Meta-omics revealed that nitrogen fertilization alters the endophytic fungi divergence in maize field ecosystem
Endophytic mycobiomes are an integral part of crops in agricultural systems. Fertilization is a conventional practice to improve agricultural crops and may alter the mycobiomes of soil and plant systems. In agroecosystems, Nature Based Solutions (NBS) are key strategies for agricultural suitability....
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
|
| Series: | Ecological Indicators |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1470160X24003091 |
| _version_ | 1797213457312907264 |
|---|---|
| author | Lara Sindhu Yingying Song Lili Li Zhaoke Dong Quanfeng Yang Sorath Sindhu Mangi Wenxiu Guo Lujia Yang Hongying Cui Suhong Lv Yi Yu Xingyuan Men |
| author_facet | Lara Sindhu Yingying Song Lili Li Zhaoke Dong Quanfeng Yang Sorath Sindhu Mangi Wenxiu Guo Lujia Yang Hongying Cui Suhong Lv Yi Yu Xingyuan Men |
| author_sort | Lara Sindhu |
| collection | DOAJ |
| description | Endophytic mycobiomes are an integral part of crops in agricultural systems. Fertilization is a conventional practice to improve agricultural crops and may alter the mycobiomes of soil and plant systems. In agroecosystems, Nature Based Solutions (NBS) are key strategies for agricultural suitability. Investigating mycobiomes under N flux can provide fundamental data for constructive NBS strategies. In this study, the effects of nitrogen (N) fertilizer treatments on fungal compositions of the soil-maize systems were systematically investigated in field experiments at six N rates (F0, F72, F126, F180, F234, F280 kg N/ha) in northern China. Meta-omics amplicon sequencing of the internal transcribed spacer (ITS) region was employed to study community dynamics, network correlation, metabolic functional assemblies, abundance patterns and core fungi. This study found that fungal communities were significantly higher at 72 kg N/ha compared with control and other treatments. Network topology increased in maize fungi (R2 = 0.69) but decreased in the soil (R2 = 0.51). Abundances of carbon–nitrogen metabolizing functional genes (Ure, AMT, gdhA and GDH) were significantly correlated with maize fungal communities and were assembled by stochastic processes. Additionally, the relative abundance of Ascomycota (67.26 %) was dominant in the soil, whereas Basidiomycota (61. 73 %) prevailed in maize samples at 72 kg N/ha. Moreover, Sporidiobolus, Alternaria, Fusarium, and Penicillium served as conserved core genera and were positively correlated with NH4+-N mg/kg, NO2–-N mg/kg and NO3–-N mg/kg, and pH components. Furthermore, selective genera were tested and confirmed to increase the growth rate and nitrogen utilization in maize. The study highlights the promising role of associated endophytes as Nature-Based Solution for optimizing fertilization to improve crop production in maize-agroecosystem. |
| first_indexed | 2024-04-24T10:58:35Z |
| format | Article |
| id | doaj.art-65c7ac342f7043d5bcb2df912b1af6e6 |
| institution | Directory Open Access Journal |
| issn | 1470-160X |
| language | English |
| last_indexed | 2024-04-24T10:58:35Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecological Indicators |
| spelling | doaj.art-65c7ac342f7043d5bcb2df912b1af6e62024-04-12T04:44:46ZengElsevierEcological Indicators1470-160X2024-03-01160111852Meta-omics revealed that nitrogen fertilization alters the endophytic fungi divergence in maize field ecosystemLara Sindhu0Yingying Song1Lili Li2Zhaoke Dong3Quanfeng Yang4Sorath Sindhu Mangi5Wenxiu Guo6Lujia Yang7Hongying Cui8Suhong Lv9Yi Yu10Xingyuan Men11Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaKey Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, ChinaKey Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, ChinaInstitute of Pathology, Liaquat University of Medical & Health Sciences, PakistanInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, China; Corresponding author.Endophytic mycobiomes are an integral part of crops in agricultural systems. Fertilization is a conventional practice to improve agricultural crops and may alter the mycobiomes of soil and plant systems. In agroecosystems, Nature Based Solutions (NBS) are key strategies for agricultural suitability. Investigating mycobiomes under N flux can provide fundamental data for constructive NBS strategies. In