Looking at Biomolecular Interactions through the Lens of Correlated Fluorescence Microscopy and Optical Tweezers
Understanding complex biological events at the molecular level paves the path to determine mechanistic processes across the timescale necessary for breakthrough discoveries. While various conventional biophysical methods provide some information for understanding biological systems, they often lack...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/3/2668 |
| _version_ | 1797624329504030720 |
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| author | Anahita Haghizadeh Mariam Iftikhar Shiba S. Dandpat Trey Simpson |
| author_facet | Anahita Haghizadeh Mariam Iftikhar Shiba S. Dandpat Trey Simpson |
| author_sort | Anahita Haghizadeh |
| collection | DOAJ |
| description | Understanding complex biological events at the molecular level paves the path to determine mechanistic processes across the timescale necessary for breakthrough discoveries. While various conventional biophysical methods provide some information for understanding biological systems, they often lack a complete picture of the molecular-level details of such dynamic processes. Studies at the single-molecule level have emerged to provide crucial missing links to understanding complex and dynamic pathways in biological systems, which are often superseded by bulk biophysical and biochemical studies. Latest developments in techniques combining single-molecule manipulation tools such as optical tweezers and visualization tools such as fluorescence or label-free microscopy have enabled the investigation of complex and dynamic biomolecular interactions at the single-molecule level. In this review, we present recent advances using correlated single-molecule manipulation and visualization-based approaches to obtain a more advanced understanding of the pathways for fundamental biological processes, and how this combination technique is facilitating research in the dynamic single-molecule (DSM), cell biology, and nanomaterials fields. |
| first_indexed | 2024-03-11T09:40:51Z |
| format | Article |
| id | doaj.art-659574bcfddb4048a8a3510d204eb011 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:40:51Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-659574bcfddb4048a8a3510d204eb0112023-11-16T17:00:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01243266810.3390/ijms24032668Looking at Biomolecular Interactions through the Lens of Correlated Fluorescence Microscopy and Optical TweezersAnahita Haghizadeh0Mariam Iftikhar1Shiba S. Dandpat2Trey Simpson3LUMICKS, Waltham, MA 02453, USALUMICKS, Waltham, MA 02453, USALUMICKS, Waltham, MA 02453, USALUMICKS, Waltham, MA 02453, USAUnderstanding complex biological events at the molecular level paves the path to determine mechanistic processes across the timescale necessary for breakthrough discoveries. While various conventional biophysical methods provide some information for understanding biological systems, they often lack a complete picture of the molecular-level details of such dynamic processes. Studies at the single-molecule level have emerged to provide crucial missing links to understanding complex and dynamic pathways in biological systems, which are often superseded by bulk biophysical and biochemical studies. Latest developments in techniques combining single-molecule manipulation tools such as optical tweezers and visualization tools such as fluorescence or label-free microscopy have enabled the investigation of complex and dynamic biomolecular interactions at the single-molecule level. In this review, we present recent advances using correlated single-molecule manipulation and visualization-based approaches to obtain a more advanced understanding of the pathways for fundamental biological processes, and how this combination technique is facilitating research in the dynamic single-molecule (DSM), cell biology, and nanomaterials fields.https://www.mdpi.com/1422-0067/24/3/2668single-molecule biologybiophysicsoptical tweezersfluorescence microscopycorrelated force-fluorescence |
| spellingShingle | Anahita Haghizadeh Mariam Iftikhar Shiba S. Dandpat Trey Simpson Looking at Biomolecular Interactions through the Lens of Correlated Fluorescence Microscopy and Optical Tweezers International Journal of Molecular Sciences single-molecule biology biophysics optical tweezers fluorescence microscopy correlated force-fluorescence |
| title | Looking at Biomolecular Interactions through the Lens of Correlated Fluorescence Microscopy and Optical Tweezers |
| title_full | Looking at Biomolecular Interactions through the Lens of Correlated Fluorescence Microscopy and Optical Tweezers |
| title_fullStr | Looking at Biomolecular Interactions through the Lens of Correlated Fluorescence Microscopy and Optical Tweezers |
| title_full_unstemmed | Looking at Biomolecular Interactions through the Lens of Correlated Fluorescence Microscopy and Optical Tweezers |
| title_short | Looking at Biomolecular Interactions through the Lens of Correlated Fluorescence Microscopy and Optical Tweezers |
| title_sort | looking at biomolecular interactions through the lens of correlated fluorescence microscopy and optical tweezers |
| topic | single-molecule biology biophysics optical tweezers fluorescence microscopy correlated force-fluorescence |
| url | https://www.mdpi.com/1422-0067/24/3/2668 |
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