Generation of hiPSC line TCIERi001-A from normal human epidermal keratinocytes

Human induced pluripotent stem cell (hiPSC) line TCIERi001-A was generated from normal human epidermal keratinocytes (NHEK) primary cell line with the nonintegrating system using Sendai reprogramming kit. Sendai particles were used to deliver the defined transcription factors that included three vec...

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Main Authors: Rupendra Shrestha, Yao-Tseng Wen, Rong-Kung Tsai
Format: Article
Language:English
Published: Elsevier 2019-12-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S187350611930220X
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author Rupendra Shrestha
Yao-Tseng Wen
Rong-Kung Tsai
author_facet Rupendra Shrestha
Yao-Tseng Wen
Rong-Kung Tsai
author_sort Rupendra Shrestha
collection DOAJ
description Human induced pluripotent stem cell (hiPSC) line TCIERi001-A was generated from normal human epidermal keratinocytes (NHEK) primary cell line with the nonintegrating system using Sendai reprogramming kit. Sendai particles were used to deliver the defined transcription factors that included three vector preparations, such as polycistronic KLF4–OCT3/4–SOX2, cMYC, and KLF4.
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spelling doaj.art-65c74ed7d1684eaf8a2de25340a63e7b2022-12-21T18:22:07ZengElsevierStem Cell Research1873-50612019-12-0141Generation of hiPSC line TCIERi001-A from normal human epidermal keratinocytesRupendra Shrestha0Yao-Tseng Wen1Rong-Kung Tsai2Institute of Medical Sciences, Tzu Chi University, Hualien 970, Taiwan; Institute of Eye Research, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, TaiwanInstitute of Eye Research, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, TaiwanInstitute of Medical Sciences, Tzu Chi University, Hualien 970, Taiwan; Institute of Eye Research, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan; Corresponding author at: Institute of Medical Sciences, Tzu Chi University, Hualien 970, Taiwan.Human induced pluripotent stem cell (hiPSC) line TCIERi001-A was generated from normal human epidermal keratinocytes (NHEK) primary cell line with the nonintegrating system using Sendai reprogramming kit. Sendai particles were used to deliver the defined transcription factors that included three vector preparations, such as polycistronic KLF4–OCT3/4–SOX2, cMYC, and KLF4.http://www.sciencedirect.com/science/article/pii/S187350611930220X
spellingShingle Rupendra Shrestha
Yao-Tseng Wen
Rong-Kung Tsai
Generation of hiPSC line TCIERi001-A from normal human epidermal keratinocytes
Stem Cell Research
title Generation of hiPSC line TCIERi001-A from normal human epidermal keratinocytes
title_full Generation of hiPSC line TCIERi001-A from normal human epidermal keratinocytes
title_fullStr Generation of hiPSC line TCIERi001-A from normal human epidermal keratinocytes
title_full_unstemmed Generation of hiPSC line TCIERi001-A from normal human epidermal keratinocytes
title_short Generation of hiPSC line TCIERi001-A from normal human epidermal keratinocytes
title_sort generation of hipsc line tcieri001 a from normal human epidermal keratinocytes
url http://www.sciencedirect.com/science/article/pii/S187350611930220X
work_keys_str_mv AT rupendrashrestha generationofhipsclinetcieri001afromnormalhumanepidermalkeratinocytes
AT yaotsengwen generationofhipsclinetcieri001afromnormalhumanepidermalkeratinocytes
AT rongkungtsai generationofhipsclinetcieri001afromnormalhumanepidermalkeratinocytes