Screening of Genes Related to Fat Deposition of Pekin Ducks Based on Transcriptome Analysis
Subcutaneous fat deposition is an important index with which to evaluate meat-producing ducks, and affects their meat quality and feed conversion rate. Studying the differentially expressed genes in subcutaneous fat will help to comprehensively understand the potential mechanisms regulating fat depo...
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MDPI AG
2024-01-01
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| 丛编: | Animals |
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| 在线阅读: | https://www.mdpi.com/2076-2615/14/2/268 |
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| author | Bozhi Shi Ziyue Zhang Xueze Lv Keying An Lei Li Zhaofei Xia |
| author_facet | Bozhi Shi Ziyue Zhang Xueze Lv Keying An Lei Li Zhaofei Xia |
| author_sort | Bozhi Shi |
| collection | DOAJ |
| description | Subcutaneous fat deposition is an important index with which to evaluate meat-producing ducks, and affects their meat quality and feed conversion rate. Studying the differentially expressed genes in subcutaneous fat will help to comprehensively understand the potential mechanisms regulating fat deposition in ducks. In this study, 72 Nankou 1 Pekin Ducks and 72 Jingdian Pekin Ducks (half male and half female) at 42 days of age were selected for slaughter performance and transcriptome analysis. The results showed that the breast-muscle yield of Nankou 1 ducks was significantly higher than that of Jingdian ducks, but that the abdominal fat yield and subcutaneous fat yield were higher than that of Jingdian ducks. Thousands of DEGs, including many important genes involved in fat metabolism regulation, were detected by transcriptome. KEGG enrichment analysis showed that the DEGs were significantly enriched on pathways such as regulation of lipolysis in adipocytes, primary bile acid biosynthesis, and biosynthesis of unsaturated fatty acids. SCD, FGF7, LTBP1, PNPLA3, ADCY2, and ACOT8 were selected as candidate genes for regulating subcutaneous fat deposition. The results indicated that Nankou 1 had superior fat deposition ability compared to Jingdian ducks, and that the candidate genes regulated fat deposition by regulating fat synthesis and decomposition. |
| first_indexed | 2024-03-08T11:07:15Z |
| format | Article |
| id | doaj.art-65a0c15efc4e4b81b5a3dc959cdd0876 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-08T11:07:15Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-65a0c15efc4e4b81b5a3dc959cdd08762024-01-26T14:32:32ZengMDPI AGAnimals2076-26152024-01-0114226810.3390/ani14020268Screening of Genes Related to Fat Deposition of Pekin Ducks Based on Transcriptome AnalysisBozhi Shi0Ziyue Zhang1Xueze Lv2Keying An3Lei Li4Zhaofei Xia5College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaCollege of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaBeijing General Station of Animal Husbandry, Beijing 100107, ChinaCollege of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaCollege of Veterinary Medicine, Yunnan Agricultural University, Kunming 650500, ChinaCollege of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaSubcutaneous fat deposition is an important index with which to evaluate meat-producing ducks, and affects their meat quality and feed conversion rate. Studying the differentially expressed genes in subcutaneous fat will help to comprehensively understand the potential mechanisms regulating fat deposition in ducks. In this study, 72 Nankou 1 Pekin Ducks and 72 Jingdian Pekin Ducks (half male and half female) at 42 days of age were selected for slaughter performance and transcriptome analysis. The results showed that the breast-muscle yield of Nankou 1 ducks was significantly higher than that of Jingdian ducks, but that the abdominal fat yield and subcutaneous fat yield were higher than that of Jingdian ducks. Thousands of DEGs, including many important genes involved in fat metabolism regulation, were detected by transcriptome. KEGG enrichment analysis showed that the DEGs were significantly enriched on pathways such as regulation of lipolysis in adipocytes, primary bile acid biosynthesis, and biosynthesis of unsaturated fatty acids. SCD, FGF7, LTBP1, PNPLA3, ADCY2, and ACOT8 were selected as candidate genes for regulating subcutaneous fat deposition. The results indicated that Nankou 1 had superior fat deposition ability compared to Jingdian ducks, and that the candidate genes regulated fat deposition by regulating fat synthesis and decomposition.https://www.mdpi.com/2076-2615/14/2/268Pekin ducksubcutaneous fatslaughter performanceRNA-seqfat metabolism |
| spellingShingle | Bozhi Shi Ziyue Zhang Xueze Lv Keying An Lei Li Zhaofei Xia Screening of Genes Related to Fat Deposition of Pekin Ducks Based on Transcriptome Analysis Animals Pekin duck subcutaneous fat slaughter performance RNA-seq fat metabolism |
| title | Screening of Genes Related to Fat Deposition of Pekin Ducks Based on Transcriptome Analysis |
| title_full | Screening of Genes Related to Fat Deposition of Pekin Ducks Based on Transcriptome Analysis |
| title_fullStr | Screening of Genes Related to Fat Deposition of Pekin Ducks Based on Transcriptome Analysis |
| title_full_unstemmed | Screening of Genes Related to Fat Deposition of Pekin Ducks Based on Transcriptome Analysis |
| title_short | Screening of Genes Related to Fat Deposition of Pekin Ducks Based on Transcriptome Analysis |
| title_sort | screening of genes related to fat deposition of pekin ducks based on transcriptome analysis |
| topic | Pekin duck subcutaneous fat slaughter performance RNA-seq fat metabolism |
| url | https://www.mdpi.com/2076-2615/14/2/268 |
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