Dynamic response of Aspergillus niger to single pulses of glucose with high and low concentrations
Abstract Microorganisms generally encounter a fluctuating environment in their natural habitat and similar conditions also happen in large-scale bioreactors. In this work, the dynamic response of intracellular and extracellular metabolites of Aspergillus niger was investigated after sudden exposure...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-05-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40643-019-0251-y |
| _version_ | 1828313161135030272 |
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| author | Shuai Wang Peng Liu Wei Shu Chao Li Huan Li Shanshan Liu Jianye Xia Henk Noorman |
| author_facet | Shuai Wang Peng Liu Wei Shu Chao Li Huan Li Shanshan Liu Jianye Xia Henk Noorman |
| author_sort | Shuai Wang |
| collection | DOAJ |
| description | Abstract Microorganisms generally encounter a fluctuating environment in their natural habitat and similar conditions also happen in large-scale bioreactors. In this work, the dynamic response of intracellular and extracellular metabolites of Aspergillus niger was investigated after sudden exposure to high and low excess glucose concentrations in chemostats. It was found that the steady-state pathway turnover time of the carbon flux through the central carbon metabolism (CCM) was PP pathway 50 s, EMP pathway 20 s, and TCA cycle 189 s, and an upper limit for individual metabolite concentrations in the CCM was estimated. Regardless of the glucose pulse size, little changes of amino acids levels were observed except for aspartate, which showed a significant decrease. The ATP paradox, known from other organisms, was also observed in the studied A. niger strain. However, a different response of the NAD+/NADH ratio to the glucose pulses was found in A. niger compared to previously published observations on Penicillium chrysogenum and Saccharomyces cerevisiae. These findings are valuable for better understanding A. niger culture performance in large-scale bioreactors. |
| first_indexed | 2024-04-13T16:26:48Z |
| format | Article |
| id | doaj.art-65b0a324edce4fe0a930ec0463933648 |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-04-13T16:26:48Z |
| publishDate | 2019-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-65b0a324edce4fe0a930ec04639336482022-12-22T02:39:43ZengSpringerOpenBioresources and Bioprocessing2197-43652019-05-016111410.1186/s40643-019-0251-yDynamic response of Aspergillus niger to single pulses of glucose with high and low concentrationsShuai Wang0Peng Liu1Wei Shu2Chao Li3Huan Li4Shanshan Liu5Jianye Xia6Henk Noorman7State Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyDSM Biotechnology CenterAbstract Microorganisms generally encounter a fluctuating environment in their natural habitat and similar conditions also happen in large-scale bioreactors. In this work, the dynamic response of intracellular and extracellular metabolites of Aspergillus niger was investigated after sudden exposure to high and low excess glucose concentrations in chemostats. It was found that the steady-state pathway turnover time of the carbon flux through the central carbon metabolism (CCM) was PP pathway 50 s, EMP pathway 20 s, and TCA cycle 189 s, and an upper limit for individual metabolite concentrations in the CCM was estimated. Regardless of the glucose pulse size, little changes of amino acids levels were observed except for aspartate, which showed a significant decrease. The ATP paradox, known from other organisms, was also observed in the studied A. niger strain. However, a different response of the NAD+/NADH ratio to the glucose pulses was found in A. niger compared to previously published observations on Penicillium chrysogenum and Saccharomyces cerevisiae. These findings are valuable for better understanding A. niger culture performance in large-scale bioreactors.http://link.springer.com/article/10.1186/s40643-019-0251-yAspergillus nigerSubstrate pulseGlucose excessRapid samplingDynamic responsesIntracellular metabolites |
| spellingShingle | Shuai Wang Peng Liu Wei Shu Chao Li Huan Li Shanshan Liu Jianye Xia Henk Noorman Dynamic response of Aspergillus niger to single pulses of glucose with high and low concentrations Bioresources and Bioprocessing Aspergillus niger Substrate pulse Glucose excess Rapid sampling Dynamic responses Intracellular metabolites |
| title | Dynamic response of Aspergillus niger to single pulses of glucose with high and low concentrations |
| title_full | Dynamic response of Aspergillus niger to single pulses of glucose with high and low concentrations |
| title_fullStr | Dynamic response of Aspergillus niger to single pulses of glucose with high and low concentrations |
| title_full_unstemmed | Dynamic response of Aspergillus niger to single pulses of glucose with high and low concentrations |
| title_short | Dynamic response of Aspergillus niger to single pulses of glucose with high and low concentrations |
| title_sort | dynamic response of aspergillus niger to single pulses of glucose with high and low concentrations |
| topic | Aspergillus niger Substrate pulse Glucose excess Rapid sampling Dynamic responses Intracellular metabolites |
| url | http://link.springer.com/article/10.1186/s40643-019-0251-y |
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