Anti-Inflammatory Property Establishment of Fulvic Acid Transdermal Patch in Animal Model
The formulation of the transdermal patch with fulvic acid (FA) on an emulsion basis using pluronic Kolliphor<sup>®</sup> p237 as a permeability enhancer was developed and studied for anti-inflammatory properties. FA was isolated from the peat in the Nizhny Novgorod region of Russia and c...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Scientia Pharmaceutica |
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| Online Access: | https://www.mdpi.com/2218-0532/91/4/45 |
| _version_ | 1797379423954010112 |
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| author | Maria A. Konnova Alexander A. Volkov Anna G. Solovyeva Peter V. Peretyagin Nina B. Melnikova |
| author_facet | Maria A. Konnova Alexander A. Volkov Anna G. Solovyeva Peter V. Peretyagin Nina B. Melnikova |
| author_sort | Maria A. Konnova |
| collection | DOAJ |
| description | The formulation of the transdermal patch with fulvic acid (FA) on an emulsion basis using pluronic Kolliphor<sup>®</sup> p237 as a permeability enhancer was developed and studied for anti-inflammatory properties. FA was isolated from the peat in the Nizhny Novgorod region of Russia and characterized as a potential active pharmaceutical ingredient. In vitro studies of the release of FA from the transdermal patch, as well as the FA penetration through the acetyl cellulose membrane using the Franz diffusion cell, showed its high efficiency (56% and 90%, respectively, in 8 h). In the in vivo experiment, qualitative and quantitative features of the rat knee caused by complete Freund’s adjuvant-induced arthritis (morphological changes, the FA influence on the biochemical indexes) were studied. The inflammatory process that developed within 15 days was accompanied by the activation of antioxidant oxidoreductase enzymes (by 50–70%), the increase in the cross-sectional diameter of the cartilage, and the increase in the values of marker indicators of the process of rheumatoid arthritis. Within 7 days of treatment, under the influence of FA, the values of ESR, RF, leukocytes, C-reactive protein, as well as the biochemical parameters characterizing oxidative stress (SOD, catalase, glutathione reductase, LDH, G6PD) normalized, and the edema reduced. These results may be useful for arthritis treatment using the transdermal patch with FA. |
| first_indexed | 2024-03-08T20:23:02Z |
| format | Article |
| id | doaj.art-65ae1e8a61a741a6a46317d8d5222c85 |
| institution | Directory Open Access Journal |
| issn | 0036-8709 2218-0532 |
| language | English |
| last_indexed | 2024-03-08T20:23:02Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Scientia Pharmaceutica |
| spelling | doaj.art-65ae1e8a61a741a6a46317d8d5222c852023-12-22T14:39:52ZengMDPI AGScientia Pharmaceutica0036-87092218-05322023-09-019144510.3390/scipharm91040045Anti-Inflammatory Property Establishment of Fulvic Acid Transdermal Patch in Animal ModelMaria A. Konnova0Alexander A. Volkov1Anna G. Solovyeva2Peter V. Peretyagin3Nina B. Melnikova4Department of Pharmaceutical Chemistry, Privolzhsky Research Medical University, 10/1 Minin Sq., 603950 Nizhny Novgorod, RussiaDepartment of Pharmaceutical Chemistry, Privolzhsky Research Medical University, 10/1 Minin Sq., 603950 Nizhny Novgorod, RussiaDepartment of Experimental Medicine, Privolzhsky Research Medical University, 10/1 Minin Sq., 603950 Nizhny Novgorod, RussiaDepartment of Experimental Medicine, Privolzhsky Research Medical University, 10/1 Minin Sq., 603950 Nizhny Novgorod, RussiaFaculty of Chemistry, Lobachevsky State University, 23 Gagarin Av., 603022 Nizhny Novgorod, RussiaThe formulation of the transdermal patch with fulvic acid (FA) on an emulsion basis using pluronic Kolliphor<sup>®</sup> p237 as a permeability enhancer was developed and studied for anti-inflammatory properties. FA was isolated from the peat in the Nizhny Novgorod region of Russia and characterized as a potential active pharmaceutical ingredient. In vitro studies of the release of FA from the transdermal patch, as well as the FA penetration through the acetyl cellulose membrane using the Franz diffusion cell, showed its high efficiency (56% and 90%, respectively, in 8 h). In the in vivo experiment, qualitative and quantitative features of the rat knee caused by complete Freund’s adjuvant-induced arthritis (morphological changes, the FA influence on the biochemical indexes) were studied. The inflammatory process that developed within 15 days was accompanied by the activation of antioxidant oxidoreductase enzymes (by 50–70%), the increase in the cross-sectional diameter of the cartilage, and the increase in the values of marker indicators of the process of rheumatoid arthritis. Within 7 days of treatment, under the influence of FA, the values of ESR, RF, leukocytes, C-reactive protein, as well as the biochemical parameters characterizing oxidative stress (SOD, catalase, glutathione reductase, LDH, G6PD) normalized, and the edema reduced. These results may be useful for arthritis treatment using the transdermal patch with FA.https://www.mdpi.com/2218-0532/91/4/45fulvic acidhumic substancestransdermal patchrelease and permeabilitycomplete Freund’s adjuvant-induced arthritisoxidoreductase |
| spellingShingle | Maria A. Konnova Alexander A. Volkov Anna G. Solovyeva Peter V. Peretyagin Nina B. Melnikova Anti-Inflammatory Property Establishment of Fulvic Acid Transdermal Patch in Animal Model Scientia Pharmaceutica fulvic acid humic substances transdermal patch release and permeability complete Freund’s adjuvant-induced arthritis oxidoreductase |
| title | Anti-Inflammatory Property Establishment of Fulvic Acid Transdermal Patch in Animal Model |
| title_full | Anti-Inflammatory Property Establishment of Fulvic Acid Transdermal Patch in Animal Model |
| title_fullStr | Anti-Inflammatory Property Establishment of Fulvic Acid Transdermal Patch in Animal Model |
| title_full_unstemmed | Anti-Inflammatory Property Establishment of Fulvic Acid Transdermal Patch in Animal Model |
| title_short | Anti-Inflammatory Property Establishment of Fulvic Acid Transdermal Patch in Animal Model |
| title_sort | anti inflammatory property establishment of fulvic acid transdermal patch in animal model |
| topic | fulvic acid humic substances transdermal patch release and permeability complete Freund’s adjuvant-induced arthritis oxidoreductase |
| url | https://www.mdpi.com/2218-0532/91/4/45 |
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