Incidence of dengue illness in Mexican people aged 6 months to 50 years old: A prospective cohort study conducted in Jalisco.
<h4>Background and objectives</h4>The burden of dengue virus (DENV), a mosquito-borne pathogen, remains difficult to assess due to misdiagnosis and underreporting. Moreover, the large proportion of asymptomatic dengue cases impairs comprehensive assessment of its epidemiology even where...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0250253 |
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| author | Rodrigo DeAntonio Gerardo Amaya-Tapia Gabriela Ibarra-Nieto Gloria Huerta Silvia Damaso Adrienne Guignard Melanie de Boer |
| author_facet | Rodrigo DeAntonio Gerardo Amaya-Tapia Gabriela Ibarra-Nieto Gloria Huerta Silvia Damaso Adrienne Guignard Melanie de Boer |
| author_sort | Rodrigo DeAntonio |
| collection | DOAJ |
| description | <h4>Background and objectives</h4>The burden of dengue virus (DENV), a mosquito-borne pathogen, remains difficult to assess due to misdiagnosis and underreporting. Moreover, the large proportion of asymptomatic dengue cases impairs comprehensive assessment of its epidemiology even where effective surveillance systems are in place. We conducted a prospective community-based study to assess the incidence of symptomatic dengue cases in Zapopan and neighboring municipalities in the state of Jalisco, Mexico.<h4>Methods</h4>Healthy subjects aged 6 months to 50 years living in households located in the Zapopan and neighboring municipalities were enrolled for a 24-month follow-up study (NCT02766088). Serostatus was determined at enrolment and weekly contacts were conducted via phone calls and home visits. Participants had to report any febrile episode lasting for at least two days. Suspected dengue cases were tested by reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR), detection of non-structural protein 1 (NS1), anti-DENV immunoglobulin G and M (IgG and IgM) assays.<h4>Results</h4>A total of 350 individuals from 87 households were enrolled. The overall seroprevalence of anti-DENV IgG at enrolment was 19.4% (95% confidence interval [CI] 14.5-25.6) with the highest seroprevalence rate observed in the adult group. Over the 27-month study period from July 2016 to September 2018, a total of 18 suspected dengue cases were reported. Four cases were confirmed by RT-qPCR and serotyped as DENV-1. A fifth case was confirmed by the NS1 assay. The 13 remaining suspected cases were tested negative by these assays. Based on the 5 virologically confirmed cases, symptomatic dengue incidence proportion of 1.4% (95%CI 0.5-3.8) was estimated. No severe cases or hospitalizations occurred during the study.<h4>Conclusion</h4>Community-based active surveillance was shown as efficient to detect symptomatic dengue cases.<h4>Clinical trial registration</h4>NCT02766088. |
| first_indexed | 2024-12-21T07:43:02Z |
| format | Article |
| id | doaj.art-65abee4852534812927fbbaeaac04740 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T07:43:02Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-65abee4852534812927fbbaeaac047402022-12-21T19:11:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01165e025025310.1371/journal.pone.0250253Incidence of dengue illness in Mexican people aged 6 months to 50 years old: A prospective cohort study conducted in Jalisco.Rodrigo DeAntonioGerardo Amaya-TapiaGabriela Ibarra-NietoGloria HuertaSilvia DamasoAdrienne GuignardMelanie de Boer<h4>Background and objectives</h4>The burden of dengue virus (DENV), a mosquito-borne pathogen, remains difficult to assess due to misdiagnosis and underreporting. Moreover, the large proportion of asymptomatic dengue cases impairs comprehensive assessment of its epidemiology even where effective surveillance systems are in place. We conducted a prospective community-based study to assess the incidence of symptomatic dengue cases in Zapopan and neighboring municipalities in the state of Jalisco, Mexico.<h4>Methods</h4>Healthy subjects aged 6 months to 50 years living in households located in the Zapopan and neighboring municipalities were enrolled for a 24-month follow-up study (NCT02766088). Serostatus was determined at enrolment and weekly contacts were conducted via phone calls and home visits. Participants had to report any febrile episode lasting for at least two days. Suspected dengue cases were tested by reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR), detection of non-structural protein 1 (NS1), anti-DENV immunoglobulin G and M (IgG and IgM) assays.<h4>Results</h4>A total of 350 individuals from 87 households were enrolled. The overall seroprevalence of anti-DENV IgG at enrolment was 19.4% (95% confidence interval [CI] 14.5-25.6) with the highest seroprevalence rate observed in the adult group. Over the 27-month study period from July 2016 to September 2018, a total of 18 suspected dengue cases were reported. Four cases were confirmed by RT-qPCR and serotyped as DENV-1. A fifth case was confirmed by the NS1 assay. The 13 remaining suspected cases were tested negative by these assays. Based on the 5 virologically confirmed cases, symptomatic dengue incidence proportion of 1.4% (95%CI 0.5-3.8) was estimated. No severe cases or hospitalizations occurred during the study.<h4>Conclusion</h4>Community-based active surveillance was shown as efficient to detect symptomatic dengue cases.<h4>Clinical trial registration</h4>NCT02766088.https://doi.org/10.1371/journal.pone.0250253 |
| spellingShingle | Rodrigo DeAntonio Gerardo Amaya-Tapia Gabriela Ibarra-Nieto Gloria Huerta Silvia Damaso Adrienne Guignard Melanie de Boer Incidence of dengue illness in Mexican people aged 6 months to 50 years old: A prospective cohort study conducted in Jalisco. PLoS ONE |
| title | Incidence of dengue illness in Mexican people aged 6 months to 50 years old: A prospective cohort study conducted in Jalisco. |
| title_full | Incidence of dengue illness in Mexican people aged 6 months to 50 years old: A prospective cohort study conducted in Jalisco. |
| title_fullStr | Incidence of dengue illness in Mexican people aged 6 months to 50 years old: A prospective cohort study conducted in Jalisco. |
| title_full_unstemmed | Incidence of dengue illness in Mexican people aged 6 months to 50 years old: A prospective cohort study conducted in Jalisco. |
| title_short | Incidence of dengue illness in Mexican people aged 6 months to 50 years old: A prospective cohort study conducted in Jalisco. |
| title_sort | incidence of dengue illness in mexican people aged 6 months to 50 years old a prospective cohort study conducted in jalisco |
| url | https://doi.org/10.1371/journal.pone.0250253 |
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