Evaluation of immunogenicity of the 2008-2009 seasonal influenza vaccines by microneutralization test
PurposeFor evaluating the immunogenicity of an influenza vaccine, the microneutralization (MN) test has a higher sensitivity and specificity as compared to the hemagglutination inhibition (HI) test. However, the MN test is more time consuming and is difficult to standardize. We performed the MN test...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Pediatric Society
2012-12-01
|
| Series: | Korean Journal of Pediatrics |
| Subjects: | |
| Online Access: | http://kjp.or.kr/upload/pdf/kjped-55-474.pdf |
| _version_ | 1811257200896638976 |
|---|---|
| author | Seung Youn Kim Yun Kyung Kim Byung Wook Eun Nam Hee Kim Eun Kyeong Kang Byong Sop Lee Jung Sub Lim Jun Ah Lee Dong Ho Kim |
| author_facet | Seung Youn Kim Yun Kyung Kim Byung Wook Eun Nam Hee Kim Eun Kyeong Kang Byong Sop Lee Jung Sub Lim Jun Ah Lee Dong Ho Kim |
| author_sort | Seung Youn Kim |
| collection | DOAJ |
| description | PurposeFor evaluating the immunogenicity of an influenza vaccine, the microneutralization (MN) test has a higher sensitivity and specificity as compared to the hemagglutination inhibition (HI) test. However, the MN test is more time consuming and is difficult to standardize. We performed the MN test to determine its usefulness as an alternative or complementary test to the HI test for evaluating the immunogenicity of influenza vaccines.MethodsWe compared the MN test with the HI test using 50 paired samples taken from a previous clinical study (2008-2009) in Korean children under 18 years of age.ResultsThe linear correlation coefficients of the 2 tests for H3N2, H1N1, and influenza B were 0.69, 0.70, and 0.66, respectively. We identified a high index of coincidence between the 2 tests. For an influenza vaccine, the postvaccination seroprotection rates and seroconversion rates determined by the MN test were 78.0% and 96.0%, 90% and 42.0%, and 42.0% and 48.0% for H3N2, H1N1, and influenza B, respectively. Geometric mean titer fold increases of H3N2, H1N1, and influenza B were 2.89, 5.04, and 4.29, respectively, and were 2.5-fold higher. We obtained good results in the evaluation of the immunogenicity of the 2008-2009 seasonal influenza vaccines.ConclusionWe found that the MN test was as effective as the HI test. Therefore, we suggest that the MN test can be used as an alternative or complementary test to the HI test for evaluating the immunogenicity of influenza vaccines. |
| first_indexed | 2024-04-12T17:52:40Z |
| format | Article |
| id | doaj.art-6595652f6f6540e2a5ccb4e7136099e1 |
| institution | Directory Open Access Journal |
| issn | 1738-1061 2092-7258 |
| language | English |
| last_indexed | 2024-04-12T17:52:40Z |
| publishDate | 2012-12-01 |
| publisher | Korean Pediatric Society |
| record_format | Article |
| series | Korean Journal of Pediatrics |
| spelling | doaj.art-6595652f6f6540e2a5ccb4e7136099e12022-12-22T03:22:27ZengKorean Pediatric SocietyKorean Journal of Pediatrics1738-10612092-72582012-12-01551247448010.3345/kjp.2012.55.12.4742012600092Evaluation of immunogenicity of the 2008-2009 seasonal influenza vaccines by microneutralization testSeung Youn Kim0Yun Kyung Kim1Byung Wook Eun2Nam Hee Kim3Eun Kyeong Kang4Byong Sop Lee5Jung Sub Lim6Jun Ah Lee7Dong Ho Kim8Department of Pediatrics, Korean Cancer Center Hospital, Seoul, Korea.Department of Pediatrics, Korea University College of Medicine, Seoul, Korea.Department of Pediatrics, Graduate School of Medicine, Gachon University of Medicine and Science, Incheon, Korea.Department of Pediatrics, Inje University Ilsan Paik Hospital, Inje University College of Medicine, Goyang, Korea.Department of Pediatrics, Dongguk University Ilsan Hospital, Goyang, Korea.Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Korea.Department of Pediatrics, Korean Cancer Center Hospital, Seoul, Korea.Department of Pediatrics, Korean Cancer Center Hospital, Seoul, Korea.Department of Pediatrics, Korean Cancer Center Hospital, Seoul, Korea.PurposeFor evaluating the immunogenicity of an influenza vaccine, the microneutralization (MN) test has a higher sensitivity and specificity as compared to the hemagglutination inhibition (HI) test. However, the MN test is more time consuming and is difficult to standardize. We performed the MN test to determine its usefulness as an alternative or complementary test to the HI test for evaluating the immunogenicity of influenza vaccines.MethodsWe compared the MN test with the HI test using 50 paired samples taken from a previous clinical study (2008-2009) in Korean children under 18 years of age.ResultsThe linear correlation coefficients of the 2 tests for H3N2, H1N1, and influenza B were 0.69, 0.70, and 0.66, respectively. We identified a high index of coincidence between the 2 tests. For an influenza vaccine, the postvaccination seroprotection rates and seroconversion rates determined by the MN test were 78.0% and 96.0%, 90% and 42.0%, and 42.0% and 48.0% for H3N2, H1N1, and influenza B, respectively. Geometric mean titer fold increases of H3N2, H1N1, and influenza B were 2.89, 5.04, and 4.29, respectively, and were 2.5-fold higher. We obtained good results in the evaluation of the immunogenicity of the 2008-2009 seasonal influenza vaccines.ConclusionWe found that the MN test was as effective as the HI test. Therefore, we suggest that the MN test can be used as an alternative or complementary test to the HI test for evaluating the immunogenicity of influenza vaccines.http://kjp.or.kr/upload/pdf/kjped-55-474.pdfNeutralization testsHemagglutination inhibition testInfluenza vaccinesChild |
| spellingShingle | Seung Youn Kim Yun Kyung Kim Byung Wook Eun Nam Hee Kim Eun Kyeong Kang Byong Sop Lee Jung Sub Lim Jun Ah Lee Dong Ho Kim Evaluation of immunogenicity of the 2008-2009 seasonal influenza vaccines by microneutralization test Korean Journal of Pediatrics Neutralization tests Hemagglutination inhibition test Influenza vaccines Child |
| title | Evaluation of immunogenicity of the 2008-2009 seasonal influenza vaccines by microneutralization test |
| title_full | Evaluation of immunogenicity of the 2008-2009 seasonal influenza vaccines by microneutralization test |
| title_fullStr | Evaluation of immunogenicity of the 2008-2009 seasonal influenza vaccines by microneutralization test |
| title_full_unstemmed | Evaluation of immunogenicity of the 2008-2009 seasonal influenza vaccines by microneutralization test |
| title_short | Evaluation of immunogenicity of the 2008-2009 seasonal influenza vaccines by microneutralization test |
| title_sort | evaluation of immunogenicity of the 2008 2009 seasonal influenza vaccines by microneutralization test |
| topic | Neutralization tests Hemagglutination inhibition test Influenza vaccines Child |
| url | http://kjp.or.kr/upload/pdf/kjped-55-474.pdf |
| work_keys_str_mv | AT seungyounkim evaluationofimmunogenicityofthe20082009seasonalinfluenzavaccinesbymicroneutralizationtest AT yunkyungkim evaluationofimmunogenicityofthe20082009seasonalinfluenzavaccinesbymicroneutralizationtest AT byungwookeun evaluationofimmunogenicityofthe20082009seasonalinfluenzavaccinesbymicroneutralizationtest AT namheekim evaluationofimmunogenicityofthe20082009seasonalinfluenzavaccinesbymicroneutralizationtest AT eunkyeongkang evaluationofimmunogenicityofthe20082009seasonalinfluenzavaccinesbymicroneutralizationtest AT byongsoplee evaluationofimmunogenicityofthe20082009seasonalinfluenzavaccinesbymicroneutralizationtest AT jungsublim evaluationofimmunogenicityofthe20082009seasonalinfluenzavaccinesbymicroneutralizationtest AT junahlee evaluationofimmunogenicityofthe20082009seasonalinfluenzavaccinesbymicroneutralizationtest AT donghokim evaluationofimmunogenicityofthe20082009seasonalinfluenzavaccinesbymicroneutralizationtest |