Intravital Imaging Allows Organ-Specific Insights Into Immune Functions
Leukocytes are among the most mobile and versatile cells that have many essential functions in homeostasis and survival. Especially cells from the innate immune system, i.e., neutrophils and macrophages, play an important role as rapid first responders against invading microorganisms. With the adven...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.623906/full |
| _version_ | 1818458602465132544 |
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| author | Selina K. Jorch Carsten Deppermann |
| author_facet | Selina K. Jorch Carsten Deppermann |
| author_sort | Selina K. Jorch |
| collection | DOAJ |
| description | Leukocytes are among the most mobile and versatile cells that have many essential functions in homeostasis and survival. Especially cells from the innate immune system, i.e., neutrophils and macrophages, play an important role as rapid first responders against invading microorganisms. With the advent of novel imaging techniques, new ways of visualizing innate immune cells have become available in recent years, thereby enabling more and more detailed discoveries about their nature, function and interaction partners. Besides intravital spinning-disc and 2-photon microscopy, clearing and 3D-imaging techniques provide new insights into the mechanism of innate immune cell behavior in their natural environment. This mini review focuses on the contributions of novel-imaging techniques to provide insight into the functions of neutrophils and macrophages under homeostasis and in infections. Imaging setups for different organs like the liver, kidney, heart, lung, and the peritoneal cavity are discussed as well as the current limitations of these imaging techniques. |
| first_indexed | 2024-12-14T23:01:04Z |
| format | Article |
| id | doaj.art-65b274835fa84010919ee4a022914f5b |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-14T23:01:04Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-65b274835fa84010919ee4a022914f5b2022-12-21T22:44:27ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-02-01910.3389/fcell.2021.623906623906Intravital Imaging Allows Organ-Specific Insights Into Immune FunctionsSelina K. Jorch0Carsten Deppermann1Institute of Experimental Immunology, University Hospital of Bonn, Rheinische Friedrich-Wilhelms Universität, Bonn, GermanyInstitute of Clinical Chemistry and Laboratory Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, GermanyLeukocytes are among the most mobile and versatile cells that have many essential functions in homeostasis and survival. Especially cells from the innate immune system, i.e., neutrophils and macrophages, play an important role as rapid first responders against invading microorganisms. With the advent of novel imaging techniques, new ways of visualizing innate immune cells have become available in recent years, thereby enabling more and more detailed discoveries about their nature, function and interaction partners. Besides intravital spinning-disc and 2-photon microscopy, clearing and 3D-imaging techniques provide new insights into the mechanism of innate immune cell behavior in their natural environment. This mini review focuses on the contributions of novel-imaging techniques to provide insight into the functions of neutrophils and macrophages under homeostasis and in infections. Imaging setups for different organs like the liver, kidney, heart, lung, and the peritoneal cavity are discussed as well as the current limitations of these imaging techniques.https://www.frontiersin.org/articles/10.3389/fcell.2021.623906/fullIntravital microscopyliverperitoneal cavitylungkidneymacrophage |
| spellingShingle | Selina K. Jorch Carsten Deppermann Intravital Imaging Allows Organ-Specific Insights Into Immune Functions Frontiers in Cell and Developmental Biology Intravital microscopy liver peritoneal cavity lung kidney macrophage |
| title | Intravital Imaging Allows Organ-Specific Insights Into Immune Functions |
| title_full | Intravital Imaging Allows Organ-Specific Insights Into Immune Functions |
| title_fullStr | Intravital Imaging Allows Organ-Specific Insights Into Immune Functions |
| title_full_unstemmed | Intravital Imaging Allows Organ-Specific Insights Into Immune Functions |
| title_short | Intravital Imaging Allows Organ-Specific Insights Into Immune Functions |
| title_sort | intravital imaging allows organ specific insights into immune functions |
| topic | Intravital microscopy liver peritoneal cavity lung kidney macrophage |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.623906/full |
| work_keys_str_mv | AT selinakjorch intravitalimagingallowsorganspecificinsightsintoimmunefunctions AT carstendeppermann intravitalimagingallowsorganspecificinsightsintoimmunefunctions |