Role of subtyping in detecting <it>Salmonella </it>cross contamination in the laboratory
<p>Abstract</p> <p>Background</p> <p>With the exception of <it>M. tuberculosis</it>, little has been published on the problems of cross-contamination in bacteriology laboratories. We performed a retrospective analysis of subtyping data from the National <...
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| Format: | Article |
| Language: | English |
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BMC
2009-07-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/9/155 |
| _version_ | 1828746171809529856 |
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| author | Devane Genevieve Doran Geraldine Connor Jean O De Lappe Niall Cormican Martin |
| author_facet | Devane Genevieve Doran Geraldine Connor Jean O De Lappe Niall Cormican Martin |
| author_sort | Devane Genevieve |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>With the exception of <it>M. tuberculosis</it>, little has been published on the problems of cross-contamination in bacteriology laboratories. We performed a retrospective analysis of subtyping data from the National <it>Salmonella </it>Reference Laboratory (Ireland) from 2000–2007 to identify likely incidents of laboratory cross contamination.</p> <p>Methods</p> <p>Serotyping and antimicrobial susceptibility testing was performed on all <it>Salmonella </it>isolates received in the NSRL. Phage typing was performed on all <it>S</it>. Typhimurium and <it>S</it>. Enteritidis isolates while multi-locus variance analysis (MLVA) was performed on selected <it>S</it>. Typhimurium isolates. Pulsed field gel electrophoresis (PFGE) using the PulseNet standard protocol was performed on selected isolates of various serovars.</p> <p>Results</p> <p>Twenty-three incidents involving fifty-six isolates were identified as likely to represent cross contamination. The probable sources of contamination identified were the laboratory positive control isolate (n = 13), other test isolates (n = 9) or proficiency test samples (n = 1).</p> <p>Conclusion</p> <p>The scale of laboratory cross-contamination in bacteriology is most likely under recognized. Testing laboratories should be aware of the potential for cross-contamination, regularly review protocols to minimize its occurrence and consider it as a possibility when unexpected results are obtained.</p> |
| first_indexed | 2024-04-14T04:18:30Z |
| format | Article |
| id | doaj.art-659c36ac34bc43d29dee1148398ed99b |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-14T04:18:30Z |
| publishDate | 2009-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-659c36ac34bc43d29dee1148398ed99b2022-12-22T02:12:41ZengBMCBMC Microbiology1471-21802009-07-019115510.1186/1471-2180-9-155Role of subtyping in detecting <it>Salmonella </it>cross contamination in the laboratoryDevane GenevieveDoran GeraldineConnor Jean ODe Lappe NiallCormican Martin<p>Abstract</p> <p>Background</p> <p>With the exception of <it>M. tuberculosis</it>, little has been published on the problems of cross-contamination in bacteriology laboratories. We performed a retrospective analysis of subtyping data from the National <it>Salmonella </it>Reference Laboratory (Ireland) from 2000–2007 to identify likely incidents of laboratory cross contamination.</p> <p>Methods</p> <p>Serotyping and antimicrobial susceptibility testing was performed on all <it>Salmonella </it>isolates received in the NSRL. Phage typing was performed on all <it>S</it>. Typhimurium and <it>S</it>. Enteritidis isolates while multi-locus variance analysis (MLVA) was performed on selected <it>S</it>. Typhimurium isolates. Pulsed field gel electrophoresis (PFGE) using the PulseNet standard protocol was performed on selected isolates of various serovars.</p> <p>Results</p> <p>Twenty-three incidents involving fifty-six isolates were identified as likely to represent cross contamination. The probable sources of contamination identified were the laboratory positive control isolate (n = 13), other test isolates (n = 9) or proficiency test samples (n = 1).</p> <p>Conclusion</p> <p>The scale of laboratory cross-contamination in bacteriology is most likely under recognized. Testing laboratories should be aware of the potential for cross-contamination, regularly review protocols to minimize its occurrence and consider it as a possibility when unexpected results are obtained.</p>http://www.biomedcentral.com/1471-2180/9/155 |
| spellingShingle | Devane Genevieve Doran Geraldine Connor Jean O De Lappe Niall Cormican Martin Role of subtyping in detecting <it>Salmonella </it>cross contamination in the laboratory BMC Microbiology |
| title | Role of subtyping in detecting <it>Salmonella </it>cross contamination in the laboratory |
| title_full | Role of subtyping in detecting <it>Salmonella </it>cross contamination in the laboratory |
| title_fullStr | Role of subtyping in detecting <it>Salmonella </it>cross contamination in the laboratory |
| title_full_unstemmed | Role of subtyping in detecting <it>Salmonella </it>cross contamination in the laboratory |
| title_short | Role of subtyping in detecting <it>Salmonella </it>cross contamination in the laboratory |
| title_sort | role of subtyping in detecting it salmonella it cross contamination in the laboratory |
| url | http://www.biomedcentral.com/1471-2180/9/155 |
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