results All clinical carbapenem-resistant K. pneumoniae (CR-KP) isolates were collected and identified from patient samples between 2014 and 2017 from a Chinese hospital. The samples were subjected to screening for CR-hvKP by string test and the detection of the aerobactin gene. CR-hvKP isolates were further confirmed through neutrophil phagocytosis and a mice lethality assay. The CR-hvKP isolates were investigated for their capsular genotyping, virulence gene profiles, and the expression of carbapenemase genes by PCR and DNA sequencing. Multilocus sequence type (MLST) and pulsed-field gel electrophoresis (PFGE) were performed to exclude the homology of these isolates. Twenty strains were identified as CR-hvKP. These strains were resistant to imipenem and several other antibiotics, however, most were susceptible to amikacin. Notably, two isolates were not susceptible to tigecycline. Capsular polysaccharide synthesis genotyping revealed that 17 of the 20 CR-hvKP strains belonged to the K2 serotype, while the others belonged to serotypes other than K1, K2, K5, K20, and K57. The strains were found to be positive for 10 types of virulence genes and a variety of these genes coexisted in the same strain. Two carbapenemase genes were identified: bla KPC-2 (13/20) and bla NDM-1 (1/20). PFGE typing revealed eight clusters comprising isolates that belonged to MLST types ST25, ST11 and ST375, respectively. PFGE cluster A was identified as the main cluster, which included 11 isolates that belong to ST25 and mainly from ICU department. Conclusions Our findings suggest that hospital-acquired infections may contribute in part to the CR-hvKP strains identified in this study. It also suggests that ST25 CR-hvKP strain has a clonal distribution in our hospital. Therefore, effective surveillance and strict infection control strategies should be implemented to prevent outbreak by CR-hvKP strains in hospitals setting.http://link.springer.com/article/10.1186/s12866-019-1593-5Carbapenem-resistantCarbapenemaseHypervirulentKlebsiella pneumoniaeSerotype |
| spellingShingle | Jun Li Zi-Yan Huang Ting Yu Xiao-Yan Tao Yong-Mei Hu Hai-Chen Wang Ming-Xiang Zou Isolation and characterization of a sequence type 25 carbapenem-resistant hypervirulent Klebsiella pneumoniae from the mid-south region of China BMC Microbiology Carbapenem-resistant Carbapenemase Hypervirulent Klebsiella pneumoniae Serotype |
| title | Isolation and characterization of a sequence type 25 carbapenem-resistant hypervirulent Klebsiella pneumoniae from the mid-south region of China |
| title_full | Isolation and characterization of a sequence type 25 carbapenem-resistant hypervirulent Klebsiella pneumoniae from the mid-south region of China |
| title_fullStr | Isolation and characterization of a sequence type 25 carbapenem-resistant hypervirulent Klebsiella pneumoniae from the mid-south region of China |
| title_full_unstemmed | Isolation and characterization of a sequence type 25 carbapenem-resistant hypervirulent Klebsiella pneumoniae from the mid-south region of China |
| title_short | Isolation and characterization of a sequence type 25 carbapenem-resistant hypervirulent Klebsiella pneumoniae from the mid-south region of China |
| title_sort | isolation and characterization of a sequence type 25 carbapenem resistant hypervirulent klebsiella pneumoniae from the mid south region of china |
| topic | Carbapenem-resistant Carbapenemase Hypervirulent Klebsiella pneumoniae Serotype |
| url | http://link.springer.com/article/10.1186/s12866-019-1593-5 |
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