Factor V Leiden and Prothrombin Mutations in South of Iran
ABSTRACT: Introduction & Objective: Factor V Leiden and prothrombin mutation are not common but they are involved in pediatric thrombosis. The aim of this study was to evaluate the frequency of factor V Leiden & prohtrombin mutation in healthy population of Shiraz, south of Iran. Materials...
| Main Authors: | , , |
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| Format: | Article |
| Language: | fas |
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Yasuj University Of Medical Sciences
2007-01-01
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| Series: | Armaghane Danesh Bimonthly Journal |
| Subjects: | |
| Online Access: | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-39-81&slc_lang=en&sid=1 |
| _version_ | 1811236692087013376 |
|---|---|
| author | M Karimi AR Panahande AR Afrasiabi |
| author_facet | M Karimi AR Panahande AR Afrasiabi |
| author_sort | M Karimi |
| collection | DOAJ |
| description | ABSTRACT:
Introduction & Objective: Factor V Leiden and prothrombin mutation are not common but they are involved in pediatric thrombosis. The aim of this study was to evaluate the frequency of factor V Leiden & prohtrombin mutation in healthy population of Shiraz, south of Iran.
Materials & Methods: In this cross-sectional study 195 healthy people (97 female and 98 male) were randomly selected. Peripheral white blood cells obtained from 5 ml blood contained 1-2 mg/ml K2- EDTA. Genomic DNA extraction was performed following the protocol described by Miller et al. PCR amplification was carried out in 25μl reaction volume containing 0.5 units Taq polymerase, 200μM dNTP, 500 μM of each of the previously described primers. After initial denaturation, 35 cycles at 95◦c for 30s, and 72◦c for 20s and followed extention by 72 for 10 min were performed. About 10μl of PDR product was digested with MNI I or Mbo restriction enzymes.
Results: In this study we determined factor V Leiden in 8 (4.1%) and prothrombin mutation in 6 individual (3.07%) of 198 cases in heterozygous form. No homozygous was seen for any of the mutations. Only one case presented a double heterozygous for factor V and prothrombin in this cohort.
Conclusion: Several studies of factor V leiden and prothrombin mutations in the East of Asia showed the higher frequency of these mutations in Iran. |
| first_indexed | 2024-04-12T12:13:32Z |
| format | Article |
| id | doaj.art-65bf5bd78749441ebc9760d97ae80eb5 |
| institution | Directory Open Access Journal |
| issn | 1728-6506 1728-6514 |
| language | fas |
| last_indexed | 2024-04-12T12:13:32Z |
| publishDate | 2007-01-01 |
| publisher | Yasuj University Of Medical Sciences |
| record_format | Article |
| series | Armaghane Danesh Bimonthly Journal |
| spelling | doaj.art-65bf5bd78749441ebc9760d97ae80eb52022-12-22T03:33:31ZfasYasuj University Of Medical SciencesArmaghane Danesh Bimonthly Journal1728-65061728-65142007-01-011148188Factor V Leiden and Prothrombin Mutations in South of IranM Karimi0AR Panahande1AR Afrasiabi2 ABSTRACT: Introduction & Objective: Factor V Leiden and prothrombin mutation are not common but they are involved in pediatric thrombosis. The aim of this study was to evaluate the frequency of factor V Leiden & prohtrombin mutation in healthy population of Shiraz, south of Iran. Materials & Methods: In this cross-sectional study 195 healthy people (97 female and 98 male) were randomly selected. Peripheral white blood cells obtained from 5 ml blood contained 1-2 mg/ml K2- EDTA. Genomic DNA extraction was performed following the protocol described by Miller et al. PCR amplification was carried out in 25μl reaction volume containing 0.5 units Taq polymerase, 200μM dNTP, 500 μM of each of the previously described primers. After initial denaturation, 35 cycles at 95◦c for 30s, and 72◦c for 20s and followed extention by 72 for 10 min were performed. About 10μl of PDR product was digested with MNI I or Mbo restriction enzymes. Results: In this study we determined factor V Leiden in 8 (4.1%) and prothrombin mutation in 6 individual (3.07%) of 198 cases in heterozygous form. No homozygous was seen for any of the mutations. Only one case presented a double heterozygous for factor V and prothrombin in this cohort. Conclusion: Several studies of factor V leiden and prothrombin mutations in the East of Asia showed the higher frequency of these mutations in Iran.http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-39-81&slc_lang=en&sid=1KEYWORDS: Mutation Factor V Leiden Prothormbin |
| spellingShingle | M Karimi AR Panahande AR Afrasiabi Factor V Leiden and Prothrombin Mutations in South of Iran Armaghane Danesh Bimonthly Journal KEYWORDS: Mutation Factor V Leiden Prothormbin |
| title | Factor V Leiden and Prothrombin Mutations in South of Iran |
| title_full | Factor V Leiden and Prothrombin Mutations in South of Iran |
| title_fullStr | Factor V Leiden and Prothrombin Mutations in South of Iran |
| title_full_unstemmed | Factor V Leiden and Prothrombin Mutations in South of Iran |
| title_short | Factor V Leiden and Prothrombin Mutations in South of Iran |
| title_sort | factor v leiden and prothrombin mutations in south of iran |
| topic | KEYWORDS: Mutation Factor V Leiden Prothormbin |
| url | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-39-81&slc_lang=en&sid=1 |
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