Aeromonas sobria as a potential candidate for bioremediation of heavy metal from contaminated environments
Abstract The uncontrolled discharge of industrial wastes causes the accumulation of high heavy metal concentrations in soil and water, leading to many health issues. In the present study, a Gram-negative Aeromonas sobria was isolated from heavily contaminated soil in the Tanjaro area, southwest of S...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-25781-3 |
| _version_ | 1811302007153098752 |
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| author | Karzan Qurbani Karokh Khdir Avin Sidiq Haider Hamzah Safin Hussein Zhilia Hamad Rayan Abdulla Banw Abdulla Zahra Azizi |
| author_facet | Karzan Qurbani Karokh Khdir Avin Sidiq Haider Hamzah Safin Hussein Zhilia Hamad Rayan Abdulla Banw Abdulla Zahra Azizi |
| author_sort | Karzan Qurbani |
| collection | DOAJ |
| description | Abstract The uncontrolled discharge of industrial wastes causes the accumulation of high heavy metal concentrations in soil and water, leading to many health issues. In the present study, a Gram-negative Aeromonas sobria was isolated from heavily contaminated soil in the Tanjaro area, southwest of Sulaymaniyah city in the Kurdistan Region of Iraq; then, we assessed its ability to uptake heavy metals. A. sobria was molecularly identified based on the partial amplification of 16S rRNA using novel primers. The sequence was aligned with 33 strains to analyze phylogenetic relationships by maximum likelihood. Based on maximum tolerance concentration (MTC), A. sobria could withstand Zn, Cu, and Ni at concentrations of 5, 6, and 8 mM, respectively. ICP-OES data confirmed that A. sobria reduced 54.89% (0.549 mM) of the Cu, 62.33% (0.623 mM) of the Ni, and 36.41% (0.364 mM) of the Zn after 72 h in the culture medium. Transmission electron microscopy (TEM) showed that A. sobria accumulated both Cu and Ni, whereas biosorption was suggested for the Zn. These findings suggest that metal-resistant A. sobria could be a promising candidate for heavy metal bioremediation in polluted areas. However, more broadly, research is required to assess the feasibility of exploiting A. sobria in situ. |
| first_indexed | 2024-04-13T07:20:18Z |
| format | Article |
| id | doaj.art-65c2ced6e7294e00a0e4b57cf686b1f2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T07:20:18Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-65c2ced6e7294e00a0e4b57cf686b1f22022-12-22T02:56:38ZengNature PortfolioScientific Reports2045-23222022-12-011211810.1038/s41598-022-25781-3Aeromonas sobria as a potential candidate for bioremediation of heavy metal from contaminated environmentsKarzan Qurbani0Karokh Khdir1Avin Sidiq2Haider Hamzah3Safin Hussein4Zhilia Hamad5Rayan Abdulla6Banw Abdulla7Zahra Azizi8Department of Biology, College of Science, University of RaparinDepartment of Biology, College of Education, University of SulaimaniDepartment of Anesthesia, Faculty of Medical Sciences, Cihan UniversityDepartment of Biology, College of Science, University of SulaimaniDepartment of Biology, College of Science, University of RaparinDepartment of Biology, College of Science, University of RaparinDepartment of Biology, College of Science, University of RaparinDepartment of Biology, College of Science, University of RaparinDepartment of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical SciencesAbstract The uncontrolled discharge of industrial wastes causes the accumulation of high heavy metal concentrations in soil and water, leading to many health issues. In the present study, a Gram-negative Aeromonas sobria was isolated from heavily contaminated soil in the Tanjaro area, southwest of Sulaymaniyah city in the Kurdistan Region of Iraq; then, we assessed its ability to uptake heavy metals. A. sobria was molecularly identified based on the partial amplification of 16S rRNA using novel primers. The sequence was aligned with 33 strains to analyze phylogenetic relationships by maximum likelihood. Based on maximum tolerance concentration (MTC), A. sobria could withstand Zn, Cu, and Ni at concentrations of 5, 6, and 8 mM, respectively. ICP-OES data confirmed that A. sobria reduced 54.89% (0.549 mM) of the Cu, 62.33% (0.623 mM) of the Ni, and 36.41% (0.364 mM) of the Zn after 72 h in the culture medium. Transmission electron microscopy (TEM) showed that A. sobria accumulated both Cu and Ni, whereas biosorption was suggested for the Zn. These findings suggest that metal-resistant A. sobria could be a promising candidate for heavy metal bioremediation in polluted areas. However, more broadly, research is required to assess the feasibility of exploiting A. sobria in situ.https://doi.org/10.1038/s41598-022-25781-3 |
| spellingShingle | Karzan Qurbani Karokh Khdir Avin Sidiq Haider Hamzah Safin Hussein Zhilia Hamad Rayan Abdulla Banw Abdulla Zahra Azizi Aeromonas sobria as a potential candidate for bioremediation of heavy metal from contaminated environments Scientific Reports |
| title | Aeromonas sobria as a potential candidate for bioremediation of heavy metal from contaminated environments |
| title_full | Aeromonas sobria as a potential candidate for bioremediation of heavy metal from contaminated environments |
| title_fullStr | Aeromonas sobria as a potential candidate for bioremediation of heavy metal from contaminated environments |
| title_full_unstemmed | Aeromonas sobria as a potential candidate for bioremediation of heavy metal from contaminated environments |
| title_short | Aeromonas sobria as a potential candidate for bioremediation of heavy metal from contaminated environments |
| title_sort | aeromonas sobria as a potential candidate for bioremediation of heavy metal from contaminated environments |
| url | https://doi.org/10.1038/s41598-022-25781-3 |
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