Prevalence, toxigenic potential and antimicrobial susceptibility profile of Staphylococcus isolated from ready-to-eat meats
Aim: An epidemiological surveillance for Staphylococci contamination of ready-to-eat (RTE) meats from Enugu State, Nigeria, was carried out to determine the prevalence, species distribution, toxigenic potential and antimicrobial susceptibility profile of the organisms and hence the microbiological a...
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| Format: | Article |
| Language: | English |
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Veterinary World
2018-09-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.11/September-2018/3.pdf |
| _version_ | 1818717542402752512 |
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| author | Chinwe E. Okoli Emmanuel Okechukwu Njoga Simon I. Enem Enid E. Godwin John A. Nwanta Kennedy F. Chah |
| author_facet | Chinwe E. Okoli Emmanuel Okechukwu Njoga Simon I. Enem Enid E. Godwin John A. Nwanta Kennedy F. Chah |
| author_sort | Chinwe E. Okoli |
| collection | DOAJ |
| description | Aim: An epidemiological surveillance for Staphylococci contamination of ready-to-eat (RTE) meats from Enugu State, Nigeria, was carried out to determine the prevalence, species distribution, toxigenic potential and antimicrobial susceptibility profile of the organisms and hence the microbiological and toxicological safety of the meats.
Materials and Methods: Isolation and phenotypic Staphylococcus detection were done according to standard microbiological methods. Phenotypic resistance to 17 commonly used antimicrobial agents was determined by disc diffusion method. Molecular characterization of the isolates to species level and detection of selected toxigenic and antimicrobial-resistance genes were done by PCR methods.
Results: Twenty-four (9.4%) of the 255 meat samples investigated were contaminated with Staphylococcus species. Twenty-four Staphylococcus isolates belonging to six species of coagulase-negative Staphylococcus (CoNS) were identified. Four (16.7%) isolates harbored genes coding for exfoliative toxin-A. Ten (41.7%) isolates were multidrug resistant, while mecA, tetK, mphC, ermT and ermC were the antimicrobial-resistance genes detected in the isolates. Meat samples sourced from motor parks (16.7%) and open markets (8.5%) were the most contaminated.
Conclusion: 9.4% of RTE meats sampled were contaminated with toxigenic and multidrug resistance CoNS. Beef was the most contaminated RTE meat type and harbored all the toxigenic and most of the antibiotic-resistant genes detected. Meat samples from motor parks had the highest staphylococcal contamination (16.7%), while those from mechanic village had the least (2.4%). Majority (79.2%) of the isolates were not susceptible to fusidic acid but none exhibited antimicrobial-resistance to chloramphenicol, ciprofloxacin, linezolid or teicoplanin. Food safety authorities in the study area should work proactively to massively improve the hygienic practices of meat vendors; in order to limit staphylococcal contamination of RTE meats and the associated public health problems. |
| first_indexed | 2024-12-17T19:36:48Z |
| format | Article |
| id | doaj.art-65bac4e6886c41bdb5dcd332e2d436c1 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-17T19:36:48Z |
| publishDate | 2018-09-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-65bac4e6886c41bdb5dcd332e2d436c12022-12-21T21:35:07ZengVeterinary WorldVeterinary World0972-89882231-09162018-09-011191214122110.14202/vetworld.2018.1214-1221Prevalence, toxigenic potential and antimicrobial susceptibility profile of Staphylococcus isolated from ready-to-eat meatsChinwe E. Okoli0Emmanuel Okechukwu Njoga1Simon I. Enem2Enid E. Godwin3John A. Nwanta4Kennedy F. Chah5Department of Veterinary Public Health and Preventive Medicine, University of Abuja, Nigeria.Department of Veterinary Public Health and Preventive Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria.Department of Veterinary Public Health and Preventive Medicine, University of Abuja, Nigeria.Department of Veterinary Public Health and Preventive Medicine, University of Abuja, Nigeria.Department of Veterinary Public Health and Preventive Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria.Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria.Aim: An epidemiological surveillance for Staphylococci contamination of ready-to-eat (RTE) meats from Enugu State, Nigeria, was carried out to determine the prevalence, species distribution, toxigenic potential and antimicrobial susceptibility profile of the organisms and hence the microbiological and toxicological safety of the meats. Materials and Methods: Isolation and phenotypic Staphylococcus detection were done according to standard microbiological methods. Phenotypic resistance to 17 commonly used antimicrobial agents was determined by disc diffusion method. Molecular characterization of the isolates to species level and detection of selected toxigenic and antimicrobial-resistance genes were done by PCR methods. Results: Twenty-four (9.4%) of the 255 meat samples investigated were contaminated with Staphylococcus species. Twenty-four Staphylococcus isolates belonging to six species of coagulase-negative Staphylococcus (CoNS) were identified. Four (16.7%) isolates harbored genes coding for exfoliative toxin-A. Ten (41.7%) isolates were multidrug resistant, while mecA, tetK, mphC, ermT and ermC were the antimicrobial-resistance genes detected in the isolates. Meat samples sourced from motor parks (16.7%) and open markets (8.5%) were the most contaminated. Conclusion: 9.4% of RTE meats sampled were contaminated with toxigenic and multidrug resistance CoNS. Beef was the most contaminated RTE meat type and harbored all the toxigenic and most of the antibiotic-resistant genes detected. Meat samples from motor parks had the highest staphylococcal contamination (16.7%), while those from mechanic village had the least (2.4%). Majority (79.2%) of the isolates were not susceptible to fusidic acid but none exhibited antimicrobial-resistance to chloramphenicol, ciprofloxacin, linezolid or teicoplanin. Food safety authorities in the study area should work proactively to massively improve the hygienic practices of meat vendors; in order to limit staphylococcal contamination of RTE meats and the associated public health problems.http://www.veterinaryworld.org/Vol.11/September-2018/3.pdfantibiotic resistancefood safetyNigeriapolymerase chain reactionready-to-eat meatsStaphylococcus |
| spellingShingle | Chinwe E. Okoli Emmanuel Okechukwu Njoga Simon I. Enem Enid E. Godwin John A. Nwanta Kennedy F. Chah Prevalence, toxigenic potential and antimicrobial susceptibility profile of Staphylococcus isolated from ready-to-eat meats Veterinary World antibiotic resistance food safety Nigeria polymerase chain reaction ready-to-eat meats Staphylococcus |
| title | Prevalence, toxigenic potential and antimicrobial susceptibility profile of Staphylococcus isolated from ready-to-eat meats |
| title_full | Prevalence, toxigenic potential and antimicrobial susceptibility profile of Staphylococcus isolated from ready-to-eat meats |
| title_fullStr | Prevalence, toxigenic potential and antimicrobial susceptibility profile of Staphylococcus isolated from ready-to-eat meats |
| title_full_unstemmed | Prevalence, toxigenic potential and antimicrobial susceptibility profile of Staphylococcus isolated from ready-to-eat meats |
| title_short | Prevalence, toxigenic potential and antimicrobial susceptibility profile of Staphylococcus isolated from ready-to-eat meats |
| title_sort | prevalence toxigenic potential and antimicrobial susceptibility profile of staphylococcus isolated from ready to eat meats |
| topic | antibiotic resistance food safety Nigeria polymerase chain reaction ready-to-eat meats Staphylococcus |
| url | http://www.veterinaryworld.org/Vol.11/September-2018/3.pdf |
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