Genome-Wide Identification of Histone Modifiers and Their Expression Patterns during Fruit Abscission in Litchi
Modifications to histones, including acetylation and methylation processes, play crucial roles in the regulation of gene expression in plant development as well as in stress responses. However, limited information on the enzymes catalyzing histone acetylation and methylation in non-model plants is c...
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| Format: | Article |
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Frontiers Media S.A.
2017-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.00639/full |
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| author | Jianguo Li Jianguo Li Minglei Zhao Minglei Zhao Manjun Peng Manjun Peng Peiyuan Ying Peiyuan Ying Xuncheng Liu Caiqin Li Caiqin Li Rui Xia Rui Xia |
| author_facet | Jianguo Li Jianguo Li Minglei Zhao Minglei Zhao Manjun Peng Manjun Peng Peiyuan Ying Peiyuan Ying Xuncheng Liu Caiqin Li Caiqin Li Rui Xia Rui Xia |
| author_sort | Jianguo Li |
| collection | DOAJ |
| description | Modifications to histones, including acetylation and methylation processes, play crucial roles in the regulation of gene expression in plant development as well as in stress responses. However, limited information on the enzymes catalyzing histone acetylation and methylation in non-model plants is currently available. In this study, several histone modifier (HM) types, including six histone acetyltransferases (HATs), 11 histone deacetylases (HDACs), 48 histone methyltransferases (HMTs), and 22 histone demethylases (HDMs), are identified in litchi (Litchi chinensis Sonn. cv. Feizixiao) based on similarities in their sequences to homologs in Arabidopsis (A. thaliana), tomato (Solanum lycopersicum), and rice (Oryza sativa). Phylogenetic analyses reveal that HM enzymes can be grouped into four HAT, two HDAC, two HMT, and two HDM subfamilies, respectively, while further expression profile analyses demonstrate that 17 HMs were significantly altered during fruit abscission in two field treatments. Analyses reveal that these genes exhibit four distinct patterns of expression in response to fruit abscission, while an in vitro assay was used to confirm the HDAC activity of LcHDA2, LcHDA6, and LcSRT2. Our findings are the first in-depth analysis of HMs in the litchi genome, and imply that some are likely to play important roles in fruit abscission in this commercially important plant. |
| first_indexed | 2024-12-11T09:26:13Z |
| format | Article |
| id | doaj.art-659eafa473d04398812cd686cd03bde8 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T09:26:13Z |
| publishDate | 2017-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-659eafa473d04398812cd686cd03bde82022-12-22T01:13:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-04-01810.3389/fpls.2017.00639221572Genome-Wide Identification of Histone Modifiers and Their Expression Patterns during Fruit Abscission in LitchiJianguo Li0Jianguo Li1Minglei Zhao2Minglei Zhao3Manjun Peng4Manjun Peng5Peiyuan Ying6Peiyuan Ying7Xuncheng Liu8Caiqin Li9Caiqin Li10Rui Xia11Rui Xia12State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, China Litchi Research Center, South China Agricultural UniversityGuangzhou, ChinaGuangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural UniversityGuangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, China Litchi Research Center, South China Agricultural UniversityGuangzhou, ChinaGuangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural UniversityGuangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, China Litchi Research Center, South China Agricultural UniversityGuangzhou, ChinaGuangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural UniversityGuangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, China Litchi Research Center, South China Agricultural UniversityGuangzhou, ChinaGuangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural UniversityGuangzhou, ChinaKey Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of SciencesGuangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, China Litchi Research Center, South China Agricultural UniversityGuangzhou, ChinaGuangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural UniversityGuangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, China Litchi Research Center, South China Agricultural UniversityGuangzhou, ChinaGuangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural UniversityGuangzhou, ChinaModifications to histones, including acetylation and methylation processes, play crucial roles in the regulation of gene expression in plant development as well as in stress responses. However, limited information on the enzymes catalyzing histone acetylation and methylation in non-model plants is currently available. In this study, several histone modifier (HM) types, including six histone acetyltransferases (HATs), 11 histone deacetylases (HDACs), 48 histone methyltransferases (HMTs), and 22 histone demethylases (HDMs), are identified in litchi (Litchi chinensis Sonn. cv. Feizixiao) based on similarities in their sequences to homologs in Arabidopsis (A. thaliana), tomato (Solanum lycopersicum), and rice (Oryza sativa). Phylogenetic analyses reveal that HM enzymes can be grouped into four HAT, two HDAC, two HMT, and two HDM subfamilies, respectively, while further expression profile analyses demonstrate that 17 HMs were significantly altered during fruit abscission in two field treatments. Analyses reveal that these genes exhibit four distinct patterns of expression in response to fruit abscission, while an in vitro assay was used to confirm the HDAC activity of LcHDA2, LcHDA6, and LcSRT2. Our findings are the first in-depth analysis of HMs in the litchi genome, and imply that some are likely to play important roles in fruit abscission in this commercially important plant.http://journal.frontiersin.org/article/10.3389/fpls.2017.00639/fulllitchifruit abscissionhistone modifiersgene expressionHDAC activity |
| spellingShingle | Jianguo Li Jianguo Li Minglei Zhao Minglei Zhao Manjun Peng Manjun Peng Peiyuan Ying Peiyuan Ying Xuncheng Liu Caiqin Li Caiqin Li Rui Xia Rui Xia Genome-Wide Identification of Histone Modifiers and Their Expression Patterns during Fruit Abscission in Litchi Frontiers in Plant Science litchi fruit abscission histone modifiers gene expression HDAC activity |
| title | Genome-Wide Identification of Histone Modifiers and Their Expression Patterns during Fruit Abscission in Litchi |
| title_full | Genome-Wide Identification of Histone Modifiers and Their Expression Patterns during Fruit Abscission in Litchi |
| title_fullStr | Genome-Wide Identification of Histone Modifiers and Their Expression Patterns during Fruit Abscission in Litchi |
| title_full_unstemmed | Genome-Wide Identification of Histone Modifiers and Their Expression Patterns during Fruit Abscission in Litchi |
| title_short | Genome-Wide Identification of Histone Modifiers and Their Expression Patterns during Fruit Abscission in Litchi |
| title_sort | genome wide identification of histone modifiers and their expression patterns during fruit abscission in litchi |
| topic | litchi fruit abscission histone modifiers gene expression HDAC activity |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.00639/full |
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