Identification of the rice blast fungus, Magnaporthe oryzae by the polymerase chain reaction (PCR)
Magnaporthe oryzaeis a causal agent of the rice blast disease resulting serious damages in the production of rice worldwide. The early symptom of rice blast disease can be confused with those caused by other fungal diseases leading to many difficulties in efficient and effective disease management....
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE
2016-11-01
|
| Series: | Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ |
| Subjects: | |
| Online Access: | https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/823 |
| _version_ | 1819099764618166272 |
|---|---|
| author | Đoàn Thị Hòa Võ Thị Ngọc Linh Trương Thành Nhập Nguyễn Bằng Phi Nguyễn Ngọc Bảo Châu Nguyễn Bảo Quốc |
| author_facet | Đoàn Thị Hòa Võ Thị Ngọc Linh Trương Thành Nhập Nguyễn Bằng Phi Nguyễn Ngọc Bảo Châu Nguyễn Bảo Quốc |
| author_sort | Đoàn Thị Hòa |
| collection | DOAJ |
| description | Magnaporthe oryzaeis a causal agent of the rice blast disease resulting serious damages in the production of rice worldwide. The early symptom of rice blast disease can be confused with those caused by other fungal diseases leading to many difficulties in efficient and effective disease management. Currently, identification of the rice blast fungus by using conventional method requires more time (at least 24 hours), labour and unreliability. An alternative method by using polymerase chain reaction (PCR) with the primers of Pot2 transposon known to reduce those factors for detection of the rice blast fungus will be done in this study. The results indicated the specificity of Pot2 transposon can be used for the rapid detection of both Magnaporthe oryzae and blast fungus infected leaf/neck in rice, but not in other phytopathogenic fungi. Therefore, PCR method has been considered as an alternative tool of diagnostic, particularly in-field detection of blast disease in practice. |
| first_indexed | 2024-12-22T00:52:04Z |
| format | Article |
| id | doaj.art-65c02741dd794913a8576f93a5ee25e4 |
| institution | Directory Open Access Journal |
| issn | 2734-9322 2734-9594 |
| language | English |
| last_indexed | 2024-12-22T00:52:04Z |
| publishDate | 2016-11-01 |
| publisher | HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE |
| record_format | Article |
| series | Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ |
| spelling | doaj.art-65c02741dd794913a8576f93a5ee25e42022-12-21T18:44:25ZengHO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCETạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ2734-93222734-95942016-11-011111218695Identification of the rice blast fungus, Magnaporthe oryzae by the polymerase chain reaction (PCR)Đoàn Thị Hòa0Võ Thị Ngọc Linh1Trương Thành Nhập2Nguyễn Bằng Phi3Nguyễn Ngọc Bảo Châu4Nguyễn Bảo Quốc5Viện Nghiên Cứu Công Nghệ Sinh Học và Môi Trường, Đại học Nông Lâm TP. Hồ Chí MinhViện Nghiên Cứu Công Nghệ Sinh Học và Môi Trường, Đại học Nông Lâm TP. Hồ Chí MinhViện Nghiên Cứu Công Nghệ Sinh Học và Môi Trường, Đại học Nông Lâm TP. Hồ Chí MinhKhoa Tài Nguyên Môi Trường, Đại Học Thủ Dầu Một, Bình DươngTrường Đại học Mở Thành phố Hồ Chí MinhViện Nghiên Cứu Công Nghệ Sinh Học và Môi Trường, Đại học Nông Lâm TP. Hồ Chí MinhMagnaporthe oryzaeis a causal agent of the rice blast disease resulting serious damages in the production of rice worldwide. The early symptom of rice blast disease can be confused with those caused by other fungal diseases leading to many difficulties in efficient and effective disease management. Currently, identification of the rice blast fungus by using conventional method requires more time (at least 24 hours), labour and unreliability. An alternative method by using polymerase chain reaction (PCR) with the primers of Pot2 transposon known to reduce those factors for detection of the rice blast fungus will be done in this study. The results indicated the specificity of Pot2 transposon can be used for the rapid detection of both Magnaporthe oryzae and blast fungus infected leaf/neck in rice, but not in other phytopathogenic fungi. Therefore, PCR method has been considered as an alternative tool of diagnostic, particularly in-field detection of blast disease in practice.https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/823pcrnấm đạo ônbào tửpot2 transposonmagnaporthe oryzae |
| spellingShingle | Đoàn Thị Hòa Võ Thị Ngọc Linh Trương Thành Nhập Nguyễn Bằng Phi Nguyễn Ngọc Bảo Châu Nguyễn Bảo Quốc Identification of the rice blast fungus, Magnaporthe oryzae by the polymerase chain reaction (PCR) Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ pcr nấm đạo ôn bào tử pot2 transposon magnaporthe oryzae |
| title | Identification of the rice blast fungus, Magnaporthe oryzae by the polymerase chain reaction (PCR) |
| title_full | Identification of the rice blast fungus, Magnaporthe oryzae by the polymerase chain reaction (PCR) |
| title_fullStr | Identification of the rice blast fungus, Magnaporthe oryzae by the polymerase chain reaction (PCR) |
| title_full_unstemmed | Identification of the rice blast fungus, Magnaporthe oryzae by the polymerase chain reaction (PCR) |
| title_short | Identification of the rice blast fungus, Magnaporthe oryzae by the polymerase chain reaction (PCR) |
| title_sort | identification of the rice blast fungus magnaporthe oryzae by the polymerase chain reaction pcr |
| topic | pcr nấm đạo ôn bào tử pot2 transposon magnaporthe oryzae |
| url | https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/823 |
| work_keys_str_mv | AT đoanthihoa identificationofthericeblastfungusmagnaportheoryzaebythepolymerasechainreactionpcr AT vothingoclinh identificationofthericeblastfungusmagnaportheoryzaebythepolymerasechainreactionpcr AT truongthanhnhap identificationofthericeblastfungusmagnaportheoryzaebythepolymerasechainreactionpcr AT nguyenbangphi identificationofthericeblastfungusmagnaportheoryzaebythepolymerasechainreactionpcr AT nguyenngocbaochau identificationofthericeblastfungusmagnaportheoryzaebythepolymerasechainreactionpcr AT nguyenbaoquoc identificationofthericeblastfungusmagnaportheoryzaebythepolymerasechainreactionpcr |