this study, the effects of nitrogen (N) fertilizer treatments on fungal compositions of the soil-maize systems were systematically investigated in field experiments at six N rates (F0, F72, F126, F180, F234, F280 kg N/ha) in northern China. Meta-omics amplicon sequencing of the internal transcribed spacer (ITS) region was employed to study community dynamics, network correlation, metabolic functional assemblies, abundance patterns and core fungi. This study found that fungal communities were significantly higher at 72 kg N/ha compared with control and other treatments. Network topology increased in maize fungi (R2 = 0.69) but decreased in the soil (R2 = 0.51). Abundances of carbon–nitrogen metabolizing functional genes (Ure, AMT, gdhA and GDH) were significantly correlated with maize fungal communities and were assembled by stochastic processes. Additionally, the relative abundance of Ascomycota (67.26 %) was dominant in the soil, whereas Basidiomycota (61. 73 %) prevailed in maize samples at 72 kg N/ha. Moreover, Sporidiobolus, Alternaria, Fusarium, and Penicillium served as conserved core genera and were positively correlated with NH4+-N mg/kg, NO2–-N mg/kg and NO3–-N mg/kg, and pH components. Furthermore, selective genera were tested and confirmed to increase the growth rate and nitrogen utilization in maize. The study highlights the promising role of associated endophytes as Nature-Based Solution for optimizing fertilization to improve crop production in maize-agroecosystem.http://www.sciencedirect.com/science/article/pii/S1470160X24003091AgroecosystemNutrient cyclingFungi-community-networkEcological driversFunctional assembly |
| spellingShingle | Lara Sindhu Yingying Song Lili Li Zhaoke Dong Quanfeng Yang Sorath Sindhu Mangi Wenxiu Guo Lujia Yang Hongying Cui Suhong Lv Yi Yu Xingyuan Men Meta-omics revealed that nitrogen fertilization alters the endophytic fungi divergence in maize field ecosystem Ecological Indicators Agroecosystem Nutrient cycling Fungi-community-network Ecological drivers Functional assembly |
| title | Meta-omics revealed that nitrogen fertilization alters the endophytic fungi divergence in maize field ecosystem |
| title_full | Meta-omics revealed that nitrogen fertilization alters the endophytic fungi divergence in maize field ecosystem |
| title_fullStr | Meta-omics revealed that nitrogen fertilization alters the endophytic fungi divergence in maize field ecosystem |
| title_full_unstemmed | Meta-omics revealed that nitrogen fertilization alters the endophytic fungi divergence in maize field ecosystem |
| title_short | Meta-omics revealed that nitrogen fertilization alters the endophytic fungi divergence in maize field ecosystem |
| title_sort | meta omics revealed that nitrogen fertilization alters the endophytic fungi divergence in maize field ecosystem |
| topic | Agroecosystem Nutrient cycling Fungi-community-network Ecological drivers Functional assembly |
| url | http://www.sciencedirect.com/science/article/pii/S1470160X24003091 |
| work_keys_str_mv | AT larasindhu metaomicsrevealedthatnitrogenfertilizationalterstheendophyticfungidivergenceinmaizefieldecosystem AT yingyingsong metaomicsrevealedthatnitrogenfertilizationalterstheendophyticfungidivergenceinmaizefieldecosystem AT lilili metaomicsrevealedthatnitrogenfertilizationalterstheendophyticfungidivergenceinmaizefieldecosystem AT zhaokedong metaomicsrevealedthatnitrogenfertilizationalterstheendophyticfungidivergenceinmaizefieldecosystem AT quanfengyang metaomicsrevealedthatnitrogenfertilizationalterstheendophyticfungidivergenceinmaizefieldecosystem AT sorathsindhumangi metaomicsrevealedthatnitrogenfertilizationalterstheendophyticfungidivergenceinmaizefieldecosystem AT wenxiuguo metaomicsrevealedthatnitrogenfertilizationalterstheendophyticfungidivergenceinmaizefieldecosystem AT lujiayang metaomicsrevealedthatnitrogenfertilizationalterstheendophyticfungidivergenceinmaizefieldecosystem AT hongyingcui metaomicsrevealedthatnitrogenfertilizationalterstheendophyticfungidivergenceinmaizefieldecosystem AT suhonglv metaomicsrevealedthatnitrogenfertilizationalterstheendophyticfungidivergenceinmaizefieldecosystem AT yiyu metaomicsrevealedthatnitrogenfertilizationalterstheendophyticfungidivergenceinmaizefieldecosystem AT xingyuanmen metaomicsrevealedthatnitrogenfertilizationalterstheendophyticfungidivergenceinmaizefieldecosystem |