Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes
PAM requirement is a constraint for genome editing but this has been circumvented by engineered Cas9 nucleases as SpG and SpRY recognizing minimal PAM sequences. Here, the authors validate and optimize SpG and SpRY in vivo expanding the targeting landscape in animals.
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-05-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-30228-4 |
| _version_ | 1818201353735897088 |
|---|---|
| author | Jeremy Vicencio Carlos Sánchez-Bolaños Ismael Moreno-Sánchez David Brena Charles E. Vejnar Dmytro Kukhtar Miguel Ruiz-López Mariona Cots-Ponjoan Alejandro Rubio Natalia Rodrigo Melero Jesús Crespo-Cuadrado Carlo Carolis Antonio J. Pérez-Pulido Antonio J. Giráldez Benjamin P. Kleinstiver Julián Cerón Miguel A. Moreno-Mateos |
| author_facet | Jeremy Vicencio Carlos Sánchez-Bolaños Ismael Moreno-Sánchez David Brena Charles E. Vejnar Dmytro Kukhtar Miguel Ruiz-López Mariona Cots-Ponjoan Alejandro Rubio Natalia Rodrigo Melero Jesús Crespo-Cuadrado Carlo Carolis Antonio J. Pérez-Pulido Antonio J. Giráldez Benjamin P. Kleinstiver Julián Cerón Miguel A. Moreno-Mateos |
| author_sort | Jeremy Vicencio |
| collection | DOAJ |
| description | PAM requirement is a constraint for genome editing but this has been circumvented by engineered Cas9 nucleases as SpG and SpRY recognizing minimal PAM sequences. Here, the authors validate and optimize SpG and SpRY in vivo expanding the targeting landscape in animals. |
| first_indexed | 2024-12-12T02:52:13Z |
| format | Article |
| id | doaj.art-65af9233becc4657b1eecfc5e6d8262e |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-12T02:52:13Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-65af9233becc4657b1eecfc5e6d8262e2022-12-22T00:40:52ZengNature PortfolioNature Communications2041-17232022-05-0113111310.1038/s41467-022-30228-4Genome editing in animals with minimal PAM CRISPR-Cas9 enzymesJeremy Vicencio0Carlos Sánchez-Bolaños1Ismael Moreno-Sánchez2David Brena3Charles E. Vejnar4Dmytro Kukhtar5Miguel Ruiz-López6Mariona Cots-Ponjoan7Alejandro Rubio8Natalia Rodrigo Melero9Jesús Crespo-Cuadrado10Carlo Carolis11Antonio J. Pérez-Pulido12Antonio J. Giráldez13Benjamin P. Kleinstiver14Julián Cerón15Miguel A. Moreno-Mateos16Modeling human diseases in C. elegans Group; Genes, Disease and Therapy Program, Institut d’Investigació Biomèdica de Bellvitge - IDIBELLAndalusian Center for Developmental Biology (CABD), Pablo de Olavide University/CSIC/Junta de AndalucíaAndalusian Center for Developmental Biology (CABD), Pablo de Olavide University/CSIC/Junta de AndalucíaModeling human diseases in C. elegans Group; Genes, Disease and Therapy Program, Institut d’Investigació Biomèdica de Bellvitge - IDIBELLDepartment of Genetics, Yale University School of MedicineModeling human diseases in C. elegans Group; Genes, Disease and Therapy Program, Institut d’Investigació Biomèdica de Bellvitge - IDIBELLModeling human diseases in C. elegans Group; Genes, Disease and Therapy Program, Institut d’Investigació Biomèdica de Bellvitge - IDIBELLModeling human diseases in C. elegans Group; Genes, Disease and Therapy Program, Institut d’Investigació Biomèdica de Bellvitge - IDIBELLAndalusian Center for Developmental Biology (CABD), Pablo de Olavide University/CSIC/Junta de AndalucíaBiomolecular Screening and Protein Technologies Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and TechnologyAndalusian Center for Developmental Biology (CABD), Pablo de Olavide University/CSIC/Junta de AndalucíaBiomolecular Screening and Protein Technologies Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and TechnologyAndalusian Center for Developmental Biology (CABD), Pablo de Olavide University/CSIC/Junta de AndalucíaDepartment of Genetics, Yale University School of MedicineCenter for Genomic Medicine, Massachusetts General HospitalModeling human diseases in C. elegans Group; Genes, Disease and Therapy Program, Institut d’Investigació Biomèdica de Bellvitge - IDIBELLAndalusian Center for Developmental Biology (CABD), Pablo de Olavide University/CSIC/Junta de AndalucíaPAM requirement is a constraint for genome editing but this has been circumvented by engineered Cas9 nucleases as SpG and SpRY recognizing minimal PAM sequences. Here, the authors validate and optimize SpG and SpRY in vivo expanding the targeting landscape in animals.https://doi.org/10.1038/s41467-022-30228-4 |
| spellingShingle | Jeremy Vicencio Carlos Sánchez-Bolaños Ismael Moreno-Sánchez David Brena Charles E. Vejnar Dmytro Kukhtar Miguel Ruiz-López Mariona Cots-Ponjoan Alejandro Rubio Natalia Rodrigo Melero Jesús Crespo-Cuadrado Carlo Carolis Antonio J. Pérez-Pulido Antonio J. Giráldez Benjamin P. Kleinstiver Julián Cerón Miguel A. Moreno-Mateos Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes Nature Communications |
| title | Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes |
| title_full | Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes |
| title_fullStr | Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes |
| title_full_unstemmed | Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes |
| title_short | Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes |
| title_sort | genome editing in animals with minimal pam crispr cas9 enzymes |
| url | https://doi.org/10.1038/s41467-022-30228-4 |
| work_keys_str_mv | AT jeremyvicencio genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT carlossanchezbolanos genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT ismaelmorenosanchez genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT davidbrena genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT charlesevejnar genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT dmytrokukhtar genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT miguelruizlopez genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT marionacotsponjoan genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT alejandrorubio genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT nataliarodrigomelero genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT jesuscrespocuadrado genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT carlocarolis genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT antoniojperezpulido genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT antoniojgiraldez genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT benjaminpkleinstiver genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT julianceron genomeeditinginanimalswithminimalpamcrisprcas9enzymes AT miguelamorenomateos genomeeditinginanimalswithminimalpamcrisprcas9enzymes |