Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis
Abstract Bacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16–C17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture conditions. This work addressed the elucidation of the genome sequence of MEP218...
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| Format: | Article |
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Nature Portfolio
2023-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-49194-y |
| _version_ | 1797388342718889984 |
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| author | Daniela Medeot Analía Sannazzaro María Julia Estrella Gonzalo Torres Tejerizo Bruno Contreras-Moreira Mariano Pistorio Edgardo Jofré |
| author_facet | Daniela Medeot Analía Sannazzaro María Julia Estrella Gonzalo Torres Tejerizo Bruno Contreras-Moreira Mariano Pistorio Edgardo Jofré |
| author_sort | Daniela Medeot |
| collection | DOAJ |
| description | Abstract Bacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16–C17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture conditions. This work addressed the elucidation of the genome sequence of MEP218 and its taxonomic classification. The genome comprises 3,944,892 bp, with a total of 3474 coding sequences and a G + C content of 46.59%. Our phylogenetic analysis to determine the taxonomic position demonstrated that the assignment of the MEP218 strain to Bacillus velezensis species provides insights into its evolutionary context and potential functional attributes. The in silico genome analysis revealed eleven gene clusters involved in the synthesis of secondary metabolites, including non-ribosomal CLP (fengycins and surfactin), polyketides, terpenes, and bacteriocins. Furthermore, genes encoding phytase, involved in the release of phytic phosphate for plant and animal nutrition, or other enzymes such as cellulase, xylanase, and alpha 1–4 glucanase were detected. In vitro antagonistic assays against Salmonella typhimurium, Acinetobacter baumanii, Escherichia coli, among others, demonstrated a broad spectrum of C16–C17 fengycin produced by MEP218. MEP218 genome sequence analysis expanded our understanding of the diversity and genetic relationships within the Bacillus genus and updated the Bacillus databases with its unique trait to produce antibacterial fengycins and its potential as a resource of biotechnologically useful enzymes. |
| first_indexed | 2024-03-08T22:38:23Z |
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| id | doaj.art-65bb1c0b1b474a4e9316ef548363f2f6 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T22:38:23Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-65bb1c0b1b474a4e9316ef548363f2f62023-12-17T12:17:56ZengNature PortfolioScientific Reports2045-23222023-12-0113111410.1038/s41598-023-49194-yUnraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysisDaniela Medeot0Analía Sannazzaro1María Julia Estrella2Gonzalo Torres Tejerizo3Bruno Contreras-Moreira4Mariano Pistorio5Edgardo Jofré6Instituto de Biotecnología Ambiental y Salud (INBIAS), CCT-CONICET-Córdoba, Universidad Nacional de Río CuartoInstituto Tecnológico de Chascomús (INTECH), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de San Martín (UNSAM)Instituto Tecnológico de Chascomús (INTECH), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de San Martín (UNSAM)Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, IBBM (Instituto de Biotecnología y Biología Molecular), CCT-CONICET-La Plata, Universidad Nacional de La PlataEstación Experimental de Aula Dei-CSICDepartamento de Ciencias Biológicas, Facultad de Ciencias Exactas, IBBM (Instituto de Biotecnología y Biología Molecular), CCT-CONICET-La Plata, Universidad Nacional de La PlataInstituto de Biotecnología Ambiental y Salud (INBIAS), CCT-CONICET-Córdoba, Universidad Nacional de Río CuartoAbstract Bacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16–C17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture conditions. This work addressed the elucidation of the genome sequence of MEP218 and its taxonomic classification. The genome comprises 3,944,892 bp, with a total of 3474 coding sequences and a G + C content of 46.59%. Our phylogenetic analysis to determine the taxonomic position demonstrated that the assignment of the MEP218 strain to Bacillus velezensis species provides insights into its evolutionary context and potential functional attributes. The in silico genome analysis revealed eleven gene clusters involved in the synthesis of secondary metabolites, including non-ribosomal CLP (fengycins and surfactin), polyketides, terpenes, and bacteriocins. Furthermore, genes encoding phytase, involved in the release of phytic phosphate for plant and animal nutrition, or other enzymes such as cellulase, xylanase, and alpha 1–4 glucanase were detected. In vitro antagonistic assays against Salmonella typhimurium, Acinetobacter baumanii, Escherichia coli, among others, demonstrated a broad spectrum of C16–C17 fengycin produced by MEP218. MEP218 genome sequence analysis expanded our understanding of the diversity and genetic relationships within the Bacillus genus and updated the Bacillus databases with its unique trait to produce antibacterial fengycins and its potential as a resource of biotechnologically useful enzymes.https://doi.org/10.1038/s41598-023-49194-y |
| spellingShingle | Daniela Medeot Analía Sannazzaro María Julia Estrella Gonzalo Torres Tejerizo Bruno Contreras-Moreira Mariano Pistorio Edgardo Jofré Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis Scientific Reports |
| title | Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis |
| title_full | Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis |
| title_fullStr | Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis |
| title_full_unstemmed | Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis |
| title_short | Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis |
| title_sort | unraveling the genome of bacillus velezensis mep218 a strain producing fengycin homologs with broad antibacterial activity comprehensive comparative genome analysis |
| url | https://doi.org/10.1038/s41598-023-49194-y |
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