Chromatin Morphology in Human Germinal Vesicle Oocytes and Their Competence to Mature in Stimulated Cycles
The search for simple morphological predictors of oocyte quality is an important task for assisted reproduction technologies (ARTs). One such predictor may be the morphology of the oocyte nucleus, called the germinal vesicle (GV), including the level of chromatin aggregation around the atypical nucl...
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2023-07-01
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| author | Daniil Salimov Tatiana Lisovskaya Junko Otsuki Alexandre Gzgzyan Irina Bogolyubova Dmitry Bogolyubov |
| author_facet | Daniil Salimov Tatiana Lisovskaya Junko Otsuki Alexandre Gzgzyan Irina Bogolyubova Dmitry Bogolyubov |
| author_sort | Daniil Salimov |
| collection | DOAJ |
| description | The search for simple morphological predictors of oocyte quality is an important task for assisted reproduction technologies (ARTs). One such predictor may be the morphology of the oocyte nucleus, called the germinal vesicle (GV), including the level of chromatin aggregation around the atypical nucleolus (ANu)—a peculiar nuclear organelle, formerly referred to as the nucleolus-like body. A prospective cohort study allowed distinguishing three classes of GV oocytes among 135 oocytes retrieved from 64 patients: with a non-surrounded ANu and rare chromatin blocks in the nucleoplasm (Class A), with a complete peri-ANu heterochromatic rim assembling all chromatin (Class C), and intermediate variants (Class B). Comparison of the chromatin state and the ability of oocytes to complete meiosis allowed us to conclude that Class B and C oocytes are more capable of resuming meiosis in vitro and completing the first meiotic division, while Class A oocytes can resume maturation but often stop their development either at metaphase I (MI arrest) or before the onset of GV breakdown (GVBD arrest). In addition, oocytes with a low chromatin condensation demonstrated a high level of aneuploidy during the resumption of meiosis. Considering that the degree of chromatin condensation/compaction can be determined in vivo under a light microscope, this characteristic of the GV can be considered a promising criterion for selecting the best-quality GV oocytes in IVM rescue programs. |
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| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T00:29:24Z |
| publishDate | 2023-07-01 |
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| spelling | doaj.art-65b033a26339493481418ad1c78289ee2023-11-18T22:45:11ZengMDPI AGCells2073-44092023-07-011215197610.3390/cells12151976Chromatin Morphology in Human Germinal Vesicle Oocytes and Their Competence to Mature in Stimulated CyclesDaniil Salimov0Tatiana Lisovskaya1Junko Otsuki2Alexandre Gzgzyan3Irina Bogolyubova4Dmitry Bogolyubov5Clinical Institute of Reproductive Medicine, Yekaterinburg 620014, RussiaClinical Institute of Reproductive Medicine, Yekaterinburg 620014, RussiaAssisted Reproductive Technology Center, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University, Okayama 700-8530, JapanResearch Institute of Obstetrics, Gynecology and Reproductology Named after D. O. Ott, St. Petersburg 199034, RussiaInstitute of Cytology, Russian Academy of Sciences, St. Petersburg 194064, RussiaInstitute of Cytology, Russian Academy of Sciences, St. Petersburg 194064, RussiaThe search for simple morphological predictors of oocyte quality is an important task for assisted reproduction technologies (ARTs). One such predictor may be the morphology of the oocyte nucleus, called the germinal vesicle (GV), including the level of chromatin aggregation around the atypical nucleolus (ANu)—a peculiar nuclear organelle, formerly referred to as the nucleolus-like body. A prospective cohort study allowed distinguishing three classes of GV oocytes among 135 oocytes retrieved from 64 patients: with a non-surrounded ANu and rare chromatin blocks in the nucleoplasm (Class A), with a complete peri-ANu heterochromatic rim assembling all chromatin (Class C), and intermediate variants (Class B). Comparison of the chromatin state and the ability of oocytes to complete meiosis allowed us to conclude that Class B and C oocytes are more capable of resuming meiosis in vitro and completing the first meiotic division, while Class A oocytes can resume maturation but often stop their development either at metaphase I (MI arrest) or before the onset of GV breakdown (GVBD arrest). In addition, oocytes with a low chromatin condensation demonstrated a high level of aneuploidy during the resumption of meiosis. Considering that the degree of chromatin condensation/compaction can be determined in vivo under a light microscope, this characteristic of the GV can be considered a promising criterion for selecting the best-quality GV oocytes in IVM rescue programs.https://www.mdpi.com/2073-4409/12/15/1976human oocytegerminal vesiclespontaneous maturationstimulated cycleaneuploidy rateatypical nucleolus |
| spellingShingle | Daniil Salimov Tatiana Lisovskaya Junko Otsuki Alexandre Gzgzyan Irina Bogolyubova Dmitry Bogolyubov Chromatin Morphology in Human Germinal Vesicle Oocytes and Their Competence to Mature in Stimulated Cycles Cells human oocyte germinal vesicle spontaneous maturation stimulated cycle aneuploidy rate atypical nucleolus |
| title | Chromatin Morphology in Human Germinal Vesicle Oocytes and Their Competence to Mature in Stimulated Cycles |
| title_full | Chromatin Morphology in Human Germinal Vesicle Oocytes and Their Competence to Mature in Stimulated Cycles |
| title_fullStr | Chromatin Morphology in Human Germinal Vesicle Oocytes and Their Competence to Mature in Stimulated Cycles |
| title_full_unstemmed | Chromatin Morphology in Human Germinal Vesicle Oocytes and Their Competence to Mature in Stimulated Cycles |
| title_short | Chromatin Morphology in Human Germinal Vesicle Oocytes and Their Competence to Mature in Stimulated Cycles |
| title_sort | chromatin morphology in human germinal vesicle oocytes and their competence to mature in stimulated cycles |
| topic | human oocyte germinal vesicle spontaneous maturation stimulated cycle aneuploidy rate atypical nucleolus |
| url | https://www.mdpi.com/2073-4409/12/15/1976 |
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