Corn Stover Pretreatment with Na<sub>2</sub>CO<sub>3</sub> Solution from Absorption of Recovered CO<sub>2</sub>
Renewable resources such as lignocellulosic biomass are effective at producing fermentable sugars during enzymatic hydrolysis when pretreated. Optimizing pretreatment methods for delignification while maintaining sustainability and low processing costs requires innovative strategies such as reusing...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/11/600 |
| _version_ | 1797468298952048640 |
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| author | Valerie García-Negrón Matthew J. Toht |
| author_facet | Valerie García-Negrón Matthew J. Toht |
| author_sort | Valerie García-Negrón |
| collection | DOAJ |
| description | Renewable resources such as lignocellulosic biomass are effective at producing fermentable sugars during enzymatic hydrolysis when pretreated. Optimizing pretreatment methods for delignification while maintaining sustainability and low processing costs requires innovative strategies such as reusing greenhouse gas emissions for materials processing. Corn stover, an agricultural waste residue, was pretreated with 2.2 M <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi mathvariant="normal">N</mi><msub><mi mathvariant="normal">a</mi><mn>2</mn></msub><mi mathvariant="normal">C</mi><msub><mi mathvariant="normal">O</mi><mn>3</mn></msub></mrow></semantics></math></inline-formula> produced from <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi mathvariant="normal">C</mi><msub><mi mathvariant="normal">O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula> captured via absorption in a 5 M NaOH solution. Composition analysis of the pretreated corn stover exhibited higher cellulose content (40.96%) and less lignin (16.50%) than the untreated biomass. Changes in the chemical structures are visible in the FTIR-ATR spectra, particularly in the cellulose and lignin-related absorption bands. The sugar release from hydrolysis was evaluated at different time intervals and by varying two enzyme ratios of CTec2-to-HTec2 (2:1 and 3:1). Enzymatic hydrolysis produced higher and more stable glucose yields for the pretreated biomass, surpassing 90% after 24 h using the 3:1 enzyme ratio. Sugar concentrations notably increased after pretreatment and even more when using the cellulase-rich enzyme solution. The maximum glucose, xylose, and arabinose recovered were 44, 19, and 2.3 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi mathvariant="normal">g</mi><mo> </mo><mi mathvariant="normal">L</mi></mrow></semantics></math></inline-formula><sup>−1</sup>. These results demonstrate the viability of capturing <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi mathvariant="normal">C</mi><msub><mi mathvariant="normal">O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula> and converting it into an efficient <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi mathvariant="normal">N</mi><msub><mi mathvariant="normal">a</mi><mn>2</mn></msub><mi mathvariant="normal">C</mi><msub><mi mathvariant="normal">O</mi><mn>3</mn></msub></mrow></semantics></math></inline-formula> pretreatment for corn stover biomass. Additional processing optimizations depend on the combination of physicochemical parameters selected. |
| first_indexed | 2024-03-09T19:05:27Z |
| format | Article |
| id | doaj.art-65bbeaff7a594dcbb32ab77b98700961 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T19:05:27Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-65bbeaff7a594dcbb32ab77b987009612023-11-24T04:38:05ZengMDPI AGFermentation2311-56372022-11-0181160010.3390/fermentation8110600Corn Stover Pretreatment with Na<sub>2</sub>CO<sub>3</sub> Solution from Absorption of Recovered CO<sub>2</sub>Valerie García-Negrón0Matthew J. Toht1Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USAEastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USARenewable resources such as lignocellulosic biomass are effective at producing fermentable sugars during enzymatic hydrolysis when pretreated. Optimizing pretreatment methods for delignification while maintaining sustainability and low processing costs requires innovative strategies such as reusing greenhouse gas emissions for materials processing. Corn stover, an agricultural waste residue, was pretreated with 2.2 M <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi mathvariant="normal">N</mi><msub><mi mathvariant="normal">a</mi><mn>2</mn></msub><mi mathvariant="normal">C</mi><msub><mi mathvariant="normal">O</mi><mn>3</mn></msub></mrow></semantics></math></inline-formula> produced from <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi mathvariant="normal">C</mi><msub><mi mathvariant="normal">O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula> captured via absorption in a 5 M NaOH solution. Composition analysis of the pretreated corn stover exhibited higher cellulose content (40.96%) and less lignin (16.50%) than the untreated biomass. Changes in the chemical structures are visible in the FTIR-ATR spectra, particularly in the cellulose and lignin-related absorption bands. The sugar release from hydrolysis was evaluated at different time intervals and by varying two enzyme ratios of CTec2-to-HTec2 (2:1 and 3:1). Enzymatic hydrolysis produced higher and more stable glucose yields for the pretreated biomass, surpassing 90% after 24 h using the 3:1 enzyme ratio. Sugar concentrations notably increased after pretreatment and even more when using the cellulase-rich enzyme solution. The maximum glucose, xylose, and arabinose recovered were 44, 19, and 2.3 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi mathvariant="normal">g</mi><mo> </mo><mi mathvariant="normal">L</mi></mrow></semantics></math></inline-formula><sup>−1</sup>. These results demonstrate the viability of capturing <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi mathvariant="normal">C</mi><msub><mi mathvariant="normal">O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula> and converting it into an efficient <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi mathvariant="normal">N</mi><msub><mi mathvariant="normal">a</mi><mn>2</mn></msub><mi mathvariant="normal">C</mi><msub><mi mathvariant="normal">O</mi><mn>3</mn></msub></mrow></semantics></math></inline-formula> pretreatment for corn stover biomass. Additional processing optimizations depend on the combination of physicochemical parameters selected.https://www.mdpi.com/2311-5637/8/11/600corn stovercarbon dioxide absorptionbiomass pretreatmentsodium carbonatesodium hydroxide |
| spellingShingle | Valerie García-Negrón Matthew J. Toht Corn Stover Pretreatment with Na<sub>2</sub>CO<sub>3</sub> Solution from Absorption of Recovered CO<sub>2</sub> Fermentation corn stover carbon dioxide absorption biomass pretreatment sodium carbonate sodium hydroxide |
| title | Corn Stover Pretreatment with Na<sub>2</sub>CO<sub>3</sub> Solution from Absorption of Recovered CO<sub>2</sub> |
| title_full | Corn Stover Pretreatment with Na<sub>2</sub>CO<sub>3</sub> Solution from Absorption of Recovered CO<sub>2</sub> |
| title_fullStr | Corn Stover Pretreatment with Na<sub>2</sub>CO<sub>3</sub> Solution from Absorption of Recovered CO<sub>2</sub> |
| title_full_unstemmed | Corn Stover Pretreatment with Na<sub>2</sub>CO<sub>3</sub> Solution from Absorption of Recovered CO<sub>2</sub> |
| title_short | Corn Stover Pretreatment with Na<sub>2</sub>CO<sub>3</sub> Solution from Absorption of Recovered CO<sub>2</sub> |
| title_sort | corn stover pretreatment with na sub 2 sub co sub 3 sub solution from absorption of recovered co sub 2 sub |
| topic | corn stover carbon dioxide absorption biomass pretreatment sodium carbonate sodium hydroxide |
| url | https://www.mdpi.com/2311-5637/8/11/600 |
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