Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay
A colorimetric loop-mediated isothermal amplification assay detects changes in pH during amplification based on color changes at a constant temperature. Currently, various studies have focused on developing and assessing molecular point-of-care testing instruments. In this study, we evaluated amplif...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Future Science Ltd
2023-12-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2023-0104 |
| _version_ | 1797382441734766592 |
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| author | Seongsoo Hong Jeongho Park Jaekyung Bok Euna Cho Joowon Rhee |
| author_facet | Seongsoo Hong Jeongho Park Jaekyung Bok Euna Cho Joowon Rhee |
| author_sort | Seongsoo Hong |
| collection | DOAJ |
| description | A colorimetric loop-mediated isothermal amplification assay detects changes in pH during amplification based on color changes at a constant temperature. Currently, various studies have focused on developing and assessing molecular point-of-care testing instruments. In this study, we evaluated amplified DNA concentrations measured using the colorimetric LAMP assay of the 1POT™ Professional device (1drop Inc, Korea). Results of the 1POT analysis of clinical samples were compared with measurements obtained from the Qubit™ 4 and NanoDrop™ 2000 devices (both from Thermo Fisher Scientific, MA, USA). These results showed a correlation of 0.98 (95% CI: 0.96–0.99) and 0.96 (95% CI: 0.92–0.98) between 1POT and the Qubit and NanoDrop. 1POT can measure amplified DNA accurately and is suitable for on-site molecular diagnostics. |
| first_indexed | 2024-03-08T21:06:26Z |
| format | Article |
| id | doaj.art-65a6517f55a649b3a016deeb3582a1b4 |
| institution | Directory Open Access Journal |
| issn | 0736-6205 1940-9818 |
| language | English |
| last_indexed | 2024-03-08T21:06:26Z |
| publishDate | 2023-12-01 |
| publisher | Future Science Ltd |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj.art-65a6517f55a649b3a016deeb3582a1b42023-12-22T12:35:04ZengFuture Science LtdBioTechniques0736-62051940-98182023-12-0110.2144/btn-2023-0104Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assaySeongsoo Hong0Jeongho Park1Jaekyung Bok2Euna Cho3Joowon Rhee411drop Inc, 215, Galmachi-ro, Jungwon-gu, Seongnam-si, Gyeonggi-do, Korea11drop Inc, 215, Galmachi-ro, Jungwon-gu, Seongnam-si, Gyeonggi-do, Korea11drop Inc, 215, Galmachi-ro, Jungwon-gu, Seongnam-si, Gyeonggi-do, Korea11drop Inc, 215, Galmachi-ro, Jungwon-gu, Seongnam-si, Gyeonggi-do, Korea11drop Inc, 215, Galmachi-ro, Jungwon-gu, Seongnam-si, Gyeonggi-do, KoreaA colorimetric loop-mediated isothermal amplification assay detects changes in pH during amplification based on color changes at a constant temperature. Currently, various studies have focused on developing and assessing molecular point-of-care testing instruments. In this study, we evaluated amplified DNA concentrations measured using the colorimetric LAMP assay of the 1POT™ Professional device (1drop Inc, Korea). Results of the 1POT analysis of clinical samples were compared with measurements obtained from the Qubit™ 4 and NanoDrop™ 2000 devices (both from Thermo Fisher Scientific, MA, USA). These results showed a correlation of 0.98 (95% CI: 0.96–0.99) and 0.96 (95% CI: 0.92–0.98) between 1POT and the Qubit and NanoDrop. 1POT can measure amplified DNA accurately and is suitable for on-site molecular diagnostics.https://www.future-science.com/doi/10.2144/btn-2023-01041POT™loop-mediated isothermal amplificationNanoDrop™point-of-care testing deviceQubit™ |
| spellingShingle | Seongsoo Hong Jeongho Park Jaekyung Bok Euna Cho Joowon Rhee Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay BioTechniques 1POT™ loop-mediated isothermal amplification NanoDrop™ point-of-care testing device Qubit™ |
| title | Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay |
| title_full | Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay |
| title_fullStr | Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay |
| title_full_unstemmed | Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay |
| title_short | Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay |
| title_sort | assessment of measurement accuracy of amplified dna using a colorimetric loop mediated isothermal amplification assay |
| topic | 1POT™ loop-mediated isothermal amplification NanoDrop™ point-of-care testing device Qubit™ |
| url | https://www.future-science.com/doi/10.2144/btn-2023-0104 |
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